prikaz prve stranice dokumenta Valproate Targets Mammalian Gastrulation Impairing Neural Tissue Differentiation and Development of the Placental Source In Vitro
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Scientific paper - Original scientific paper
Valproate Targets Mammalian Gastrulation Impairing Neural Tissue Differentiation and Development of the Placental Source In Vitro
International Journal of Molecular Sciences, 23 (2022), 16; 8861. https://doi.org/10.3390/ijms23168861
Katušić-Bojanac, Ana; Plazibat, Milvija; Himelreich-Perić, Marta; Eck-Raković, Katarina; Krasić, Jure; Sinčić, Nino; Jurić-Lekić, Gordana; Ježek, Davor; Bulić-Jakuš, Floriana

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Katušić Bojanac, A., Plazibat, M., Himelreich Perić, M., Eck Raković, K., Krasić, J., Sinčić, N. ... Bulić Jakuš, F. (2022). Valproate Targets Mammalian Gastrulation Impairing Neural Tissue Differentiation and Development of the Placental Source In Vitro. International Journal of Molecular Sciences, 23. (16). doi: 10.3390/ijms23168861

Katušić Bojanac, Ana, et al. "Valproate Targets Mammalian Gastrulation Impairing Neural Tissue Differentiation and Development of the Placental Source In Vitro." International Journal of Molecular Sciences, vol. 23, no. 16, 2022. https://doi.org/10.3390/ijms23168861

Katušić Bojanac, Ana, Milvija Plazibat, Marta Himelreich Perić, Katarina Eck Raković, Jure Krasić, Nino Sinčić, Gordana Jurić Lekić, Davor Ježek and Floriana Bulić Jakuš. "Valproate Targets Mammalian Gastrulation Impairing Neural Tissue Differentiation and Development of the Placental Source In Vitro." International Journal of Molecular Sciences 23, no. 16 (2022). https://doi.org/10.3390/ijms23168861

Katušić Bojanac, A., et al. (2022) 'Valproate Targets Mammalian Gastrulation Impairing Neural Tissue Differentiation and Development of the Placental Source In Vitro', International Journal of Molecular Sciences, 23(16). doi: 10.3390/ijms23168861

Katušić Bojanac A, Plazibat M, Himelreich Perić M, Eck Raković K, Krasić J, Sinčić N, and sur.. Valproate Targets Mammalian Gastrulation Impairing Neural Tissue Differentiation and Development of the Placental Source In Vitro. International Journal of Molecular Sciences [Internet]. 2022 August 09 [cited 2024 September 12];23(16). doi: 10.3390/ijms23168861

A. Katušić Bojanac, et al., "Valproate Targets Mammalian Gastrulation Impairing Neural Tissue Differentiation and Development of the Placental Source In Vitro", International Journal of Molecular Sciences, vol. 23, no. 16, August 2022. [Online]. Available at: https://urn.nsk.hr/urn:nbn:hr:105:001479. [Accessed: 12 September 2024]

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Title (english)Valproate Targets Mammalian Gastrulation Impairing Neural Tissue Differentiation and Development of the Placental Source In Vitro
AuthorAna Katušić-Bojanac
AuthorMilvija Plazibat
AuthorMarta Himelreich-Perić
AuthorKatarina Eck-Raković
AuthorJure Krasić
AuthorNino Sinčić
AuthorGordana Jurić-Lekić
AuthorDavor Ježek
AuthorFloriana Bulić-Jakuš
Author's institutionUniversity of Zagreb
School of Medicine
(Department of Histology and Embryology)
Author's institutionUniversity of Zagreb
School of Medicine
(Department of Medical Biology)
Scientific / art field,
discipline and subdiscipline		BIOMEDICINE AND HEALTHCARE
Basic Medical Sciences
Cytology, Histology and Embryology
Abstract (english)
The teratogenic activity of valproate (VPA), an antiepileptic and an inhibitor of histone deacetylase (HDACi), is dose-dependent in humans. Previous results showed that VPA impairs in vitro development and neural differentiation of the gastrulating embryo proper. We aimed to investigate the impact of a lower VPA dose in vitro and whether this effect is retained in transplants in vivo. Rat embryos proper (E9.5) and ectoplacental cones were separately cultivated at the air-liquid interface with or without 1 mM VPA. Embryos were additionally cultivated with HDACi Trichostatin A (TSA), while some cultures were syngeneically transplanted under the kidney capsule for 14 days. Embryos were subjected to routine histology, immunohistochemistry, Western blotting and pyrosequencing. The overall growth of VPA-treated embryos in vitro was significantly impaired. However, no differences in the apoptosis or proliferation index were found. Incidence of the neural tissue was lower in VPA-treated embryos than in controls. TSA also impaired growth and neural differentiation in vitro. VPA-treated embryos and their subsequent transplants expressed a marker of undifferentiated neural cells compared to controls where neural differentiation markers were expressed. VPA increased the acetylation of histones. Our results point to gastrulation as a sensitive period for neurodevelopmental impairment caused by VPA.
Keywords (english)
Languageenglish
Publication typeScientific paper - Original scientific paper
Publication statusPublished
Peer reviewPeer review
Publication versionPublished version
Journal titleInternational Journal of Molecular Sciences
Numberingvol. 23, no. 16, 8861
e-ISSN1422-0067
DOIhttps://doi.org/10.3390/ijms23168861
URN:NBNurn:nbn:hr:105:001479
Publication2022-08-09
Document URLhttps://www.mdpi.com/1422-0067/23/16/8861
Type of resourceText
Access conditionsOpen access
Terms of useLicence Attribution 4.0 International (CC BY 4.0) icon
Created on2023-03-23 12:38:54