prikaz prve stranice dokumenta Exogenous heparin binds and inhibits bone morphogenetic protein 6 biological activity
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Scientific paper - Original scientific paper
Exogenous heparin binds and inhibits bone morphogenetic protein 6 biological activity
International Orthopaedics, 37 (2013), 3; 529-541. https://doi.org/10.1007/s00264-012-1714-3
Brkljačić, Jelena; Pauk, Martina; Erjavec, Igor; Cipčić, Antonio; Grgurević, Lovorka; Zadro, Renata; Inman, Gareth J.; Vukičević, Slobodan

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Brkljačić, J., Pauk, M., Erjavec, I., Cipčić, A., Grgurević, L., Zadro, R. ... Vukičević, S. (2013). Exogenous heparin binds and inhibits bone morphogenetic protein 6 biological activity. International Orthopaedics, 37. (3), 529-541. doi: 10.1007/s00264-012-1714-3

Brkljačić, Jelena, et al. "Exogenous heparin binds and inhibits bone morphogenetic protein 6 biological activity." International Orthopaedics, vol. 37, no. 3, 2013, pp. 529-541. https://doi.org/10.1007/s00264-012-1714-3

Brkljačić, Jelena, Martina Pauk, Igor Erjavec, Antonio Cipčić, Lovorka Grgurević, Renata Zadro, Gareth J. Inman and Slobodan Vukičević. "Exogenous heparin binds and inhibits bone morphogenetic protein 6 biological activity." International Orthopaedics 37, no. 3 (2013): 529-541. https://doi.org/10.1007/s00264-012-1714-3

Brkljačić, J., et al. (2013) 'Exogenous heparin binds and inhibits bone morphogenetic protein 6 biological activity', International Orthopaedics, 37(3), pp. 529-541. doi: 10.1007/s00264-012-1714-3

Brkljačić J, Pauk M, Erjavec I, Cipčić A, Grgurević L, Zadro R, and sur.. Exogenous heparin binds and inhibits bone morphogenetic protein 6 biological activity. International Orthopaedics [Internet]. 2013 March [cited 2024 December 20];37(3):529-541. doi: 10.1007/s00264-012-1714-3

J. Brkljačić, et al., "Exogenous heparin binds and inhibits bone morphogenetic protein 6 biological activity", International Orthopaedics, vol. 37, no. 3, pp. 529-541, March 2013. [Online]. Available at: https://urn.nsk.hr/urn:nbn:hr:105:842682. [Accessed: 20 December 2024]

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Metadata
Title (english)Exogenous heparin binds and inhibits bone morphogenetic protein 6 biological activity
AuthorJelena Brkljačić
AuthorMartina Pauk
AuthorIgor Erjavec
AuthorAntonio Cipčić
AuthorLovorka Grgurević
AuthorRenata Zadro
AuthorGareth J. Inman
AuthorSlobodan Vukičević
Author's institutionUniversity of Zagreb
School of Medicine
Scientific / art field,
discipline and subdiscipline		BIOMEDICINE AND HEALTHCARE
Basic Medical Sciences
Abstract (english)
PURPOSE: The purpose of this study was to explore the effect of heparin on bone morphogenetic protein 6 (BMP6) osteogenic activity. ----- METHODS: Western blot analysis was used to confirm the binding of BMP6 to heparin and to observe its effect on BMP6 signaling in C2C12-BRE-Luc myoblasts. Real-time RT-PCR was performed for the expression analysis of alkaline phosphatase (ALP) and osteocalcin (OC) in C2C12 myoblasts treated with BMP6 and heparin for 72 hours. Rat ectopic bone formation assay was performed to explore the effect of heparin on BMP6 osteogenic activity. Two weeks following implantation the implants were analysed morphologically and histologically. A mouse osteoporotic model was used to test the ability of BMP6 to improve the bone quality in vivo in the presence of heparin, followed by DEXA and μCT analyses. Blood coagulation was tested in rats previously treated with BMP6. ----- RESULTS: BMP6 specifically bound to heparin and induced Smad1/5/8 phosphorylation which was inhibited by heparin. After 48 and 72 hours of treatment, heparin inhibited BMP6-induced ALP and OC expression in C2C12 cells. Heparin dose dependently inhibited BMP6-induced new bone and cartilage formation in the rat ectopic bone formation assay, while in osteoporotic mice heparin inhibited the BMP6 potential to improve the bone quality as evidenced by decreased bone mineral density and trabecular bone parameters. Interestingly, BMP6 prevented the effect of heparin on the blood coagulation parameters. ----- CONCLUSION: The interaction of BMP6 with heparin might contribute to the heparin-induced osteoporosis and blood coagulation.
Keywords (english)
Languageenglish
Publication typeScientific paper - Original scientific paper
Publication statusPublished
Peer reviewPeer review
Publication versionAccepted version
Journal titleInternational Orthopaedics
Numberingvol. 37, no. 3, pp. 529-541
p-ISSN0341-2695
DOIhttps://doi.org/10.1007/s00264-012-1714-3
URN:NBNurn:nbn:hr:105:842682
Publication2013-03
Document URLhttp://doi.org/10.1007/s00264-012-1714-3
Type of resourceText
Access conditionsOpen access
Terms of useLicence In copyright icon
Created on2024-03-28 08:09:19