prikaz prve stranice dokumenta Extended Prophylactic Effect of N-tert-Butyl-α-phenylnitron against Oxidative/Nitrosative Damage Caused by the DNA-Hypomethylating Drug 5-Azacytidine in the Rat Placenta
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Scientific paper - Original scientific paper
Extended Prophylactic Effect of N-tert-Butyl-α-phenylnitron against Oxidative/Nitrosative Damage Caused by the DNA-Hypomethylating Drug 5-Azacytidine in the Rat Placenta
International Journal of Molecular Sciences, 23 (2022), 2; 603. https://doi.org/10.3390/ijms23020603
Sobočan, Nikola; Himelreich-Perić, Marta; Katušić-Bojanac, Ana; Krasić, Jure; Sinčić, Nino; Majić, Željka; Jurić-Lekić, Gordana; Šerman, Ljiljana; Marić, Andreja; Ježek, Davor; Bulić-Jakuš, Floriana

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Sobočan, N., Himelreich Perić, M., Katušić Bojanac, A., Krasić, J., Sinčić, N., Majić, Ž. ... Bulić Jakuš, F. (2022). Extended Prophylactic Effect of N-tert-Butyl-α-phenylnitron against Oxidative/Nitrosative Damage Caused by the DNA-Hypomethylating Drug 5-Azacytidine in the Rat Placenta. International Journal of Molecular Sciences, 23. (2). doi: 10.3390/ijms23020603

Sobočan, Nikola, et al. "Extended Prophylactic Effect of N-tert-Butyl-α-phenylnitron against Oxidative/Nitrosative Damage Caused by the DNA-Hypomethylating Drug 5-Azacytidine in the Rat Placenta." International Journal of Molecular Sciences, vol. 23, no. 2, 2022. https://doi.org/10.3390/ijms23020603

Sobočan, Nikola, Marta Himelreich Perić, Ana Katušić Bojanac, Jure Krasić, Nino Sinčić, Željka Majić, Gordana Jurić Lekić, et al. "Extended Prophylactic Effect of N-tert-Butyl-α-phenylnitron against Oxidative/Nitrosative Damage Caused by the DNA-Hypomethylating Drug 5-Azacytidine in the Rat Placenta." International Journal of Molecular Sciences 23, no. 2 (2022). https://doi.org/10.3390/ijms23020603

Sobočan, N., et al. (2022) 'Extended Prophylactic Effect of N-tert-Butyl-α-phenylnitron against Oxidative/Nitrosative Damage Caused by the DNA-Hypomethylating Drug 5-Azacytidine in the Rat Placenta', International Journal of Molecular Sciences, 23(2). doi: 10.3390/ijms23020603

Sobočan N, Himelreich Perić M, Katušić Bojanac A, Krasić J, Sinčić N, Majić Ž, and sur.. Extended Prophylactic Effect of N-tert-Butyl-α-phenylnitron against Oxidative/Nitrosative Damage Caused by the DNA-Hypomethylating Drug 5-Azacytidine in the Rat Placenta. International Journal of Molecular Sciences [Internet]. 2022 January 06 [cited 2024 December 20];23(2). doi: 10.3390/ijms23020603

N. Sobočan, et al., "Extended Prophylactic Effect of N-tert-Butyl-α-phenylnitron against Oxidative/Nitrosative Damage Caused by the DNA-Hypomethylating Drug 5-Azacytidine in the Rat Placenta", International Journal of Molecular Sciences, vol. 23, no. 2, January 2022. [Online]. Available at: https://urn.nsk.hr/urn:nbn:hr:105:168102. [Accessed: 20 December 2024]

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Metadata
Title (english)Extended Prophylactic Effect of N-tert-Butyl-α-phenylnitron against Oxidative/Nitrosative Damage Caused by the DNA-Hypomethylating Drug 5-Azacytidine in the Rat Placenta
AuthorNikola Sobočan
AuthorMarta Himelreich-Perić
AuthorAna Katušić-Bojanac
AuthorJure Krasić
AuthorNino Sinčić
AuthorŽeljka Majić
AuthorGordana Jurić-Lekić
AuthorLjiljana Šerman
AuthorAndreja Marić
AuthorDavor Ježek
AuthorFloriana Bulić-Jakuš
Author's institutionUniversity of Zagreb
School of Medicine
(Department of Histology and Embryology)
Author's institutionUniversity of Zagreb
School of Medicine
(Department of Medical Biology)
Scientific / art field,
discipline and subdiscipline		BIOMEDICINE AND HEALTHCARE
Basic Medical Sciences
Cytology, Histology and Embryology
Abstract (english)
Antioxidant N-tert-Butyl-α-phenylnitron (PBN) partly protected embryos from the negative effects of a DNA demethylating drug 5-azacytidine during pregnancy. Our aim was to investigate PBN’s impact on the placenta. Fischer rat dams were treated on gestation days (GD) 12 and 13 by PBN (40 mg/kg), followed by 5azaC (5 mg/kg) after one hour. Global methylation was assessed by pyrosequencing. Numerical density was calculated from immunohistochemical expression in single cells for proliferating (PCNA), oxidative (oxoguanosine) and nitrosative (nitrotyrosine) activity. Results were compared with the PBN-treated and control rats. PBN-pretreatment significantly increased placental weight at GD15 and GD20, diminished by 5azaC, and diminished apoptosis in GD 20 placentas caused by 5azaC. Oxoguanosine expression in placentas of 5azaC-treated dams was especially high in the placental labyrinth on GD 15, while PBN-pretreatment lowered its expression on GD 15 and GD 20 in both the labyrinth and basal layer. 5azaC enhanced nitrotyrosine level in the labyrinth of both gestational stages, while PBN-pretreatment lowered it. We conclude that PBN exerted its prophylactic activity against DNA hypomethylating agent 5azaC in the placenta through free radical scavenging, especially in the labyrinthine part of the placenta until the last day of pregnancy.
Keywords (english)
Languageenglish
Publication typeScientific paper - Original scientific paper
Publication statusPublished
Peer reviewPeer review
Publication versionPublished version
Journal titleInternational Journal of Molecular Sciences
Numberingvol. 23, no. 2, 603
e-ISSN1422-0067
DOIhttps://doi.org/10.3390/ijms23020603
URN:NBNurn:nbn:hr:105:168102
Publication2022-01-06
ProjectNumber: KK.01.1.1.01.0008
Title (croatian): Reproduktivna i regenerativna medicina - istraživanje novih platformi i potencijala
Leader: Ana Katušić Bojanac
Jurisdiction: eu
ProjectNumber: 108-1080399-0335
Title (croatian): Eksperimentalni embrionalni tumori i razvoj zametaka sisavaca in vitro i in vivo
Title (english): Experimental embryonic tumors and mammalian development in vitro and in vivo
Leader: Florijana Jakuš
Jurisdiction: Croatia
Funding stream: ZP
Document URLhttps://www.mdpi.com/1422-0067/23/2/603
Type of resourceText
Access conditionsOpen access
Terms of useLicence Attribution 4.0 International (CC BY 4.0) icon
Created on2022-11-15 09:31:55