Botulinum toxin's axonal transport from periphery to the spinal cord

Scientific paper - Original scientific paper

• Author: Ivica Matak
• Author: Peter Riederer
• Author: Zdravko Lacković
• Author's institution: University of Zagreb; School of Medicine
• Scientific / art field, discipline and subdiscipline: BIOMEDICINE AND HEALTHCARE; Basic Medical Sciences

Abstract (english)

Axonal transport of enzymatically active botulinum toxin A (BTX-A) from periphery to the CNS has been described in facial and trigeminal nerve, leading to cleavage of synaptosomal-associated protein 25 (SNAP-25) in central nuclei. Aim of present study was to examine the existence of axonal transport of peripherally applied BTX-A to spinal cord via sciatic nerve. We employed BTX-A-cleaved SNAP-25 immunohistochemistry of lumbar spinal cord after intramuscular and subcutaneous hind limb injections, and intraneural BTX-A sciatic nerve injections. Truncated SNAP-25 in ipsilateral spinal cord ventral horns and dorsal horns appeared after single peripheral BTX-A administrations, even at low intramuscular dose applied (5 U/kg). Cleaved SNAP-25 appearance in the spinal cord after BTX-A injection into the sciatic nerve was prevented by proximal intrasciatic injection of colchicine (5 mM, 2 μl). Cleaved SNAP-25 in ventral horn, using choline-acetyltransferase (ChAT) double labeling, was localized within cholinergic neurons. These results extend the recent findings on BTX-A retrograde axonal transport in facial and trigeminal nerve. Appearance of truncated SNAP-25 in spinal cord following low-dose peripheral BTX-A suggest that the axonal transport of BTX-A occurs commonly following peripheral application.

• Keywords (english): Animals; Axonal Transport/physiology; Botulinum Toxins, Type A/administration & dosage; Botulinum Toxins, Type A/metabolism; Choline O-Acetyltransferase/metabolism; Colchicine/pharmacology; Hindlimb/physiology; Immunohistochemistry; Injections; Injections, Intramuscular; Injections, Subcutaneous; Male; Microscopy, Confocal; Motor Neurons/metabolism; Peripheral Nerves/metabolism; Rats; Rats, Wistar; Sciatic Nerve/metabolism; Sensory Receptor Cells/metabolism; Spinal Cord/metabolism; Synaptosomal-Associated Protein 25/metabolism
• Language: english
• Publication type: Scientific paper - Original scientific paper
• Publication status: Published
• Peer review: Peer review
• Publication version: Accepted version
• Journal title: Neurochemistry International
• Numbering: vol. 61, no. 2, pp. 236-239
• p-ISSN: 0197-0186
• DOI: https://doi.org/10.1016/j.neuint.2012.05.001
• URN:NBN: urn:nbn:hr:105:651272
• Publication: 2012-07
• Document URL: https://doi.org/10.1016/j.neuint.2012.05.001
• Type of resource: Text
• Access conditions: Open access
• Created on: 2024-03-27 19:34:56