prikaz prve stranice dokumenta Morphometric and DNA image analysis of bronchoalveolar lavage fluid macrophages nuclei in interstitial lung diseases with lymphocytic alveolitis
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Scientific paper - Original scientific paper
Morphometric and DNA image analysis of bronchoalveolar lavage fluid macrophages nuclei in interstitial lung diseases with lymphocytic alveolitis
Collegium Antropologicum, 34 (2010), 1; 123-129. urn:nbn:hr:105:917915
Smojver-Ježek, Silvana; Peroš-Golubičić, Tatjana; Tekavec-Trkanjec, Jasna; Alilović, Marija; Vrabec-Branica, Božica; Juroš, Zrinka; Mažuranić, Ivica

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Smojver Ježek, S., Peroš Golubičić, T., Tekavec Trkanjec, J., Alilović, M., Vrabec Branica, B., Juroš, Z. & Mažuranić, I. (2010). Morphometric and DNA image analysis of bronchoalveolar lavage fluid macrophages nuclei in interstitial lung diseases with lymphocytic alveolitis . Collegium Antropologicum, 34. (1), 123-129. Retrieved from https://urn.nsk.hr/urn:nbn:hr:105:917915

Smojver Ježek, Silvana, et al. "Morphometric and DNA image analysis of bronchoalveolar lavage fluid macrophages nuclei in interstitial lung diseases with lymphocytic alveolitis ." Collegium Antropologicum, vol. 34, no. 1, 2010, pp. 123-129. https://urn.nsk.hr/urn:nbn:hr:105:917915

Smojver Ježek, Silvana, Tatjana Peroš Golubičić, Jasna Tekavec Trkanjec, Marija Alilović, Božica Vrabec Branica, Zrinka Juroš and Ivica Mažuranić. "Morphometric and DNA image analysis of bronchoalveolar lavage fluid macrophages nuclei in interstitial lung diseases with lymphocytic alveolitis ." Collegium Antropologicum 34, no. 1 (2010): 123-129. https://urn.nsk.hr/urn:nbn:hr:105:917915

Smojver Ježek, S., et al. (2010) 'Morphometric and DNA image analysis of bronchoalveolar lavage fluid macrophages nuclei in interstitial lung diseases with lymphocytic alveolitis ', Collegium Antropologicum, 34(1), pp. 123-129. Available at: https://urn.nsk.hr/urn:nbn:hr:105:917915 (Accessed 28 April 2024)

Smojver Ježek S, Peroš Golubičić T, Tekavec Trkanjec J, Alilović M, Vrabec Branica B, Juroš Z, and sur.. Morphometric and DNA image analysis of bronchoalveolar lavage fluid macrophages nuclei in interstitial lung diseases with lymphocytic alveolitis . Collegium Antropologicum [Internet]. 2010 March [cited 2024 April 28];34(1):123-129. Available at: https://urn.nsk.hr/urn:nbn:hr:105:917915

S. Smojver Ježek, et al., "Morphometric and DNA image analysis of bronchoalveolar lavage fluid macrophages nuclei in interstitial lung diseases with lymphocytic alveolitis ", Collegium Antropologicum, vol. 34, no. 1, pp. 123-129, March 2010. [Online]. Available at: https://urn.nsk.hr/urn:nbn:hr:105:917915. [Accessed: 28 April 2024]

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Title (english)Morphometric and DNA image analysis of bronchoalveolar lavage fluid macrophages nuclei in interstitial lung diseases with lymphocytic alveolitis
Title (croatian)Morfometrijska i DNA image analiza jezgara makrofaga u bronhoalveolarnom lavatu bolesnika sa bolestima plućnog intersticija i limfocitnim alveolitisom
AuthorSilvana Smojver-Ježek
AuthorTatjana Peroš-Golubičić
AuthorJasna Tekavec-Trkanjec
AuthorMarija Alilović
AuthorBožica Vrabec-Branica
AuthorZrinka Juroš
AuthorIvica Mažuranić
Author's institutionUniversity of Zagreb
School of Medicine
Scientific / art field,
discipline and subdiscipline		BIOMEDICINE AND HEALTHCARE
Clinical Medical Sciences
Abstract (english)
Lymphocytic alveolitis is a characteristic of diverse interstitial lung diseases (ILD-s), but macrophages are often more numerous cell population in bronchoalveolar lavage fluid (BALF). Aim of this study is to analyze morphometric characteristics of macrophages nuclei in BALF in patients with ILD-s and to detect possible differences allowing distinguishing sarcoidosis from other lymphocytic alveolitis ILD-s. Thirty-one patient with interstitial lung disease who had lymphocytic alveolitis in BALF cell count (17 sarcoidosis and 14 other ILD-s) and nine controls were included in the study. The following patients data were numbered: age, lymphocyte percentage and CD4/CD8 ratio in BALF. Investigated morphometric parameters of macrophages nuclei were: area, outline, maximal radius, minimal radius, length, breadth, form factor (FF), elongation factor (EF) and DNA image cytometry ploidy status determined with Van Velthoven method. Predicted classifications in classification matrix (forward step-wise method in multivariate discriminant function analysis) based on macrophages nuclei length mean, minimum and maximum, breadth SD, FF mean and lymphocyte % were 100% (9/9) correct for control group, 88.235% (15/17) correct for sarcoidosis, and 92.857% (13/14) correct for other lymphocytic alveolitis ILD group. In total, 92.5% (37/40) of the examinees were correctly classified in particular group upon the observed variables.
Abstract (croatian)
Makrofagi su u većine bolesti plućnog intersticija, kao i u limfocitnom alveolitisu, najbrojnija stanična populacija u bronhoalveolarnom lavatu. Cilj rada bio je uz morfometrijsku analizu jezgara makrofaga u bronhoalveolarnom lavatu bolesnika sa bolestima plućnog intersticija procijeniti mogućnosti razlikovanja sarkoidoze od drugih bolesti intersticija koje se prezentiraju limfocitnim alveolitisom. U ispitivanje je uključen trideset i jedan bolesnik sa bolestima plućnog intersticija i limfocitnim alveolitisom u bronhoalveolarnim lavatu (17 bolesnika sa sarkoidozom i 14 bolesnika sa drugim bolestima) i 9 kontrolnih ispitanika. Bilježeni su dob bolesnika, postotak limfocita i omjer CD4 i CD8 T limfocita u bronhoalveolarnom lavatu. Analizirani su morfometrijski parametari jezgara makrofaga: površina, opseg, najveći polumjer, najmanji polumjer, duljina, širina, faktor oblika (FF), faktor izduženosti (EF), te DNA indeks određen metodom po Van Velthovenu. Multivariatnom diskriminativnom analizom uz korištenje stupnjevitog eliminiranja identificirane su varijable: prosjek, minimalne i maksimalne vijednosti duljine, SD širine, prosjek faktora oblika (FF) i prosjek postotka limfocita. Ove varijble omogućuju razlikovanje pojedinih skupina: potpuno razlikovanje (9/9) kontrolne skupine, 88,235% (15/17) razlikovanje skupine bolesnika sa sarkoidozom i 92,857% (13/14) skupine sa drugim bolestima plućnog intersticija i limfocitnim alveolitisom. Identificirane varijable omogućuju točno klasificiranje 92,5% (37/40) ispitanika.
Keywords (english)
Languageenglish
Publication typeScientific paper - Original scientific paper
Publication statusPublished
Peer reviewPeer review
Publication versionPublished version
Journal titleCollegium Antropologicum
Numberingvol. 34, no. 1, pp. 123-129
p-ISSN0350-6134
URN:NBNurn:nbn:hr:105:917915
Publication2010-03
Document URLhttps://hrcak.srce.hr/51252
Type of resourceText
Access conditionsOpen access
Terms of useLicence In copyright icon
Created on2024-03-28 11:42:06