Pseudocholinesterase activity in cerebrospinal fluid as a biomarker of solid central nervous system tumors in children

Scientific paper - Original scientific paper

• Author: Lili Mikecin
• Author: Miljenko Križmarić
• Author: Jasminka Stepan Giljević
• Author: Miroslav Gjurašin
• Author: Josipa Kern
• Author: Jasna Leniček Krleža
• Author: Ljiljana Popović
• Author's institution: University of Zagreb; School of Medicine
• Scientific / art field, discipline and subdiscipline: BIOMEDICINE AND HEALTHCARE; Clinical Medical Sciences

Abstract (english)

AIM:

To determine the activity of pseudocholinesterase (PChE) in cerebrospinal fluid (CSF) and serum in children with solid central nervous system (CNS) tumor and to assess whether PChE activity could be a valid biomarker for solid CNS tumors in children. ----- METHODS:

The study and control group included 30 children each. Children in the study group had a solid CNS tumor, while those from the control group had never suffered from any tumor diseases. CSF and serum samples were collected from all participants and PChE activity was determined using the Ellman's spectrophotometric method. PChE activity in CSF was shown as a cerebrospinal fluid/serum ratio expressed in percentage, ie, PChE CSF/serum ratio. Receiver operating characteristic (ROC) curve was used to assess whether PChE activity can be used as a biomarker for identifying children with solid CNS tumors. ----- RESULTS:

Children with solid CNS tumor had significantly higher PChE activity in CSF and serum, as well as PChE CSF/serum ratio (P=0.001). PChE CSF/serum ratio in the study group was 2.38% (interquartile range [IQR] 1.14-3.97) and 1.09% (IQR 0.95-1.45) in the control group. ROC curve analysis of PChE CSF/serum ratio resulted in an area under the curve (AUC) value of 0.76 (95% confidence interval [CI] 0.63-0.88) and a cut-off of 1.09. Twenty five of 29 patients with elevated PChE CSF/serum ratio had a tumor, corresponding to a sensitivity of 83% and a specificity of 53%. ----- CONCLUSION:

PChE CSF/serum ratio may be used as a test or biomarker with good sensitivity for solid CNS tumors in children.

• Keywords (english): Adolescent; Case-Control Studies; Central Nervous System Neoplasms/blood; Central Nervous System Neoplasms/cerebrospinal fluid; Central Nervous System Neoplasms/diagnosis; Child; Child, Preschool; Female; Humans; Male; Pseudocholinesterase/blood; Pseudocholinesterase/cerebrospinal fluid; ROC Curve; Sensitivity and Specificity; Tumor Markers, Biological/blood; Tumor Markers, Biological/cerebrospinal fluid
• Language: english
• Publication type: Scientific paper - Original scientific paper
• Publication status: Published
• Peer review: Peer review
• Publication version: Published version
• Journal title: Croatian Medical Journal
• Numbering: vol. 54, no. 5, pp. 429-435
• p-ISSN: 0353-9504
• DOI: https://doi.org/10.3325/cmj.2013.54.429
• URN:NBN: urn:nbn:hr:105:396089
• Publication: 2013-10-28
• Document URL: https://doi.org/10.3325/cmj.2013.54.429
• Type of resource: Text
• Access conditions: Open access
• Created on: 2024-03-27 20:08:02