Zoledronate Bound to Ceramics Increases Ectopic Bone Volume Induced by rhBMP6 Delivered in Autologous Blood Coagulum in Rats

Scientific paper - Original scientific paper

• Author: Nikola Štoković
• Author: Natalia Ivanjko
• Author: Igor Erjavec
• Author: Anita Breski
• Author: Mihaela Perć
• Author: Slobodan Vukičević
• Author's institution: University of Zagreb; School of Medicine; (Department of Anatomy and Clinical Anatomy)
• Scientific / art field, discipline and subdiscipline: BIOMEDICINE AND HEALTHCARE; Basic Medical Sciences; Anatomy

Abstract (english)

Autologous bone graft substitute (ABGS) containing rhBMP6 in autologous blood coagulum (ABC) with synthetic ceramics is a novel therapeutic solution for bone repair. The aim of this study was to investigate whether the application of Zoledronate (ZOL) with ABGS might enhance the properties of newly formed bone. The effect of ZOL on bone induction was tested in a rat subcutaneous implant model. ZOL bound to synthetic ceramics was added into ABGS implants, and the quantity, quality, and longevity of the induced bone were assessed by micro-CT, histomorphometry, and histology over a period of 365 days. Local use of ZOL in the ABGS implants with ceramics had no influence on the bone volume (BV) on day 14 but subsequently significantly increased BV on days 35, 50, 105, 140, and 365 compared to the control implants. Locally applied ZOL had a similar effect in all of the applied doses (2–20 µg), while its systemic use on stimulating the BV of newly induced bone by ABGS depended on the time of application. BV was increased when ZOL was applied systemically on day 14 but had no effect when applied on day 35. The administration of ZOL bound to ceramics in ABGS increased and maintained the BV over a period of one year, offering a novel bone tissue engineering strategy for treating bone defects and spinal fusions.

• Keywords (english): BMP; BMP carrier; bisphosphonates; bone morphogenetic proteins; bone regeneration; osteogrow; zoledronate
• Language: english
• Publication type: Scientific paper - Original scientific paper
• Publication status: Published
• Peer review: Peer review
• Publication version: Published version
• Journal title: Biomedicines
• Numbering: vol. 9, no. 10, 1487
• e-ISSN: 2227-9059
• DOI: https://doi.org/10.3390/biomedicines9101487
• URN:NBN: urn:nbn:hr:105:996950
• Publication: 2021-10-16
• Project: Number: KK.01.1.1.01.0008; Title (croatian): Znanstveni centar izvrsnosti za reproduktivnu i regenerativnu medicinu – istraživanja novih platformi i potencijala; Title (english): Reproductive, and Regenerative Medicine-Exploring New Platforms and Potentials; Acronym: CERRM; Leader: Slobodan Vukičević; Jurisdiction: eu
• Document URL: https://www.mdpi.com/2227-9059/9/10/1487
• Type of resource: Text
• Access conditions: Open access
• Created on: 2022-04-01 11:12:59