Praćenje utjecaja valproata na molekularni sastav medija tijekom kulture štakorskog zametka metodom infracrvene spektrometrije

master's thesis

• Title (english): Monitoring of valproate effect on the molecular composition of the medium during rat embryo culture assessed by infrared spectroscopy
• Author: Mario Rašić
• Mentor: Maja Balarin (mentor)
• Committee member: Ozren Gamulin (predsjednik povjerenstva)
• Committee member: Sanja Dolanski-Babić (član povjerenstva)
• Committee member: Maja Balarin (član povjerenstva)
• Granter: University of Zagreb; School of Medicine; (Department of Physics and Biophysics); Zagreb
• Defense date and country: 2016-07-15, Croatia
• Scientific / art field, discipline and subdiscipline: BIOMEDICINE AND HEALTHCARE; Basic Medical Sciences; Medical Biochemistry

Abstract

Uzorci medija u kojima su rasli štakorski zameci analizirani su FTIR spektroskopijom. Dobiveni spektri su uprosječeni, obrađeni pomoću Studentovog t-testa i multivarijatnom statističkom metodom analize principalne komponente (PCA). Pokazano je da se FTIR spektroskopijom može proučavati biološki materijal odnosno medij u kojem je rastao i razvijao se štakorski zametak. Također je ustvrđeno da se pomoću FTIR spektroskopije može pratiti utjecaj teratogenih supstanci poput valproata koji svojim učinkom mijenja embrij, a samim time i molekularni sastav medija u kojem je zametak rastao. Taj molekularni sastav je moguće pratiti kroz dane razvoja štakorskog zametka. Analiza uprosječenih spektara pokazala je da je utjecaj valproata najveći tijekom neurulacije štakorskog zametka, odnosno da je molekularni sastav medija kontrolne skupine i skupine s valproatom tada najrazličitiji. Pored toga, prikazano je kako je utjecaj valproata na molekularni sastav medija najmanji tijekom četrnaestog dana kulture štakorskog zametka, kada je on na samom kraju svog razvitka prije nego što postane fetus. Rezultati ovog diplomskog ukazuju da je FTIR spektroskopija izuzetno oruđe u proučavanju i praćenu utjecaja valproata na molekularni sastava medija tijekom kulture štakorskog zametka.

Abstract (english)

Samples of the rat embryo media in which they grew were analyzed by FTIR spectroscopy. The resulting spectra were averaged and analyzed using Student's t-test and multivariate statistical method, principal component analysis (PCA). It is shown that FTIR spectroscopy can be used to study the biological material such as the medium during rat embryo culture. It is also detected that by using FTIR spectroscopy we can monitor the impact of teratogenic substances such as valproic acid on rat embryos, which by its effect changes the embryo, and therefore the molecular composition of the medium during rat embryo culture. The molecular composition of medium can be followed through the days of the development of rat embryos. The analyzis of averaged spectra revealed that the valproic acid has the highest impact during neurulation of the rat embryo. At that period the molecular composition of the medium in control group and the group treated with valproate is the most different. In addition, it is shown how the impact of the valproate on the molecular composition of the medium is the least significant during the fourteenth day of rat embryo culture when it is in the final stage of its development, before it becomes a fetus. The results of this diploma thesis show that FTIR spectroscopy is an exceptional tool for observing and following the impact of valproate on the molecular composition of the medium during rat embryo culture.

• Keywords: valproat; FTIR; štakorski zametak; medij
• Keywords (english): valproate; FTIR; rat embryo; medium
• Language: croatian
• URN:NBN: urn:nbn:hr:105:749728
• Study programme: Title: Medicine; Study programme type: university; Study level: integrated undergraduate and graduate; Academic / professional title: doktor/doktorica medicine (doktor/doktorica medicine)
• Type of resource: Text
• File origin: Born digital
• Access conditions: Open access
• Created on: 2017-07-11 12:09:48