prikaz prve stranice dokumenta Detection of SARS-CoV-2 Antibodies: Comparison of Enzyme Immunoassay, Surrogate Neutralization and Virus Neutralization Test
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Scientific paper - Original scientific paper
Detection of SARS-CoV-2 Antibodies: Comparison of Enzyme Immunoassay, Surrogate Neutralization and Virus Neutralization Test
Antibodies, 12 (2023), 2; 35. https://doi.org/10.3390/antib12020035
Vilibić-Čavlek, Tatjana; Bogdanić, Maja; Borko, Ema; Hruskar, Zeljka; Zilic, Denis; Ferenc, Thomas; Tabain, Irena; Barbić, Ljubo; Vujica Ferenc, Mateja; Ferenčak, Ivana; Stevanović, Vladimir

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Vilibić Čavlek, T., Bogdanić, M., Borko, E., Hruskar, Z., Zilic, D., Ferenc, T. ... Stevanović, V. (2023). Detection of SARS-CoV-2 Antibodies: Comparison of Enzyme Immunoassay, Surrogate Neutralization and Virus Neutralization Test. Antibodies, 12. (2). doi: 10.3390/antib12020035

Vilibić Čavlek, Tatjana, et al. "Detection of SARS-CoV-2 Antibodies: Comparison of Enzyme Immunoassay, Surrogate Neutralization and Virus Neutralization Test." Antibodies, vol. 12, no. 2, 2023. https://doi.org/10.3390/antib12020035

Vilibić Čavlek, Tatjana, Maja Bogdanić, Ema Borko, Zeljka Hruskar, Denis Zilic, Thomas Ferenc, Irena Tabain, et al. "Detection of SARS-CoV-2 Antibodies: Comparison of Enzyme Immunoassay, Surrogate Neutralization and Virus Neutralization Test." Antibodies 12, no. 2 (2023). https://doi.org/10.3390/antib12020035

Vilibić Čavlek, T., et al. (2023) 'Detection of SARS-CoV-2 Antibodies: Comparison of Enzyme Immunoassay, Surrogate Neutralization and Virus Neutralization Test', Antibodies, 12(2). doi: 10.3390/antib12020035

Vilibić Čavlek T, Bogdanić M, Borko E, Hruskar Z, Zilic D, Ferenc T, and sur.. Detection of SARS-CoV-2 Antibodies: Comparison of Enzyme Immunoassay, Surrogate Neutralization and Virus Neutralization Test. Antibodies [Internet]. 2023 May 10 [cited 2024 April 20];12(2). doi: 10.3390/antib12020035

T. Vilibić Čavlek, et al., "Detection of SARS-CoV-2 Antibodies: Comparison of Enzyme Immunoassay, Surrogate Neutralization and Virus Neutralization Test", Antibodies, vol. 12, no. 2, May 2023. [Online]. Available at: https://urn.nsk.hr/urn:nbn:hr:105:669514. [Accessed: 20 April 2024]

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Metadata
Title (english)Detection of SARS-CoV-2 Antibodies: Comparison of Enzyme Immunoassay, Surrogate Neutralization and Virus Neutralization Test
AuthorTatjana Vilibić-Čavlek
AuthorMaja Bogdanić
AuthorEma Borko
AuthorZeljka Hruskar
AuthorDenis Zilic
AuthorThomas Ferenc
AuthorIrena Tabain
AuthorLjubo Barbić
AuthorMateja Vujica Ferenc
AuthorIvana Ferenčak
AuthorVladimir Stevanović
Author's institutionUniversity of Zagreb
School of Medicine
(Department of Medical Microbiology and Parasitology)
Scientific / art field,
discipline and subdiscipline		BIOMEDICINE AND HEALTHCARE
Clinical Medical Sciences
Medical Microbiology
Abstract (english)
Background: Since sensitivity and specificity vary widely between tests, SARS-CoV-2 serology results should be interpreted with caution. Methods: The study included serum samples from patients who had recovered from COVID-19 (n = 71), individuals vaccinated against SARS-CoV-2 (n = 84), and asymptomatic individuals (n = 33). All samples were tested for the presence of binding antibodies (enzyme immunoassay; EIA), neutralizing (NT) antibodies (virus neutralization test; VNT), and surrogate NT (sNT) antibodies (surrogate virus neutralization test; sVNT) of SARS-CoV-2. Results: SARS-CoV-2-binding antibodies were detected in 71 (100%) COVID-19 patients, 77 (91.6%) vaccinated individuals, and 4 (12.1%) control subjects. Among EIA-positive samples, VNT was positive (titer ≥ 8) in 100% of COVID-19 patients and 63 (75.0%) of the vaccinated individuals, while sVNT was positive (>30% inhibition) in 62 (87.3%) patients and 59 (70.2%) vaccinated individuals. The analysis of antibody levels showed a significant moderate positive correlation between EIA and VNT, a moderate positive correlation between EIA and sVNT, and a strong positive correlation between VNT and sVNT. The proportion of positive sVNT detection rate was associated with VNT titer. The lowest positivity (72.4%/70.8%) was detected in samples with low NT titers (8/16) and increased progressively from 88.2% in samples with titer 32 to 100% in samples with titer 256. Conclusions: sVNT appeared to be a reliable method for the assessment COVID-19 serology in patients with high antibody levels, while false-negative results were frequently observed in patients with low NT titers.
Keywords (english)
Languageenglish
Publication typeScientific paper - Original scientific paper
Publication statusPublished
Peer reviewPeer review
Publication versionPublished version
Journal titleAntibodies
Numberingvol. 12, no. 2, 35
e-ISSN2073-4468
DOIhttps://doi.org/10.3390/antib12020035
URN:NBNurn:nbn:hr:105:669514
Publication2023-05-10
Document URLhttps://www.mdpi.com/2073-4468/12/2/35
Type of resourceText
Access conditionsOpen access
Terms of useLicence Attribution 4.0 International (CC BY 4.0) icon
Created on2023-10-06 08:10:11