Methylation of Immune Gene Promoters in Oral and Oropharyngeal Cancer

Scientific paper - Original scientific paper

• Author: Petra Anić
• Author: Jasminka Golubić Talić
• Author: Ksenija Božinović
• Author: Emil Dediol
• Author: Marinka Mravak-Stipetić
• Author: Magdalena Grce
• Author: Nina Milutin Gašperov
• Author's institution: University of Zagreb; School of Medicine; (Department of Maxillofacial Surgery and Dentistry)
• Scientific / art field, discipline and subdiscipline: BIOMEDICINE AND HEALTHCARE; Clinical Medical Sciences; Surgery

Abstract (english)

The proportion of oral and oropharyngeal squamous cell carcinoma (OOSCC) that can be attributed to human papillomavirus (HPV) infection is growing nowadays. A potential factor indicating the occurrence of HPV-positive OSCC is a change in the degree of methylation of gene promoters that play a key role in the immune response. In this study, we investigated the difference in the methylation of EDARADD, GBP4, HAVCR2, HLA DPB1, IL12RB1, MARCO, and SIGLEC12 gene promoters in samples of healthy oral mucosa versus samples of oral and oropharyngeal cancer. The presence of HPV infection in samples was examined earlier. To determine the difference in methylation of those gene promotors, isolated and bisulfite-modified DNA was analysed by the methylation-specific PCR method. The investigated gene promoters were found to be more hypomethylated in the oral and oropharyngeal cancer samples in comparison to normal tissue. The proportion of unmethylated gene promoters was similar in HPV-positive and HPV-negative cancers, although the data should be confirmed on a larger set of samples. To conclude, in samples of healthy oral mucosa, the investigated gene promoters were found to be methylated in a high percentage (73.3% to 100%), while in oral and oropharyngeal cancer samples, they were methylated in a low percentage (11.1% to 37%), regardless of HPV infection.

• Keywords (english): Carcinoma, Squamous Cell / pathology; DNA Methylation; Head and Neck Neoplasms / genetics; Humans; Mouth Neoplasms / genetics; Mouth Neoplasms / pathology; Oropharyngeal Neoplasms / genetics; Oropharyngeal Neoplasms / pathology; Papillomaviridae / genetics; Papillomavirus Infections / complications; Papillomavirus Infections / epidemiology; Papillomavirus Infections / genetics; Squamous Cell Carcinoma of Head and Neck / genetics
• Language: english
• Publication type: Scientific paper - Original scientific paper
• Publication status: Published
• Peer review: Peer review
• Publication version: Published version
• Journal title: International Journal of Molecular Sciences
• Numbering: vol. 24, no. 9, 7698
• e-ISSN: 1422-0067
• DOI: https://doi.org/10.3390/ijms24097698
• URN:NBN: urn:nbn:hr:105:609986
• Publication: 2023-04-22
• Project: Number: IP-2013-11-4758; Title (croatian): Epigenetičke promjene karcinoma pločastih stanica glave i vrata; Title (english): Epigenetic changes in head and neck squamous cell carcinoma; Acronym: EpiC-HNSCC; Leader: Magdalena Grce; Jurisdiction: Croatia; Funding stream: IP
• Document URL: https://www.mdpi.com/1422-0067/24/9/7698
• Type of resource: Text
• Access conditions: Open access
• Created on: 2023-11-02 09:19:38