| Abstract (english) | The aim of the study was to investigate the molecular epidemiology of community- associated MRSA in Primorsko-Goranska County of Croatia during a six-year period(2001-2007). In period from 2001 and 2007, 46 MRSA isolates were collected in Rijeka, strains were subjected to susceptibility testing according to CLSI guidelines, mecA gene detection and SCCmec typing as well as detection of PVL. Strains were typed by Pulse Field Gel Electrophoresis (PFGE) and spa typing. All isolates were susceptible to vancomycin, linezolid, mupirocin, nitrofurantoin, only one strain was resistant to fusidic acid and co-trimoxazole. Results of SCCmec typing showed the presence of SCCmec type IV in 26 MRSA strains, SCCmec type V in three strains, and 13 strains comprised SCCmec I. SCCmec type II and III were not observed. Four MRSA strains were non-typeable by applied SCCmec typing methods. PVL was detected in 4 strains, two SCCmec IV and two SCCmec V. PFGE analysis, grouped MRSA strains into six similarity groups and 18 singletons. Dominating spa types in this collection of strains were t015, with 15 strains, followed by t041(N=7), t051,(N=2 ), t2850(N=2), t008(N=2)and single isolates t441, t002, t448, t018, t019, t355, t390, t026, t449, t148. We also detected two new spa types, t3510 and t3509, respectively. This is the first report on SCCmec type V in Croatia, and, to our knowledge, first report of PVL-positive mehicillin-resistant Staphylococcus aureus SCCmec type V and t441(ST59-MRSA-V) in this part of Europe. |
| Abstract (croatian) | Cilj studije je bio istražiti epidemiološke i molekulne karakteristika MRSA izolata iz Primorsko-Goranske županije tijekom šestogodišnjeg perioda. U periodu od 2001. Do 2007. godine, 46 MRSA izolata je prikupljeno, testirana je osjetljivost na antimikrobne lijekove prema CLSI smjernicama, također je provedena detekcija mecA gena, SCCmec tipizacija i detekcija PVL kodirajućih gena. Tipizacija sojeva dodatno je upotpunjena analizom elektroforeze u pulsirajućem polju (PFGE analizom) i tipizacijom spa lokusa. Svi MRSA izolati su osjetljivi na vankomicin, linezolid, mupirocin, nitrofurantoin, samo jedan soj je bio rezistentan na fucidinsku kiselinu i ko-trimoksazol. Rezultati SCCmec tipizacije pokazali su prisutnost SCCmec tipa IV u 26 MRSA sojeva, SCCmec tipa V kod tri izolata, a 13 sojeva je imalo SCCmec I. SCCmec tipovi II i III nisu nađeni. Četiri MRSA su bila netipabilna navedenom metodom. PVL je detektiran kod 4 soja, dva SCCmec IV I dva SCCmec V. PFGE analiza grupirala je MRSA sojeve u 6 grupa sličnosti 18 pojedinačnih, nesvrstanih sojeva. Dominantni spa tipovi u ovoj kolekciji sojeva su bili: t015, s 15 sojeva, te t041 (N=7), t051, (N=2 ), t2850 (N=2), t008 (N=2) i s po jednim izolatom tipiziranim kao: t441, t002, t448, t018, t019, t355, t390, t026, t449, t148. Otkrili smo i nove tipove, t3510 i t3509. Ovo je prvi spomen o prisutnosti SCCmec tipa V u Hrvatskoj, i, prema našem saznanju, prvo izvješće od PVL-pozitivnom MRSA s SCCmec tipom V i spa tipom t441 (ST59-MRSA-V) u ovom dijelu Europe. |
