Methylation pattern of Caveolin-1 in prostate cancer as potential cfDNA biomarker

Scientific paper - Original scientific paper

• Author: Lucija Škara
• Author: Tonći Vodopić
• Author: Ivan Pezelj
• Author: Irena Abramović
• Author: Borna Vrhovec
• Author: Alen Vrtarić
• Author: Nino Sinčić
• Author: Davor Tomas
• Author: Stela Bulimbašić
• Author: Tomislav Kuliš
• Author: Monika Ulamec
• Author's institution: University of Zagreb; School of Medicine; (Department of Medical Biology)
• Author's institution: University of Zagreb; School of Medicine; (Department of Pathology)
• Author's institution: University of Zagreb; School of Medicine; (Department of Urology)
• Scientific / art field, discipline and subdiscipline: BIOMEDICINE AND HEALTHCARE; Clinical Medical Sciences; Pathology
• Scientific / art field, discipline and subdiscipline: BIOMEDICINE AND HEALTHCARE; Clinical Medical Sciences; Urology

Abstract (english)

High prevalence and mortality of prostate cancer (PCa) are well known global health issues. Novel biomarkers for better identifying patients with PCa are the subject of extensive research. Prostate specific antigen (PSA) shows low specificity in screening and diagnostics, leading to unnecessary biopsies and health costs. Eighty patients with PCa and benign prostate hyperplasia (BPH) were included in the study. We analyzed CAV1 gene expression and methylation in tissue. CAV1 cfDNA methylation from blood and seminal plasma was accessed as a potential PCa biomarker. Although methylation in blood plasma did not differ between PCa and BPH patients, methylation in seminal plasma showed better PCa biomarker performances than tPSA (AUC 0.63 vs. AUC 0.52). Discrimination of BPH and Gleason grade group 1 PCa patients from patients with higher Gleason grade groups revealed very good performance as well (AUC 0.72). CAV1 methylation is useful biomarker with potential for further seminal plasma cfDNA research, but its diagnostic accuracy should be improved, as well as general knowledge about cfDNA in seminal plasma.

• Keywords (english): Biomarkers / metabolism; Caveolin 1 / genetics; Cell-Free Nucleic Acids / metabolism; Humans; Male; Methylation; Prostatic Hyperplasia; Prostatic Neoplasms / diagnosis
• Language: english
• Publication type: Scientific paper - Original scientific paper
• Publication status: Published
• Peer review: Peer review
• Publication version: Published version
• Journal title: Bosnian Journal of Basic Medical Sciences
• Numbering: vol. 23, no. 1, pp. 176-186
• p-ISSN: 1512-8601
• e-ISSN: 1840-4812
• DOI: https://doi.org/10.17305/bjbms.2022.7497
• URN:NBN: urn:nbn:hr:105:191297
• Publication: 2022-02-01
• Document URL: https://www.bjbms.org/ojs/index.php/bjbms/article/view/7497
• Type of resource: Text
• Access conditions: Open access
• Created on: 2023-03-01 16:36:11