Povezanost koncentracije endotelne lipaze u serumu s funkcionalnošću HDL čestica i endotelnom disfunkcijom u bolesnika s metaboličkim sindromom

doctoral thesis

• Title (english): Association of endothelial lipase serum levels with functional characteristics of HDL particles and endothelial dysfunction in patients with metabolic syndrome
• Author: Iva Klobučar
• Mentor: Vesna Degoricija (mentor)
• Committee member: Jadranka Sertić (predsjednik povjerenstva)
• Committee member: Darko Kaštelan (član povjerenstva)
• Committee member: Mislav Vrsalović (član povjerenstva)
• Granter: University of Zagreb; School of Medicine; Zagreb
• Defense date and country: 2023-08-30, Croatia
• Scientific / art field, discipline and subdiscipline: BIOMEDICINE AND HEALTHCARE; Clinical Medical Sciences; Internal Medicine
• Universal decimal classification (UDC): 616 - Pathology. Clinical medicine

Abstract

Cilj istraživanja bio je opisati povezanost koncentracije endotelne lipaze u serumu s koncentracijom, sastavom i funkcionalnošću čestica HDL-a te ultrazvučnim pokazateljima funkcije stijenke krvne žile (FMD, NMD) u zdravih ispitanika i ispitanika s metaboličkim sindromom.
U istraživanje je uključeno ukupno 130 ispitanika (65 zdravih i 65 ispitanika s metaboličkim sindromom), prosječne dobi 56 godina (50,0; 60,0), s udjelom žena od 47,7% u svakoj skupini. Koncentracija endotelne lipaze bila je značajno veća; a koncentracija čestica HDL-a, sadržaj fosfolipida i kolesterola prosječnih čestica HDL-a, vrijednosti pokazatelja funkcionalnosti HDL-a te vrijednosti varijabli FMD i NMD bile su značajno manje u ispitanika s metaboličkim sindromom u usporedbi sa zdravima. Unatoč tome, nije pronađena značajna povezanost opisanih varijabli u ispitanika s metaboličkim sindromom. Ipak, u zdravih ispitanika, koncentracija endotelne lipaze je negativno korelirala sa sadržajem fosfolipida u prosječnim česticama HDL-a, a sadržaj fosfolipida i kolesterola u česticama HDL-a velikog promjera negativno je korelirao s varijablom FMD. Dodatno, serumska je koncentracija triglicerida u sastavu velikih čestica HDL-a pozitivno korelirala s varijablama FMD i NMD u zdravih ispitanika.
Zaključno, endotelna lipaza ne predstavlja dominantan modifikator koncentracije, sastava ili funkcionalnosti čestica HDL-a, kao ni funkcije endotela stijenke krvne ţile u uvjetima metaboličkog sindroma. Ipak, u skupini ispitanika nižeg rizika za razvoj kardiovaskularnih bolesti, koji nemaju metabolički sindrom i još uvijek ih smatramo zdravima, koncentracija endotelne lipaze povezana je sa sastavom čestica HDL-a, a sastav čestica HDL-a sličan onome povezanom s koncentracijom endotelne lipaze korelira i s varijablom FMD. Unatoč tome, izravna povezanost koncentracije endotelne lipaze i pokazatelja funkcije endotela stijenke krvne žile nije opisana niti u zdravih ispitanika.

Abstract (english)

The aim of the present study was to determine the associations of endothelial lipase serum levels (EL) with the serum levels, contents and functional characteristics of HDL particles, as well as with the ultrasonographic indicators of blood vessel wall endothelial dysfunction (FMD, NMD) in healthy volunteers (H) and metabolic syndrome patients (MS).
A total of 130 subjects were enrolled in the study (65 H and 65 MS), with a median age of 56 (50.0; 60.0) years and 47.7% of women in each group. EL was significantly higher; while serum levels of HDL particles, their average phospholipid- and cholesterol contents, functional characteristics of HDL, as well as FMD and NMD were significantly lower in MS compared to H. Despite the observed differences, no significant associations of these variables were found in MS. However, in H, EL correlated negatively with the phospholipid content of average HDL particles. Additionally, the phospholipid- and cholesterol contents of the big HDL particles correlated negatively with FMD. Concomitantly, the serum levels of HDL triglycerides were significantly positively associated with FMD and NMD in H.
In conclusion, EL is not a dominant modifier of serum levels of HDL particles, their content or function, nor of the blood vessel wall endothelial function in MS. However, among participants with low cardiovascular risk who are still considered healthy, EL correlates with the HDL particle content; and the HDL particle content, which is similar to the HDL particle content associated with EL, correlates with FMD as well. Still, no significant direct associations of EL with the indicators of blood vessel wall endothelial function were found in H.

• Keywords: endotelna lipaza; HDL; FMD; endotelna disfunkcija; metabolički sindrom
• Keywords (english): endothelial lipase; high-density lipoprotein; flow-mediated dilation; endothelial dysfunction; metabolic syndrome
• Language: croatian
• URN:NBN: urn:nbn:hr:105:417806
• Study programme: Title: Biomedicine and Health Sciences; Study programme type: university; Study level: postgraduate; Academic / professional title: doktor/doktorica znanosti, područje biomedicine i zdravstvo (doktor/doktorica znanosti, područje biomedicine i zdravstvo)
• Type of resource: Text
• Extent: 104 str.
• File origin: Born digital
• Access conditions: Open access
• Created on: 2023-09-05 08:53:03