prikaz prve stranice dokumenta Bypassing major venous occlusion and duodenal lesions in rats, and therapy with the stable gastric pentadecapeptide BPC 157, L-NAME and L-arginine
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Scientific paper - Original scientific paper
Bypassing major venous occlusion and duodenal lesions in rats, and therapy with the stable gastric pentadecapeptide BPC 157, L-NAME and L-arginine
World Journal of Gastroenterology, 24 (2018), 47; 5366-5378. https://doi.org/10.3748/wjg.v24.i47.5366
Amić, Fedor; Drmić, Domagoj; Bilić, Zdenko; Krežić, Ivan; Žizek, Helena; Peklić, Marina; Kliček, Robert; Pajtak, Alen; Amić, Enio; Vidović, Tinka; Rakić, Mislav; Milković Periša, Marija; Horvat Pavlov, Katarina; Kokot, Antonio; Tvrdeić, Ante; Boban Blagaić, Alenka; Zovak, Mario; Seiwerth, Sven; Sikirić, Predrag More authors...

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Amić, F., Drmić, D., Bilić, Z., Krežić, I., Žizek, H., Peklić, M. ... Sikirić, P. (2018). Bypassing major venous occlusion and duodenal lesions in rats, and therapy with the stable gastric pentadecapeptide BPC 157, L-NAME and L-arginine. World Journal of Gastroenterology, 24. (47), 5366-5378. doi: 10.3748/wjg.v24.i47.5366

Amić, Fedor, et al. "Bypassing major venous occlusion and duodenal lesions in rats, and therapy with the stable gastric pentadecapeptide BPC 157, L-NAME and L-arginine." World Journal of Gastroenterology, vol. 24, no. 47, 2018, pp. 5366-5378. https://doi.org/10.3748/wjg.v24.i47.5366

Amić, Fedor, Domagoj Drmić, Zdenko Bilić, Ivan Krežić, Helena Žizek, Marina Peklić, Robert Kliček, et al. "Bypassing major venous occlusion and duodenal lesions in rats, and therapy with the stable gastric pentadecapeptide BPC 157, L-NAME and L-arginine." World Journal of Gastroenterology 24, no. 47 (2018): 5366-5378. https://doi.org/10.3748/wjg.v24.i47.5366

Amić, F., et al. (2018) 'Bypassing major venous occlusion and duodenal lesions in rats, and therapy with the stable gastric pentadecapeptide BPC 157, L-NAME and L-arginine', World Journal of Gastroenterology, 24(47), pp. 5366-5378. doi: 10.3748/wjg.v24.i47.5366

Amić F, Drmić D, Bilić Z, Krežić I, Žizek H, Peklić M, and sur.. Bypassing major venous occlusion and duodenal lesions in rats, and therapy with the stable gastric pentadecapeptide BPC 157, L-NAME and L-arginine. World Journal of Gastroenterology [Internet]. 2018 December 21 [cited 2024 April 16];24(47):5366-5378. doi: 10.3748/wjg.v24.i47.5366

F. Amić, et al., "Bypassing major venous occlusion and duodenal lesions in rats, and therapy with the stable gastric pentadecapeptide BPC 157, L-NAME and L-arginine", World Journal of Gastroenterology, vol. 24, no. 47, pp. 5366-5378, December 2018. [Online]. Available at: https://urn.nsk.hr/urn:nbn:hr:105:481354. [Accessed: 16 April 2024]

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Title (english)Bypassing major venous occlusion and duodenal lesions in rats, and therapy with the stable gastric pentadecapeptide BPC 157, L-NAME and L-arginine
AuthorFedor Amić
AuthorDomagoj Drmić
AuthorZdenko Bilić
AuthorIvan Krežić
AuthorHelena Žizek
AuthorMarina Peklić
AuthorRobert Kliček
AuthorAlen Pajtak
AuthorEnio Amić
AuthorTinka Vidović
AuthorMislav Rakić
AuthorMarija Milković Periša
AuthorKatarina Horvat Pavlov
AuthorAntonio Kokot
AuthorAnte Tvrdeić
AuthorAlenka Boban Blagaić
AuthorMario Zovak
AuthorSven Seiwerth
AuthorPredrag Sikirić
Author's institutionUniversity of Zagreb
School of Medicine
Scientific / art field,
discipline and subdiscipline		BIOMEDICINE AND HEALTHCARE
Clinical Medical Sciences
Abstract (english)
AIM: To investigate whether duodenal lesions induced by major venous occlusions can be attenuated by BPC 157 regardless nitric oxide (NO) system involvement. ----- METHODS: Male Wistar rats underwent superior anterior pancreaticoduodenal vein (SAPDV)-ligation and were treated with a bath at the ligated SAPDV site (BPC 157 10 μg, 10 ng/kg per 1 mL bath/rat; L-NAME 5 mg/kg per 1 mL bath/rat; L-arginine 100 mg/kg per 1 mL bath/rat, alone and/or together; or BPC 157 10 μg/kg instilled into the rat stomach, at 1 min ligation-time). We recorded the vessel presentation (filled/appearance or emptied/disappearance) between the 5 arcade vessels arising from the SAPDV on the ventral duodenum side, the inferior anterior pancreaticoduodenal vein (IAPDV) and superior mesenteric vein (SMV) as bypassing vascular pathway to document the duodenal lesions presentation; increased NO- and oxidative stress [malondialdehyde (MDA)]-levels in duodenum. ----- RESULTS: Unlike the severe course in the SAPDV-ligated controls, after BPC 157 application, the rats exhibited strong attenuation of the mucosal lesions and serosal congestion, improved vessel presentation, increased interconnections, increased branching by more than 60% from the initial value, the IAPDV and SMV were not congested. Interestingly, after 5 min and 30 min of L-NAME and L-arginine treatment alone, decreased mucosal and serosal duodenal lesions were observed; their effect was worsened at 24 h, and no effect on the collateral vessels and branching was seen. Together, L-NAME+L-arginine antagonized each other's response, and thus, there was an NO-related effect. With BPC 157, all SAPDV-ligated rats receiving L-NAME and/or L-arginine appeared similar to the rats treated with BPC 157 alone. Also, BPC 157 in SAPDV-ligated rats normalized levels of NO and MDA, two oxidative stress markers, in duodenal tissues. ----- CONCLUSION: BPC 157, rapidly bypassing occlusion, rescued the original duodenal flow through IAPDV to SMV flow, an effect related to the NO system and reduction of free radical formation.
Keywords (english)
Languageenglish
Publication typeScientific paper - Original scientific paper
Publication statusPublished
Peer reviewPeer review
Publication versionPublished version
Journal titleWorld Journal of Gastroenterology
Numberingvol. 24, no. 47, pp. 5366-5378
p-ISSN1007-9327
DOIhttps://doi.org/10.3748/wjg.v24.i47.5366
URN:NBNurn:nbn:hr:105:481354
Publication2018-12-21
Document URLhttps://doi.org/10.3748/wjg.v24.i47.5366
Type of resourceText
Access conditionsOpen access
Terms of useLicence In copyright icon
Created on2024-03-28 10:00:28