Aberrant glycosylation of Igg heavy chain in multiple myeloma

Scientific paper - Original scientific paper

• Author: Igor Aurer
• Author: Gordan Lauc
• Author: Jerka Dumić
• Author: Dubravko Rendić
• Author: Danica Matišić
• Author: Marija Miloš
• Author: Marija Heffer-Lauc
• Author: Mirna Flogel
• Author: Boris Labar
• Author's institution: University of Zagreb; School of Medicine
• Scientific / art field, discipline and subdiscipline: BIOMEDICINE AND HEALTHCARE; Clinical Medical Sciences

Abstract (english)

Although the majority of eukaryotic proteins are glycosylated, there is a dearth of knowledge regarding protein sugar moieties and their changes in disease. Most multiple myeloma cases are characterized by production of monoclonal immunoglobulins (Ig). We studied galactosylation and sialylation of IgG heavy chains in 16 patients with IgG myeloma using lectin blotting and densitometry. In comparison to age and sex matched controls, galactosylation was reduced in multiple myeloma (median 317 vs. 362, range 153-410 vs. 309-447 relative units, p = 0.015, Student's t-test). Sialylation was stage dependent; samples from patients with stage IIA had lowest amounts of sialic acid, IIIA intermediate and IIIB highest (142.6 vs. 185.9 vs. 248.5 relative units, correlation coefficient r = 0.55). Both galactosylation and sialylation levels were independent of age, sex, treatment type, response to treatment, disease duration and IgG and b2 microglobulin concentration. These data indicate that multiple myeloma is characterized by aberrant immunoglobulin glycosylation.

Abstract (croatian)

Iako je većina eukariotskih proteina glikozilirana, nema puno podataka o šećerima vezanima na proteine niti o njihovim promjenama u pojedinim bolestima. Multipli mijelom je većinom karakteriziran stvaranjem monoklonskih imunoglobulina (Ig). Istraživali smo galaktozilaciju i sijalilaciju IgG teškog lanca u 16 bolesnika s IgG mijelomom koristeći specifično vezivanje lektina i denzitometriju. U usporedbi s kontrolama podjednake dobi i spola je u multiplom mijelomu smanjena galaktozilacija (medijan 317 naprema 362, raspon 153–410 naprema 309–447 relativnih jedinica, p=0.015 koristeći Studentov t-test). Sijalilacija je ovisila o stadiju. Najmanje sijalinske kiseline je nađeno u uzorcima bolesnika sa stadijem IIA, nešto više u onih s IIIA, a najviše u IIIB stadiju (142,6 naprema 185,9 naprema 248,5 relativnih jedinica, koeficijent korelacije r=0,55). Ni razina galaktozilacije niti sijalilacije nisu ovisile o dobi, spolu, vrsti liječenja, odgovoru na liječenje, trajanju bolesti, koncentraciji IgG ili β2 mikroglobulina. Ovi podaci ukazuju da je multipli mijelom karakteriziran poremećajem glikozilacije imunoglobulina.

• Keywords (english): Immunoglobulin G - metabolism; Immunoglobulin Heavy Chains - metabolism; Multiple Myeloma - immunology; Adult; Aged; Female; Galactose - metabolism; Glycosylation; Humans; Male; Middle Aged; N-Acetylneuraminic Acid - metabolism
• Language: english
• Publication type: Scientific paper - Original scientific paper
• Publication status: Published
• Peer review: Peer review
• Publication version: Published version
• Journal title: Collegium Antropologicum
• Numbering: vol. 31, no. 1, pp. 247-251
• p-ISSN: 0350-6134
• URN:NBN: urn:nbn:hr:105:851262
• Publication: 2007-03
• Document URL: https://hrcak.srce.hr/27277
• Type of resource: Text
• Access conditions: Open access
• Created on: 2024-03-28 10:56:59