prikaz prve stranice dokumenta Association of Cognitive Deficit with Glutamate and Insulin Signaling in a Rat Model of Parkinson’s Disease
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Scientific paper - Original scientific paper
Association of Cognitive Deficit with Glutamate and Insulin Signaling in a Rat Model of Parkinson’s Disease
Biomedicines, 11 (2023), 3; 683. https://doi.org/10.3390/biomedicines11030683
Knezović, Ana; Piknjac, Marija; Osmanović Barilar, Jelena; Babić Perhoč, Ana; Virag, Davor; Homolak, Jan; Šalković-Petrišić, Melita

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Knezović, A., Piknjac, M., Osmanović Barilar, J., Babić Perhoč, A., Virag, D., Homolak, J. & Šalković Petrišić, M. (2023). Association of Cognitive Deficit with Glutamate and Insulin Signaling in a Rat Model of Parkinson’s Disease. Biomedicines, 11. (3). doi: 10.3390/biomedicines11030683

Knezović, Ana, et al. "Association of Cognitive Deficit with Glutamate and Insulin Signaling in a Rat Model of Parkinson’s Disease." Biomedicines, vol. 11, no. 3, 2023. https://doi.org/10.3390/biomedicines11030683

Knezović, Ana, Marija Piknjac, Jelena Osmanović Barilar, Ana Babić Perhoč, Davor Virag, Jan Homolak and Melita Šalković Petrišić. "Association of Cognitive Deficit with Glutamate and Insulin Signaling in a Rat Model of Parkinson’s Disease." Biomedicines 11, no. 3 (2023). https://doi.org/10.3390/biomedicines11030683

Knezović, A., et al. (2023) 'Association of Cognitive Deficit with Glutamate and Insulin Signaling in a Rat Model of Parkinson’s Disease', Biomedicines, 11(3). doi: 10.3390/biomedicines11030683

Knezović A, Piknjac M, Osmanović Barilar J, Babić Perhoč A, Virag D, Homolak J, and sur.. Association of Cognitive Deficit with Glutamate and Insulin Signaling in a Rat Model of Parkinson’s Disease. Biomedicines [Internet]. 2023 February 23 [cited 2024 November 10];11(3). doi: 10.3390/biomedicines11030683

A. Knezović, et al., "Association of Cognitive Deficit with Glutamate and Insulin Signaling in a Rat Model of Parkinson’s Disease", Biomedicines, vol. 11, no. 3, February 2023. [Online]. Available at: https://urn.nsk.hr/urn:nbn:hr:105:722961. [Accessed: 10 November 2024]

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Metadata
Title (english)Association of Cognitive Deficit with Glutamate and Insulin Signaling in a Rat Model of Parkinson’s Disease
AuthorAna Knezović
AuthorMarija Piknjac
AuthorJelena Osmanović Barilar
AuthorAna Babić Perhoč
AuthorDavor Virag
AuthorJan Homolak
AuthorMelita Šalković-Petrišić
Author's institutionUniversity of Zagreb
School of Medicine
(Department of Pharmacology)
Scientific / art field,
discipline and subdiscipline		BIOMEDICINE AND HEALTHCARE
Basic Medical Sciences
Pharmacology
Abstract (english)
Cognitive deficit is a frequent non-motor symptom in Parkinson's disease (PD) with an unclear pathogenesis. Recent research indicates possible involvement of insulin resistance and glutamate excitotoxicity in PD development. We investigated cognitive performance and the brain glutamate and insulin signaling in a rat model of PD induced by bilateral intrastriatal injection of 6-hydroxydopamine (6-OHDA). Cognitive functions were assessed with Passive Avoidance (PA) and Morris Water Maze (MWM) tests. The expression of tyrosine hydroxylase (TH) and proteins involved in insulin (insulin receptor - IR, phosphoinositide 3 kinase - pI3K, extracellular signal-regulated kinases-ERK) and glutamate receptor (α-amino-3-hydroxy-5-methyl-4-isoxazolepropionic acid receptos-AMPAR, N-methyl-D-aspartate receptor - NMDAR) signaling was assessed in the hippocampus (HPC), hypothalamus (HPT) and striatum (S) by immunofluorescence, Western blot and enzyme-linked immunosorbent assay (ELISA). Three months after 6-OHDA treatment, cognitive deficit was accompanied by decreased AMPAR activity and TH levels (HPC, S), while levels of the proteins involved in insulin signaling remained largely unchanged. Spearman's rank correlation revealed a strong positive correlation for pAMPAR-PA (S), pNMDAR-pI3K (HPC) and pNMDAR-IR (all regions). Additionally, a positive correlation was found for TH-ERK and TH-pI3K, and a negative one for TH-MWM/errors and pI3K-MWM/time (S). These results suggest a possible association between brain glutamate (but not insulin) signaling dysfunction and cognitive deficit in a rat PD model, detected three months after 6-OHDA treatment.
Keywords (english)
Languageenglish
Publication typeScientific paper - Original scientific paper
Publication statusPublished
Peer reviewPeer review
Publication versionPublished version
Journal titleBiomedicines
Numberingvol. 11, no. 3, 683
e-ISSN2227-9059
DOIhttps://doi.org/10.3390/biomedicines11030683
URN:NBNurn:nbn:hr:105:722961
Publication2023-02-23
ProjectNumber: IP-2018-01-8938
Title (croatian): Mehanizmi nutrijentom posredovanih učinaka endogenog glukagonu sličnog peptida-1 na kognitivne i metaboličke poremećaje u eksperimentalnim modelima neurodegenerativnih bolesti
Acronym: NutrientGLP-1
Leader: Melita Šalković-Petrišić
Jurisdiction: Croatia
Funding stream: IP
Document URLhttps://www.mdpi.com/2227-9059/11/3/683
Type of resourceText
Access conditionsOpen access
Terms of useLicence Attribution 4.0 International (CC BY 4.0) icon
Created on2024-01-08 13:21:23