Regionalne razlike u izraženosti molekularnih biljega tijekom formiranja proširene subplate zone u fetalnoj moždanoj kori čovjeka

doctoral thesis

• Title (english): Regional differences in expression of molecular markers during formation of the expanded subplate zone in the human fetal cerebral cortex
• Author: Alisa Junaković Munjas
• Mentor: Željka Krsnik (mentor)
• Committee member: Mario Vukšić (predsjednik povjerenstva)
• Committee member: Goran Sedmak (član povjerenstva)
• Committee member: Ivica Kostović (član povjerenstva)
• Granter: University of Zagreb; School of Medicine; Zagreb
• Defense date and country: 2024-03-25, Croatia
• Scientific / art field, discipline and subdiscipline: BIOMEDICINE AND HEALTHCARE; Basic Medical Sciences; Neuroscience
• Universal decimal classification (UDC): 61 - Medical sciences

Abstract

U doktorskoj disertaciji prikazane su regionalne citoarhitektonske razlike između čeone, tjemene, zatiljne i cingularne moždane kore u fazi ekspanzije i stvaranja subplate zone (od 13. do 15. TNZ). Pomoću imunohistokemijskih metoda na prenatalnom postmortalnom tkivu mozga čovjeka praćena je dinamika ekspresije molekularnih biljega. Rezultati su pokazali kako regionalne razlike između pojedinih izokortikalnih regija fetalne moždane kore čovjeka postaju vidljive rano tijekom prenatalnog razvoja, a postaju najistaknutije upravo tijekom razdoblja formiranja SP-a. Proces stvaranja SP-a iz dubokog dijela KP prikazan je biljegom SP neurona TBR1. Pokazano je i kako je obrazac formiranja SP-a važan kriterij za diferenciranje dorzalne izokortikalne i ventralne mezokortikalne cingularne moždane kore u ranom fetalnom razdoblju. Nadalje, karakteristike rane diferencijacije mezokortikalne cingularne moždane kore su: proširenje MZ, suženje KP i SVZ. Jedna od glavnih karakteristika mezokortikalnog dijela cingularne moždane kore je nepotpuna ekspanzija SP-a, a navedeno je prikazano pomoću biljega projekcijskih neurona. Analiza ranog razvoja cingularne moždane kore važna je radi njezine uloge u stvaranju ranih neuralnih krugova uključenih u ponašanje i emocije. Nadalje,
poznavanje regionalnog razvoja bitno je za razumijevanje arealne diferencijacije i kasnije funkcionalne specifikacije moždane kore što je preduvjet za razumijevanje neurorazvojnih poremećaja.

Abstract (english)

In the doctoral thesis, regional cytoarchitectonic differences between the frontal, parietal, occipital, and cingulate cortex were analyzed in the the subplate formation phase (13 to 15 PCW). Immunohistochemical methods were used on prenatal postmortem human brain tissue to analyze the molecular markers` expression pattern dynamics. The results showed that
regional differences between isocortical regions of the human fetal cortex become visible early during prenatal development, and are most prominent during the SP formation period. The SP formation process is shown with the SP neuron marker TBR1. Additionally, we showed that the SP formation pattern is an important criterion for differentiating the dorsal isocortical and ventral mesocortical cingulate cortex in the early fetal period. Furthermore, the early mesocortical cingulate cortex is characterized by the widening of the MZ and the narrowing of the CP and SVZ. One of the main characteristics of the mesocortical part of the cingulate cortex is an incomplete SP expansion, and this is shown in the results using projection neuron markers. The early cingulate cortex development analysis is important because of its involvement in the formation of early neural circuits involved in behavior and emotions. Furthermore, analysis of regional differences is essential for understanding areal differentiation and later functional specification of the cerebral cortex, which is a prerequisite for understanding diverse neurodevelopmental disorders.

• Keywords: Gyrus Cinguli; Moždana kora; Neuroni; Neurorazvojni poremećaji; Emocije
• Keywords (english): Gyrus Cinguli; Cerebral Cortex; Neurons; Neurodevelopmental Disorders; Emotions
• Language: croatian
• URN:NBN: urn:nbn:hr:105:557642
• Project: Number: PZS-2019-02-4710; Title: Uloga RNA vezujućeg proteina CELF 1 u neuronalnom razvoju čovjeka; Title: Role of RNA binding protein CELF1 in normal and abnormal human neuronal development; Acronym: CELF1NeuDev; Leader: Željka Krsnik; Jurisdiction: Croatia; Funder: HRZZ; Funding stream: Nema
• Project: Number: DOK-2020-01-5029; Title: Projekt razvoja karijera mladih istraživača – izobrazba novih doktora znanosti; Leader: Željka Krsnik; Jurisdiction: Croatia; Funder: HRZZ; Funding stream: DOK
• Project: Number: 8995; Title: Misterij egzosomskih glasnika: od uzbune u stanici do poruke drugim stanicama; Title: The mystery of exosomal messengers: from an alarm in the cell to a message to other cells; Leader: Željka Krsnik; Jurisdiction: Croatia; Funder: Zaklada Adris; Funding stream: donacija
• Project: Number: KK.01.1.1.01.0007; Title: Eksperimentalna i klinička istraživanja hipoksijsko-ishemijskog oštećenja mozga u perinatalnoj i odrasloj dobi; Leader: Miloš Judaš; Jurisdiction: eu; Funder: EK; Funding stream: EU
• Study programme: Title: Neuroscience; Study programme type: university; Study level: postgraduate; Academic / professional title: doktor/doktorica znanosti, područje biomedicine i zdravstvo (doktor/doktorica znanosti, područje biomedicine i zdravstvo)
• Type of resource: Text
• Extent: 120 str.
• File origin: Born digital
• Access conditions: Embargoed access; Embargo expiration date: 2025-03-26
• Created on: 2024-03-29 11:08:17