Counteraction of perforated cecum lesions in rats: effects of pentadecapeptide BPC 157, L-NAME and L-arginine

Scientific paper - Original scientific paper

• Author: Domagoj Drmić
• Author: Mariam Samara
• Author: Tinka Vidović
• Author: Dominik Malekinušić
• Author: Marko Antunović
• Author: Borna Vrdoljak
• Author: Jelena Ruzman
• Author: Marija Milković Periša
• Author: Katarina Horvat Pavlov
• Author: Jerusha Jeyakumar
• Author: Sven Seiwerth
• Author: Predrag Sikirić
• Author's institution: University of Zagreb; School of Medicine
• Scientific / art field, discipline and subdiscipline: BIOMEDICINE AND HEALTHCARE; Clinical Medical Sciences

Abstract (english)

AIM:

To study the counteraction of perforated cecum lesion using BPC 157 and nitric oxide (NO) system agents. -----

METHODS:

Alongside with the agents' application (after 1 min, medication (/kg, 10 mL/2 min bath/rat) includes: BPC 157 (10 μg), L-NAME (5 mg), L-arginine (100mg) alone or combined, and saline baths (controls)) on the rat perforate cecum injury, we continuously assessed the gross reappearance of the vessels (USB microcamera) quickly propagating toward the defect at the cecum surface, defect contraction, bleeding attenuation, MDA- and NO-levels in cecum tissue at 15 min, and severity of cecum lesions and adhesions at 1 and 7 d. -----

RESULTS:

Post-injury, during/after a saline bath, the number of vessels was significantly reduced, the defect was slightly narrowed, bleeding was significant and MDA-levels increased and NO-levels decreased. BPC 157 bath: the vessel presentation was markedly increased, the defect was noticeably narrowed, the bleeding time was shortened and MDA- and NO-levels remained normal. L-NAME: reduced vessel presentation but not more than the control, did not change defect and shortened bleeding. L-arginine: exhibited less vessel reduction, did not change the defect and prolonged bleeding. In combination, mutual counteraction occurred (L-NAME + L-arginine) or the presentation was similar to that of BPC 157 rats (BPC 157 + L-NAME; BPC 157 + L-arginine; BPC 157 + L-NAME + L-arginine), except the defect did not change. Thereby at day 1 and 7, saline, L-NAME, L-arginine and L-NAME + L-arginine failed (defect was still open and large adhesions present). -----

CONCLUSION:

The therapeutic effect was achieved with BPC 157 alone or in combination with L-NAME and L-arginine as it was able to consolidate the stimulating and inhibiting effects of the NO-system towards more effective healing recruiting vessels.

• Keywords (english): Animals; Anti-Ulcer Agents/pharmacology; Anti-Ulcer Agents/therapeutic use; Arginine/pharmacology; Arginine/therapeutic use; Cecum/blood supply; Cecum/drug effects; Cecum/injuries; Disease Models, Animal; Drug Therapy, Combination/methods; Humans; Intestinal Perforation/drug therapy; Male; NG-Nitroarginine Methyl Ester/pharmacology; NG-Nitroarginine Methyl Ester/therapeutic use; Nitric Oxide/metabolism; Peptide Fragments/pharmacology; Peptide Fragments/therapeutic use; Proteins/pharmacology; Proteins/therapeutic use; Rats; Rats, Wistar; Treatment Outcome; Wound Healing/drug effects
• Language: english
• Publication type: Scientific paper - Original scientific paper
• Publication status: Published
• Peer review: Peer review
• Publication version: Published version
• Journal title: World Journal of Gastroenterology
• Numbering: vol. 24, no. 48, pp. 5462-5476
• p-ISSN: 1007-9327
• DOI: https://doi.org/10.3748/wjg.v24.i48.5462
• URN:NBN: urn:nbn:hr:105:486461
• Publication: 2018-12-28
• Document URL: https://doi.org/10.3748/wjg.v24.i48.5462
• Type of resource: Text
• Access conditions: Open access
• Created on: 2024-03-28 09:58:50