prikaz prve stranice dokumenta Recombinant human bone morphogenetic protein 6 delivered within autologous blood coagulum restores critical size segmental defects of ulna in rabbits
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Scientific paper - Original scientific paper
Recombinant human bone morphogenetic protein 6 delivered within autologous blood coagulum restores critical size segmental defects of ulna in rabbits
JBMR Plus, 3 (2018), 5; e10085. https://doi.org/10.1002/jbm4.10085
Grgurević, Lovorka; Oppermann, Hermann; Pećin, Marko; Erjavec, Igor; Capak, Hrvoje; Pauk, Martina; Karlović, Sven; Kufner, Vera; Lipar, Marija; Bubić Špoljar, Jadranka; Bordukalo-Nikšić, Tatjana; Matičić, Dražen; Perić, Mihaela; Windhager, Reinhard; Sampath, T Kuber; Vukičević, Slobodan More authors...

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Grgurević, L., Oppermann, H., Pećin, M., Erjavec, I., Capak, H., Pauk, M. ... Vukičević, S. (2018). Recombinant human bone morphogenetic protein 6 delivered within autologous blood coagulum restores critical size segmental defects of ulna in rabbits. JBMR Plus, 3. (5). doi: 10.1002/jbm4.10085

Grgurević, Lovorka, et al. "Recombinant human bone morphogenetic protein 6 delivered within autologous blood coagulum restores critical size segmental defects of ulna in rabbits." JBMR Plus, vol. 3, no. 5, 2018. https://doi.org/10.1002/jbm4.10085

Grgurević, Lovorka, Hermann Oppermann, Marko Pećin, Igor Erjavec, Hrvoje Capak, Martina Pauk, Sven Karlović, et al. "Recombinant human bone morphogenetic protein 6 delivered within autologous blood coagulum restores critical size segmental defects of ulna in rabbits." JBMR Plus 3, no. 5 (2018). https://doi.org/10.1002/jbm4.10085

Grgurević, L., et al. (2018) 'Recombinant human bone morphogenetic protein 6 delivered within autologous blood coagulum restores critical size segmental defects of ulna in rabbits', JBMR Plus, 3(5). doi: 10.1002/jbm4.10085

Grgurević L, Oppermann H, Pećin M, Erjavec I, Capak H, Pauk M, and sur.. Recombinant human bone morphogenetic protein 6 delivered within autologous blood coagulum restores critical size segmental defects of ulna in rabbits. JBMR Plus [Internet]. 2018 [cited 2024 December 29];3(5). doi: 10.1002/jbm4.10085

L. Grgurević, et al., "Recombinant human bone morphogenetic protein 6 delivered within autologous blood coagulum restores critical size segmental defects of ulna in rabbits", JBMR Plus, vol. 3, no. 5, 2018. [Online]. Available at: https://urn.nsk.hr/urn:nbn:hr:105:915117. [Accessed: 29 December 2024]

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Metadata
Title (english)Recombinant human bone morphogenetic protein 6 delivered within autologous blood coagulum restores critical size segmental defects of ulna in rabbits
AuthorLovorka Grgurević
AuthorHermann Oppermann
AuthorMarko Pećin
AuthorIgor Erjavec
AuthorHrvoje Capak
AuthorMartina Pauk
AuthorSven Karlović
AuthorVera Kufner
AuthorMarija Lipar
AuthorJadranka Bubić Špoljar
AuthorTatjana Bordukalo-Nikšić
AuthorDražen Matičić
AuthorMihaela Perić
AuthorReinhard Windhager
AuthorT Kuber Sampath
AuthorSlobodan Vukičević
Author's institutionUniversity of Zagreb
School of Medicine
Scientific / art field,
discipline and subdiscipline		BIOMEDICINE AND HEALTHCARE
Clinical Medical Sciences
Abstract (english)
BMP2 and BMP7, which use bovine Achilles tendon-derived absorbable collagen sponge and bovine bone collagen as scaffold, respectively, have been approved as bone graft substitutes for orthopedic and dental indications. Here, we describe an osteoinductive autologous bone graft substitute (ABGS) that contains recombinant human BMP6 (rhBMP6) dispersed within autologous blood coagulum (ABC) scaffold. The ABGS is created as an injectable or implantable coagulum gel with rhBMP6 binding tightly to plasma proteins within fibrin meshwork, as examined by dot-blot assays, and is released slowly as an intact protein over 6 to 8 days, as assessed by ELISA. The biological activity of ABGS was examined in vivo in rats (Rattus norvegicus) and rabbits (Oryctolagus cuniculus). In a rat subcutaneous implant assay, ABGS induced endochondral bone formation, as observed by histology and micro-CT analyses. In the rabbit ulna segmental defect model, a reproducible and robust bone formation with complete bridging and restoration of the defect was observed, which is dose dependent, as determined by radiographs, micro-CT, and histological analyses. In ABGS, ABC scaffold provides a permissive environment for bone induction and contributes to the use of lower doses of rhBMP6 compared with BMP7 in bovine bone collagen as scaffold. The newly formed bone undergoes remodeling and establishes cortices uniformly that is restricted to implant site by bridging with host bone. In summary, ABC carrier containing rhBMP6 may serve as an osteoinductive autologous bone graft substitute for several orthopedic applications that include delayed and nonunion fractures, anterior and posterior lumbar interbody fusion, trauma, and nonunions associated with neurofibromatosis type I.
Languageenglish
Publication typeScientific paper - Original scientific paper
Publication statusPublished
Peer reviewPeer review
Publication versionPublished version
Journal titleJBMR Plus
Numberingvol. 3, no. 5, e10085
p-ISSN2473-4039
DOIhttps://doi.org/10.1002/jbm4.10085
URN:NBNurn:nbn:hr:105:915117
Publication2018
Document URLhttps://doi.org/10.1002/jbm4.10085
Type of resourceText
Access conditionsOpen access
Terms of useLicence In copyright icon
Created on2024-03-28 10:21:21