Reciprocal Alterations in Osteoprogenitor and Immune Cell Populations in Rheumatoid Synovia

Scientific paper - Original scientific paper

• Author: Katarina Barbarić Starčević
• Author: Nina Lukač
• Author: Mislav Jelić
• Author: Alan Šućur
• Author: Danka Grčević
• Author: Nataša Kovačić
• Author's institution: University of Zagreb; School of Medicine; (Croatian Institiute for Brain Research)
• Author's institution: University of Zagreb; School of Medicine; (Department of Anatomy and Clinical Anatomy)
• Author's institution: University of Zagreb; School of Medicine; (Department of Physiology and Immunology)
• Author's institution: University of Zagreb; School of Medicine; (Department of Orthopedics)
• Scientific / art field, discipline and subdiscipline: BIOMEDICINE AND HEALTHCARE; Basic Medical Sciences; Human Physiology
• Scientific / art field, discipline and subdiscipline: BIOMEDICINE AND HEALTHCARE; Basic Medical Sciences; Anatomy
• Scientific / art field, discipline and subdiscipline: BIOMEDICINE AND HEALTHCARE; Clinical Medical Sciences; Orthopedics
• Scientific / art field, discipline and subdiscipline: BIOMEDICINE AND HEALTHCARE; Basic Medical Sciences; Neuroscience

Abstract (english)

Rheumatoid arthritis (RA) is chronic, autoimmune joint inflammation characterized by irreversible joint destruction. Besides increased resorption, destruction is a result of decreased bone formation, due to suppressed differentiation and function of the mesenchymal lineage-derived osteoblasts in inflammatory milieu. In this study, we analyzed the cellular composition of synovial tissue from 11 RA and 10 control patients harvested during planned surgeries in order to characterize resident synovial progenitor populations. Synovial cells were released by collagenase, and labeled for flow cytometry by two antibody panels: 1. CD3-FITC, CD14-PE, 7-AAD, CD11b-PECy7, CD235a-APC, CD19-APCeF780; and 2. 7-AAD, CD105-PECy7, CD45/CD31/CD235a-APC, and CD200-APCeF780. The proportions of lymphocytes (CD3+, CD19+) and myeloid (CD11b+, CD14+) cells were higher in synovial tissue from the patients with RA than in the controls. Among non-hematopoietic (CD45−CD31−CD235a−) cells, there was a decrease in the proportion of CD200+CD105− and increase in the proportion of CD200−CD105+ cells in synovial tissue from the patients with RA in comparison to the control patients. The proportions of both populations were associated with inflammatory activity and could discriminate between the RA and the controls.

• Keywords (english): Arthritis, Rheumatoid; Flow Cytometry; Fluorescein-5-isothiocyanate; Humans; Synovial Fluid; Synovial Membrane
• Language: english
• Publication type: Scientific paper - Original scientific paper
• Publication status: Published
• Peer review: Peer review
• Publication version: Published version
• Journal title: International Journal of Molecular Sciences
• Numbering: vol. 23, no. 20, 12379
• e-ISSN: 1422-0067
• DOI: https://doi.org/10.3390/ijms232012379
• URN:NBN: urn:nbn:hr:105:727062
• Publication: 2022-10-16
• Document URL: https://www.mdpi.com/1422-0067/23/20/12379
• Type of resource: Text
• Access conditions: Open access
• Created on: 2023-08-21 07:35:10