Presječno istraživanje koncentracije grelina, leptina i proupalnih citokina u serumu bolesnika s upalnim bolestima crijeva

doctoral thesis

• Title (english): Cross sectional study of serum ghrelin, leptin and proinflammatory cytokines levels in inflammatory bowel disease
• Author: Ana Barišić
• Mentor: Željko Krznarić (mentor)
• Committee member: Neven Ljubičić (predsjednik povjerenstva)
• Committee member: Marko Banić (član povjerenstva)
• Committee member: Žarko Babić (član povjerenstva)
• Granter: University of Zagreb; School of Medicine; Zagreb
• Defense date and country: 2022-11-16, Croatia
• Scientific / art field, discipline and subdiscipline: BIOMEDICINE AND HEALTHCARE; Clinical Medical Sciences; Internal Medicine
• Universal decimal classification (UDC): 616 - Pathology. Clinical medicine

Abstract

Upalne bolesti crijeva (UBC) kronične su bolesti čiji se porast incidencija prati u
posljednjih nekoliko godina. Stoga ne čudi da je velik broj istraživanja u UBC-u
usmjeren ka otkrivanju biomarkera koji bi bio koristan u praćenju aktivnosti bolesti.
Kako u upalnim bolestima crijeva dolazi do čitavog niza metaboličkih promjena,
moguće su fluktuacije proizvodnje grelina i leptina, te bi upravo njihove serumske
razine mogle predstavljati potencijalne biomarkere u UBC-u. Osim toga, citokini su
često istraživani biomarkeri u UBC-u, s obzirom da su upravo oni ključne
komponente u upalnim putevima važnima u patogenezi UBC-a. Cilj našeg
istraživanja bio je odrediti serumske koncentracije grelina, leptina, TNF-α i IL-6 u
UBC-u te istražiti povezanost njihovih razina s aktivnosti bolesti i nutritivnim
statusom. Rezultati istraživanja su pokazali da bolesnici s UBC-om imaju niže
vrijednosti leptina, a više vrijednosti TNF-α u odnosu na kontrolnu skupinu. Dokazana
je pozitivna korelacija IL-6 s laboratorijskim pokazateljima upalne aktivnosti, te s
kliničkom aktivnosti UBC-a. Osim toga, dokazali smo pozitivnu korelaciju leptina s
masom masnog tkiva, stopom visceralnog masnog tkiva te indeksom tjelesne mase
te njegovu negativnu korelaciju sa skeletnom mišićnom masom u UBC-u. Razine
grelina u većini naših uzoraka bile su nedetektabilne, što smo objasnili greškom u
metodologiji. U našem istraživanju IL-6 se pokazao kao potencijalni biomarker u
praćenju aktivnosti bolesti, a leptin kao dobar pokazatelj nutritivnog statusa.
Međutim, potencijalnu ulogu ispitivanih biomarkera u upalnim bolestima crijeva
potrebno je reevaluirati na većem i homogenijem uzorku ispitanika.

Abstract (english)

Inflammatory bowel disease (IBD) is a chronic disease with an increasing incidence
in the past few years. Much research in the IBD field has focused on finding reliable
serum biomarkers for assessing disease activity. IBD is associated with several
metabolic changes that may lead to fluctuation of ghrelin and leptin production.
Therefore, serum ghrelin and leptin levels may represent potential biomarkers in IBD.
Furthermore, cytokines are commonly researched biomarkers in IBD, due to the fact
they have been identified as the key players in intestinal inflammation. Our research
has aimed to investigate serum ghrelin, leptin, TNF-α, and IL-6 concentrations in IBD
and the association between their levels and activity of the disease and nutritional
status. Our results suggest that patients with IBD have lower levels of leptin, and
higher levels of TNF-α in comparison with the control group. It has been shown that
IL-6 positively correlates with laboratory and clinical markers of disease activity. In
addition, leptin levels have been found to exhibit a positive correlate with levels of fat
mass, visceral fat mass, and body mass index. A negative correlation between leptin
levels and skeletal muscle mass in IBD patients has been proven. Systematic
measurement errors are a probable cause of undetectable levels of ghrelin in our
research. Finally, IL-6 has been shown as a potential biomarker of disease activity.
However, additional studies with larger homogenous cohorts may be needed to
validate the value of tested biomarkers as non-invasive biomarkers of IBD activity.

• Keywords: upalne bolesti crijeva; grelin; leptin; faktor tumorske nekroze-α (TNF-α); interleukin-6 (IL-6)
• Keywords (english): inflammatory bowel disease; Ghrelin; Leptin; Tumor necrosis factor α (TNF-α); Interleukin-6 (IL-6)
• Language: croatian
• URN:NBN: urn:nbn:hr:105:203537
• Study programme: Title: Biomedicine and Health Sciences; Study programme type: university; Study level: postgraduate; Academic / professional title: doktor/doktorica znanosti, područje biomedicine i zdravstvo (doktor/doktorica znanosti, područje biomedicine i zdravstvo)
• Type of resource: Text
• Extent: 99 str.
• File origin: Born digital
• Access conditions: Open access
• Created on: 2023-01-12 09:57:44