Promjene razine izotipova specifičih imunoglobulina u atopičara i zdravih ispitanika prije i tijekom prirodne izloženosti peludu biljke Ambrosia elatior L.

doctoral thesis

• Author: Vedran Ostojić
• Mentor: Asja Stipić Marković (mentor)
• Mentor: Drago Batinić (komentor)
• Committee member: nije dostupno (član povjerenstva)
• Granter: University of Zagreb; School of Medicine; Zagreb
• Defense date and country: 2019-12-20, Croatia
• Scientific / art field, discipline and subdiscipline: BIOMEDICINE AND HEALTHCARE; Clinical Medical Sciences
• Universal decimal classification (UDC): 61 - Medical sciences

Abstract

Fiziološki način reagiranja na alergene nije do kraja poznat u zdravih ispitanika niti je
do kraja poznat imunopatološki mehanizam u bolesnika s alergijom. Unatoč
prihvaćenoj teoriji o Th1-tipu imunološkog odgovora u zdravih ispitanika koji bi trebao
rezultirati stvaranjem "blokirajućih protutijela" Th1 smjera i time dovesti do izostanka
kliničke slike alergijske bolesti, u ovom istraživanju nije uočen takav porast tijekom
prirodne ekspozicije peludi biljke Ambrosia elatior. Zabilježen je pad serumske razine
imunoglobulina sIgG2 u zdravih ispitanika, što bi, teoretski, moglo imati ulogu u
neutralizaciji cirkulirajućeg alergena.
Osobitosti humoralne imunosti u osoba koje boluju od alergijskih bolesti tijekom
prirodne ekspozicije peludi biljke Ambrosia elatior nije samo porast sIgE, već i porast
drugih razreda i podrazreda imunoglobulina koji se tradicionalno svrstavaju u Th1
smjer (A1, G3, G4).
Rezultati ovoga istraživanja pokazuju kako prirodna ekspozicija alergenu iz okoliša
ima u alergične osobe sličan učinak na porast sIgG4 kao i primjena
hiposenzibilizacije. Stoga porast IgG4 vjerojatno nije sam po sebi mehanizam koji
dovodi do kliničkog poboljšanja u bolesnika na hiposenzibilizaciji. To je još jedan
argument koji, zajedno s visokom razinom serumskog alergen-specifičnoga sIgM
govori protiv uvriježene "teorije blokirajućih protutijela".
Dobiveni rezultati bi mogli usmjeriti daljnja istraživanja u bolesnika s alergijom, prije
svega istraživanja cjelovitosti sluzničkih barijera, dinamike imunoglobulinskih razreda
i podrazreda u drugim tjelesnim uzorcima (ispirku nosa i bioptatu sluznice),
određivanju razine alergena u krvi i određivanju sIgE ovom kvantitativnom metodom,
različitosti razina sIgM i drugih sIg protutijela u bolesnika s anafilaksijom, potencijalnu
terapijsku primjenu sIgG2, kao i istraživanje imunoloških stanica i njihovih lučevina.
Na taj bi se način dobila cjelovitija slika imunoloških zbivanja na alergene u zdravih
osoba i bolesnika s alergijom.

Abstract (english)

The physiologic way of responding to allergens in healthy subjects is not fully
understood, nor the immunopathological mechanism in patients with allergies.
Despite the accepted theory of Th1-type immune response in healthy subjects that
should result in the formation of "blocking antibodies" and thus the absence of a
clinical symptoms of allergic disease, no such increase was observed during the
natural exposure of the Ambrosia elatior plant pollen. For one immunoglobulin class
(sIgG2) slight fall in serum levels was shown, which could, theoretically, play a role in
neutralizing the circulating allergen.
The characteristics of humoral immunity in people with allergic diseases during the
natural exposure of the pollen of the Ambrosia elatior plant is not only an increase in
sIgE levels but also increase in some other classes and subclasses, traditionaly
classified as Th1 immunoglobulin (A1, G3, G4).
The results of this study show that the natural exposure of allergic persons to the
environmental allergen has the similar effect on increase of sIgG4 in allergic persons
comparable to those in hyposensitisation. Therefore, the rise of IgG4 is probably not
by itself a mechanism that leads to clinical improvement in hyposenzitized patients.
That argument, together with the high level of allergen-specific serum sIgM levels
and also other imunoglobuline classes, dispute against the "theory of blocking
antibodies".
The results obtained could be used for further research in patients with allergy,
primarily the investigation of total barrier function, dynamics of immunoglobulin
classes and subclasses in other body specimens (nose and mucous membrane
biopsies), determination of blood allergen levels and determination of sIgE using this
quantitative method, possible differences in sIgM and other sIg levels in patients with
anaphyllaxis, potential therapeutic application of sIgG2 antibodies as well as
research in immune cells and their mediators. That way, a more complete insight
about immune events in healthy people and patients with allergies could be obtained.

• Keywords: medicina
• Keywords (english): medicine
• Language: croatian
• URN:NBN: urn:nbn:hr:105:257412
• Study programme: Title: Biomedicine and Health Sciences; Study programme type: university; Study level: postgraduate; Academic / professional title: doktor/doktorica znanosti, područje biomedicine i zdravstva (doktor/doktorica znanosti, područje biomedicine i zdravstva)
• Type of resource: Text
• Extent: 112 str.
• File origin: Born digital
• Access conditions: Open access
• Created on: 2023-05-05 08:41:13