BMP3 Affects Cortical and Trabecular Long Bone Development in Mice

Scientific paper - Original scientific paper

• Author: Ivan Banovac
• Author: Lovorka Grgurević
• Author: Viktorija Rumenović
• Author: Slobodan Vukičević
• Author: Igor Erjavec
• Author's institution: University of Zagreb; School of Medicine; (Department of Anatomy and Clinical Anatomy)
• Scientific / art field, discipline and subdiscipline: BIOMEDICINE AND HEALTHCARE; Basic Medical Sciences; Anatomy

Abstract (english)

Bone morphogenetic proteins (BMPs) have a major role in tissue development. BMP3 is synthesized in osteocytes and mature osteoblasts and has an antagonistic effect on other BMPs in bone tissue. The main aim of this study was to fully characterize cortical bone and trabecular bone of long bones in both male and female Bmp3−/− mice. To investigate the effect of Bmp3 from birth to maturity, we compared Bmp3−/− mice with wild-type littermates at the following stages of postnatal development: 1 day (P0), 2 weeks (P14), 8 weeks and 16 weeks of age. Bmp3 deletion was confirmed using X-gal staining in P0 animals. Cartilage and bone tissue were examined in P14 animals using Alcian Blue/Alizarin Red staining. Detailed long bone analysis was performed in 8-week-old and 16-week-old animals using micro-CT. The Bmp3 reporter signal was localized in bone tissue, hair follicles, and lungs. Bone mineralization at 2 weeks of age was increased in long bones of Bmp3−/− mice. Bmp3 deletion was shown to affect the skeleton until adulthood, where increased cortical and trabecular bone parameters were found in young and adult mice of both sexes, while delayed mineralization of the epiphyseal growth plate was found in adult Bmp3−/− mice.

• Keywords (english): Age Factors; Animals; Biomarkers; Bone Morphogenetic Protein 3 / genetics; Bone Morphogenetic Protein 3 / metabolism; Bone and Bones / metabolism; Calcification, Physiologic; Cortical Bone / metabolism; Female; Gene Expression; Growth Plate / growth & development; Growth Plate / metabolism; Immunohistochemistry; Male; Mice; Mice, Knockout; Osteogenesis / genetics; Sex Factors; X-Ray Microtomography
• Language: english
• Publication type: Scientific paper - Original scientific paper
• Publication status: Published
• Peer review: Peer review
• Publication version: Published version
• Journal title: International Journal of Molecular Sciences
• Numbering: vol. 23, no. 2, 785
• e-ISSN: 1422-0067
• DOI: https://doi.org/10.3390/ijms23020785
• URN:NBN: urn:nbn:hr:105:969339
• Publication: 2022-01-12
• Project: Number: KK.01.1.1.01.0008; Title (croatian): Znanstveni centar izvrsnosti za reproduktivnu i regenerativnu medicinu – istraživanja novih platformi i potencijala; Title (english): Scientific Center of Excellence for Reproductive and Regenerative Medicine; Acronym: CERRM; Leader: Davor JežekScientific Center of Excellence for Reproductive and Regenerative Medicine; Jurisdiction: eu
• Document URL: https://www.mdpi.com/1422-0067/23/2/785
• Type of resource: Text
• Access conditions: Open access
• Created on: 2022-07-01 06:34:21