prikaz prve stranice dokumenta Combined metformin and insulin treatment reverses metabolically impaired omental adipogenesis and accumulation of 4-hydroxynonenal in obese diabetic patients
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Scientific paper - Original scientific paper
Combined metformin and insulin treatment reverses metabolically impaired omental adipogenesis and accumulation of 4-hydroxynonenal in obese diabetic patients
Redox Biology, 12 (2017); 483-490. https://doi.org/10.1016/j.redox.2017.03.012
Jaganjac, Morana; Almuraikhy, Shamma; Al-Khelaifi, Fatima; Al-Jaber, Mashael; Bashah, Moataz; Mazloum, Nayef A.; Žarković, Kamelija; Žarković, Neven; Waeg, Georg; Kafienah, Wael; Elrayess, Mohamed A.

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Jaganjac, M., Almuraikhy, S., Al Khelaifi, F., Al Jaber, M., Bashah, M., Mazloum, N. A. ... Elrayess, M. A. (2017). Combined metformin and insulin treatment reverses metabolically impaired omental adipogenesis and accumulation of 4-hydroxynonenal in obese diabetic patients. Redox Biology, 12., 483-490. doi: 10.1016/j.redox.2017.03.012

Jaganjac, Morana, et al. "Combined metformin and insulin treatment reverses metabolically impaired omental adipogenesis and accumulation of 4-hydroxynonenal in obese diabetic patients." Redox Biology, vol. 12, 2017, pp. 483-490. https://doi.org/10.1016/j.redox.2017.03.012

Jaganjac, Morana, Shamma Almuraikhy, Fatima Al Khelaifi, Mashael Al Jaber, Moataz Bashah, Nayef A. Mazloum, Kamelija Žarković, et al. "Combined metformin and insulin treatment reverses metabolically impaired omental adipogenesis and accumulation of 4-hydroxynonenal in obese diabetic patients." Redox Biology 12 (2017): 483-490. https://doi.org/10.1016/j.redox.2017.03.012

Jaganjac, M., et al. (2017) 'Combined metformin and insulin treatment reverses metabolically impaired omental adipogenesis and accumulation of 4-hydroxynonenal in obese diabetic patients', Redox Biology, 12, pp. 483-490. doi: 10.1016/j.redox.2017.03.012

Jaganjac M, Almuraikhy S, Al Khelaifi F, Al Jaber M, Bashah M, Mazloum NA, and sur.. Combined metformin and insulin treatment reverses metabolically impaired omental adipogenesis and accumulation of 4-hydroxynonenal in obese diabetic patients. Redox Biology [Internet]. 2017 August [cited 2025 April 05];12:483-490. doi: 10.1016/j.redox.2017.03.012

M. Jaganjac, et al., "Combined metformin and insulin treatment reverses metabolically impaired omental adipogenesis and accumulation of 4-hydroxynonenal in obese diabetic patients", Redox Biology, vol. 12, pp. 483-490, August 2017. [Online]. Available at: https://urn.nsk.hr/urn:nbn:hr:105:491465. [Accessed: 05 April 2025]

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Metadata
Title (english)Combined metformin and insulin treatment reverses metabolically impaired omental adipogenesis and accumulation of 4-hydroxynonenal in obese diabetic patients
AuthorMorana Jaganjac
AuthorShamma Almuraikhy
AuthorFatima Al-Khelaifi
AuthorMashael Al-Jaber
AuthorMoataz Bashah
AuthorNayef A. Mazloum
AuthorKamelija Žarković
AuthorNeven Žarković
AuthorGeorg Waeg
AuthorWael Kafienah
AuthorMohamed A. Elrayess
Author's institutionUniversity of Zagreb
School of Medicine
Scientific / art field,
discipline and subdiscipline		BIOMEDICINE AND HEALTHCARE
Clinical Medical Sciences
Abstract (english)
OBJECTIVE: Obesity-associated impaired fat accumulation in the visceral adipose tissue can lead to ectopic fat deposition and increased risk of insulin resistance and type 2 diabetes mellitus (T2DM). This study investigated whether impaired adipogenesis of omental (OM) adipose tissues and elevated 4-hydroxynonenal (4-HNE) accumulation contribute to this process, and if combined metformin and insulin treatment in T2DM patients could rescue this phenotype. ----- METHODS: OM adipose tissues were obtained from forty clinically well characterized obese individuals during weight reduction surgery. Levels of 4-HNE protein adducts, adipocyte size and number of macrophages were determined within these tissues by immunohistochemistry. Adipogenic capacity and gene expression profiles were assessed in preadipocytes derived from these tissues in relation to insulin resistance and in response to 4-HNE, metformin or combined metformin and insulin treatment. ----- RESULTS: Preadipocytes isolated from insulin resistant (IR) and T2DM individuals exhibited lower adipogenesis, marked by upregulation of anti-adipogenic genes, compared to preadipocytes derived from insulin sensitive (IS) individuals. Impaired adipogenesis was also associated with increased 4-HNE levels, smaller adipocytes and greater macrophage presence in the adipose tissues. Within the T2DM group, preadipocytes from combined metformin and insulin treated subset showed better in vitro adipogenesis compared to metformin alone, which was associated with less presence of macrophages and 4-HNE in the adipose tissues. Treatment of preadipocytes in vitro with 4-HNE reduced their adipogenesis and increased proliferation, even in the presence of metformin, which was partially rescued by the presence of insulin. ----- CONCLUSION: This study reveals involvement of 4-HNE in the impaired OM adipogenesis-associated with insulin resistance and T2DM and provides a proof of concept that this impairment can be reversed by the synergistic action of insulin and metformin. Further studies are needed to evaluate involvement of 4-HNE in metabolically impaired abdominal adipogenesis and to confirm benefits of combined metformin-insulin therapy in T2DM patients.
Languageenglish
Publication typeScientific paper - Original scientific paper
Publication statusPublished
Peer reviewPeer review
Publication versionPublished version
Journal titleRedox Biology
Numberingvol. 12, pp. 483-490
p-ISSN2213-2317
DOIhttps://doi.org/10.1016/j.redox.2017.03.012
URN:NBNurn:nbn:hr:105:491465
Publication2017-08
Document URLhttps://doi.org/10.1016/j.redox.2017.03.012
Type of resourceText
Access conditionsOpen access
Terms of useLicence In copyright icon
Created on2024-03-28 09:07:06