Infection with human cytomegalovirus, Epstein-Barr virus, and high-risk types 16 and 18 of human papillomavirus in EGFR-mutated lung adenocarcinoma

Scientific paper - Original scientific paper

• Author: Suzana Harabajsa
• Author: Hajdi Šefčić
• Author: Marija Klasić
• Author: Marija Milavić
• Author: Snježana Židovec Lepej
• Author: Ivana Grgić
• Author: Matea Zajc Petranović
• Author: Marko Jakopović
• Author: Silvana Smojver-Ježek
• Author: Petra Korać
• Author's institution: University of Zagreb; School of Medicine; (Department of Internal Medicine)
• Scientific / art field, discipline and subdiscipline: BIOMEDICINE AND HEALTHCARE; Clinical Medical Sciences; Internal Medicine

Abstract (english)

Aim: To assess the frequency of human cytomegalovirus (HCMV), Epstein-Barr virus (EBV), and high-risk types of human papillomavirus (HPV16 and HPV18) infections in lung adenocarcinoma samples.

Methods: Lung adenocarcinoma cytological smears and their DNA isolates were obtained from patients hospitalized at the Department for Lung Diseases Jordanovac, Zagreb, in 2016 and 2017. Overall, 67 lung adenocarcinoma samples were examined: 34 with epidermal growth factor receptor gene (EGFR) mutations and 33 without EGFR mutations. The EGFR mutation status and virus presence were assessed with a polymerase chain reaction, and random samples were additionally tested for EBV with Sanger sequencing. HCMV, EBV, HPV16, and HPV18 infections were evaluated in relation to EGFR mutation, smoking status, and sex. A meta-analysis of available data about HPV infection in non-small cell lung cancer was performed.

Results: More frequent HCMV, EBV, HPV16, and HPV18 infections were observed in lung adenocarcinoma samples with EGFR mutations than in samples without these mutations. Coinfection of the investigated viruses was observed only in lung adenocarcinoma samples with mutated EGFR. In the group with EGFR mutations, smoking was significantly associated with HPV16 infection. The meta-analysis showed that non-small cell lung cancer patients with EGFR mutations had a higher odds of HPV infection.

Conclusion: HCMV, EBV, and high-risk HPV infections are more frequent in EGFR-mutated lung adenocarcinomas, which indicates a possible viral impact on the etiology of this lung cancer subtype.

• Keywords (english): Adenocarcinoma of Lung / complications; Adenocarcinoma of Lung / genetics; Carcinoma, Non-Small-Cell Lung / complications; Carcinoma, Non-Small-Cell Lung / genetics; Cytomegalovirus / genetics; Epstein-Barr Virus Infections / complications; Epstein-Barr Virus Infections / genetics; ErbB Receptors / genetics; Genes, erbB-1; Herpesvirus 4, Human / genetics; Human Papillomavirus Viruses; Humans; Lung Neoplasms / genetics; Papillomavirus Infections / complications; Papillomavirus Infections / genetics
• Language: english
• Publication type: Scientific paper - Original scientific paper
• Publication status: Published
• Peer review: Peer review
• Publication version: Published version
• Journal title: Croatian Medical Journal
• Numbering: vol. 64, no. 2, pp. 84-92
• p-ISSN: 0353-9504
• e-ISSN: 1332-8166
• DOI: https://doi.org/10.3325/cmj.2023.64.84
• URN:NBN: urn:nbn:hr:105:641754
• Publication: 2023-04
• Document URL: https://www.ncbi.nlm.nih.gov/pmc/articles/PMC10183960/
• Type of resource: Text
• Access conditions: Open access
• Created on: 2023-07-14 09:29:27