Odnos upalne stanične infiltracije i biljega aktivacije upalnih stanica u bolesnika s kroničnim čeljusnim rinosinuitisom

doctoral thesis

• Title (english): Relation between cellular infiltration and markers in patients with chronic rhinosinusitis
• Author: Davor Vagić; MBZ: 257395
• Mentor: Livije Kalogjera (mentor)
• Committee member: nije dostupno (član povjerenstva)
• Granter: University of Zagreb; School of Medicine; Zagreb
• Defense date and country: 2008-12-30, Croatia
• Scientific / art field, discipline and subdiscipline: BIOMEDICINE AND HEALTHCARE; Clinical Medical Sciences
• Universal decimal classification (UDC): 61 - Medical sciences

Abstract

Istraživanje je proučavalo različite putove upalne reakcije kod ispitanika s kroničnim rinosinuitisom s i bez astme. U istraživanje je bilo uključeno 36 ispitanika s kroničnim rinosinuitisom i 23 ispitanika s kroničnim rinosinuitisom i astmom. Kod svih ispitanika učinjena je sinusoskopija čeljusnih sinusa, ispirak sinusa, biopsija sluznice i mikrobiološki bris. Iz ispirka se određivala koncentracija eozinofilnog kationskog proteina (ECP), mijeloperoksidaze (MPO), triptaze i IgE protutijela. Iz bioptata sluznice brojali su se IgE pozitivne stanice, eozinofili, plazma stanice i limfociti. Upitnik simptoma kroničnog rinosinuitisa ispitanici su ispunjavali na početku istraživanja i nakon 6 mjeseci. ECP, MPO, koncentracija IgE protutijela u ispirku, broj upalnih stanica i IgE pozitivnih stanica značajno su viši u astmatičara. Koncentracija triptaze povezana je samo u astmatičara s koncentracijom IgE protutijela i brojem IgE pozitivnih stanica. U 25 ispitanika s kroničnim rinosinuitisom izolirana je bakterija u sinusu dok je u astmatičara izolirana u 16 ispitanika. Značajna je povezanost ECP-a i MPO-a u bakteriološki pozitivnim sinusima astmatičara. U obje grupe ispitanika zbroj subjektivnih simptoma značajno se smanjuje nakon terapije bez korelacije s objektivnim prametrima upale. Sukladno navedenim rezultatima zaključak je da grupa astmatičara predstavlja posebnu grupu među ispitanicima s kroničnim rinosinuitisom.

Abstract (english)

OBJECTIVES: The aim of the study was to show the difference in the pattern of inflammation (and Th1/Th2 polarization) between asthmatic and non-asthmatic patients with CRS, specifically eosinophil activation, local IgE levels in the sinus fluid and tissue, and the severity of inflammation were measured. ----- METHODS: The maxillary sinus lavages, mucosal biopsies and bacteriological swabs were taken in 23 asthmatic and 36 non-asthmatic adult patients with CRS. The concentrations of IgE, eosinophil cationic protein (ECP), myeloperoxidase (MPO), and tryptase were analyzed and IgE+ cells, eosinophils, lymphocytes and plasma cells counted. ----- RESULTS: The granulocyte activation markers and IgE in sinus lavages, and the inflammatory and IgE + cells counts were significantly higher in the asthmatics with the greatest difference in ECP and IgE concentrations. The tryptase concentrations did not differ, but only in the asthmatics they correlated significantly with the IgE concentrations and IgE+ cells count. Subjective scores were decreased after therapy in both groups of patients and without correlation with objective parameters of inflammation. Conclusion. Asthmatic patients present a distinct subgroup among the patients with chronic rhino sinusitis (CRS). The levels of the cellular markers and Age in the sinus fluid differ from those of non-asthmatic patients with CRS. The activation of granulocytes (especially eosinophils), local IgE concentrations and the inflammatory cells infiltration are significantly higher in the asthmatics.

• Keywords: medicina
• Keywords (english): medicine
• Language: croatian
• URN:NBN: urn:nbn:hr:105:140594
• Study programme: Title: Biomedicine and Health Sciences; Study programme type: university; Study level: postgraduate; Academic / professional title: doktor/doktorica znanosti, područje biomedicine i zdravstva (doktor/doktorica znanosti, područje biomedicine i zdravstva)
• Type of resource: Text
• Extent: 98 str.
• File origin: Born digital
• Access conditions: Open access
• Created on: 2023-05-05 08:51:29