Procjena embriotoksičnosti valproata iz infracrvenih spektara metaboloma medija tijekom kulture štakorskog zametka

master's thesis

• Title (english): Assessment of valproate embriotoxicity from infrared spectra of ebrio culture media metabolome
• Author: Vedran Radonić
• Mentor: Maja Balarin (mentor)
• Committee member: Sanja Dolanski-Babić (predsjednik povjerenstva)
• Committee member: Ozren Gamulin (član povjerenstva)
• Committee member: Maja Balarin (član povjerenstva)
• Granter: University of Zagreb; School of Medicine; (Department of Physics and Biophysics); Zagreb
• Defense date and country: 2015-07-15, Croatia
• Scientific / art field, discipline and subdiscipline: BIOMEDICINE AND HEALTHCARE; Basic Medical Sciences; Medical Biochemistry

Abstract

Uzorci medija u kojima su rasli štakorski zameci prikupljani su na Zavodu za biologiju i pohranjivani za daljnju analizu. FTIR spektroskopijom snimljeni su spektri metaboloma medija s i bez valproata u kojima su 14 dana rasli štakorski zameci. Spektri su analizirani statističkim metodama PCA i PC regresije. Pokazalo se da metoda FTIR spektroskopije može razlikovati metabolom medija kontrole u kojem su rasli štakorski zameci bez valproata od onog s valproatom. Pored toga utvrdili smo kako spektroskopska analiza metaboloma medija sadrži podatak o razlici u fazi rasta i razvoja zametaka kroz promatrani period vremena te smo pokazali da se iz spektara medija može odrediti koliko dugo su zameci rasli u kulturi. Rezultati ovog rada ukazuju da FTIR spektroskopska analiza metaboloma medija može biti novi model za probir različitih embriotoksičnih i teratogenih supstanci.

Abstract (english)

Samples of the media in which rat embryos grew, were collected at the Department of Biology and stored for further analysis. Using FTIR spectroscopy the spectra of media metabolome in which rat embryos grew for 14 days, with and without valproate, were recorded. The spectra were analyzed by statistical methods of PCA and PC regression. It was shown that the method of FTIR spectroscopy can distinguish media metabolome between control group in which rat embryos grew without valproate and the one in which they grew with valproate. We have established that the spectroscopic analysis of the metabolome media contains information about the difference in the growth and development of embryos through the monitored period of time. Using PCA analysis we have shown that from metabolome spectra we can determine for how long the embryos grow in culture. These results suggest that FTIR spectroscopic analysis of the media metabolome can be a new model for the screening of different embryo toxic and teratogenic substances.

• Keywords: FTIR; metabolom; valproat; PCA
• Keywords (english): FTIR; metabolome; valproate; PCA
• Language: croatian
• URN:NBN: urn:nbn:hr:105:352702
• Study programme: Title: Medicine; Study programme type: university; Study level: integrated undergraduate and graduate; Academic / professional title: doktor/doktorica medicine (doktor/doktorica medicine)
• Type of resource: Text
• File origin: Born digital
• Access conditions: Open access
• Created on: 2016-08-09 07:04:52