prikaz prve stranice dokumenta Sepsityper® Kit versus In-House Method in Rapid Identification of Bacteria from Positive Blood Cultures by MALDI-TOF Mass Spectrometry
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Scientific paper - Original scientific paper
Sepsityper® Kit versus In-House Method in Rapid Identification of Bacteria from Positive Blood Cultures by MALDI-TOF Mass Spectrometry
Life, 12 (2022), 11; 1744. https://doi.org/10.3390/life12111744
Perše, Gabrijela; Samošćanec, Ivana; Bošnjak, Zrinka; Budimir, Ana; Kuliš, Tomislav; Mareković, Ivana

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Perše, G., Samošćanec, I., Bošnjak, Z., Budimir, A., Kuliš, T. & Mareković, I. (2022). Sepsityper® Kit versus In-House Method in Rapid Identification of Bacteria from Positive Blood Cultures by MALDI-TOF Mass Spectrometry. Life, 12. (11). doi: 10.3390/life12111744

Perše, Gabrijela, et al. "Sepsityper® Kit versus In-House Method in Rapid Identification of Bacteria from Positive Blood Cultures by MALDI-TOF Mass Spectrometry." Life, vol. 12, no. 11, 2022. https://doi.org/10.3390/life12111744

Perše, Gabrijela, Ivana Samošćanec, Zrinka Bošnjak, Ana Budimir, Tomislav Kuliš and Ivana Mareković. "Sepsityper® Kit versus In-House Method in Rapid Identification of Bacteria from Positive Blood Cultures by MALDI-TOF Mass Spectrometry." Life 12, no. 11 (2022). https://doi.org/10.3390/life12111744

Perše, G., et al. (2022) 'Sepsityper® Kit versus In-House Method in Rapid Identification of Bacteria from Positive Blood Cultures by MALDI-TOF Mass Spectrometry', Life, 12(11). doi: 10.3390/life12111744

Perše G, Samošćanec I, Bošnjak Z, Budimir A, Kuliš T, Mareković I. Sepsityper® Kit versus In-House Method in Rapid Identification of Bacteria from Positive Blood Cultures by MALDI-TOF Mass Spectrometry. Life [Internet]. 2022 October 30 [cited 2024 November 30];12(11). doi: 10.3390/life12111744

G. Perše, I. Samošćanec, Z. Bošnjak, A. Budimir, T. Kuliš and I. Mareković, "Sepsityper® Kit versus In-House Method in Rapid Identification of Bacteria from Positive Blood Cultures by MALDI-TOF Mass Spectrometry", Life, vol. 12, no. 11, October 2022. [Online]. Available at: https://urn.nsk.hr/urn:nbn:hr:105:998321. [Accessed: 30 November 2024]

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Metadata
Title (english)Sepsityper® Kit versus In-House Method in Rapid Identification of Bacteria from Positive Blood Cultures by MALDI-TOF Mass Spectrometry
AuthorGabrijela Perše
AuthorIvana Samošćanec
AuthorZrinka Bošnjak
MBZ: 319631
AuthorAna Budimir
AuthorTomislav Kuliš
AuthorIvana Mareković
Author's institutionUniversity of Zagreb
School of Medicine
(Department of Medical Microbiology and Parasitology)
Author's institutionUniversity of Zagreb
School of Medicine
(Department of Urology)
Scientific / art field,
discipline and subdiscipline		BIOMEDICINE AND HEALTHCARE
Clinical Medical Sciences
Urology
Scientific / art field,
discipline and subdiscipline		BIOMEDICINE AND HEALTHCARE
Clinical Medical Sciences
Medical Microbiology
Abstract (english)
In order to further accelerate pathogen identification from positive blood cultures (BC), various sample preparation protocols to identify bacteria with MALDI-TOF MS directly from positive BCs have been developed. We evaluated an in-house method in comparison to the Sepsityper® Kit (Bruker Daltonics, Bremen, Germany) as well as the benefit of an on-plate formic acid extraction step following positive signal by the BACTECTM FX system. Confirmation of identification was achieved using subcultured growing biomass used for MALDI-TOF MS analysis. A total of 113 monomicrobial positive BCs were analyzed. The rates of Gram-positive bacteria correctly identified to the genus level using in-house method and Sepsityper® Kit were 63.3% (38/60) and 81.7% (49/60), respectively (p = 0.025). Identification rates at species level for Gram-positive bacteria with in-house method and Sepsityper® kit were 30.0% (18/60) and 66.7% (40/60), respectively (p < 0.001). Identification rates of Gram-negative bacteria were similar with the in-house method and Sepsityper® Kit. Additional on-plate formic acid extraction demonstrated significant improvement in the identification rate of Gram-positive bacteria at both genus and species level for both in-house (p = 0.001, p < 0.001) and Sepsityper® Kit methods (p = 0.007, p < 0.001). Our in-house method is a candidate for laboratory routines with Sepsityper® Kit as a back-up solution when identification of Gram-positive bacteria is unsuccessful.
Keywords (english)
Languageenglish
Publication typeScientific paper - Original scientific paper
Publication statusPublished
Peer reviewPeer review
Publication versionPublished version
Journal titleLife
Numberingvol. 12, no. 11, 1744
e-ISSN2075-1729
DOIhttps://doi.org/10.3390/life12111744
URN:NBNurn:nbn:hr:105:998321
Publication2022-10-30
Document URLhttps://www.mdpi.com/2075-1729/12/11/1744
Type of resourceText
Access conditionsOpen access
Terms of useLicence Attribution 4.0 International (CC BY 4.0) icon
Created on2023-07-13 06:53:26