| Abstract | Ženke Wistar štakora, 200g, randomizirano podijeljene, retrobulbarno su tretirane sa: L-NAME (0,1ml/0,2mg/oko) i BPC 157 (0,1ml/0,2μg/oko, 20 minuta nakon primjene L-NAME); L-NAME (0,1ml/0,2mg/oko) i BPC 157 (0,1ml/0,2ng/oko, 20 minuta nakon primjene L-NAME); L-NAME (0,1ml/0,2mg/oko) i 0,9% NaCl (0,1ml/oko, 20 minuta nakon primjene L-NAME). Kontrolne su životinje istovremeno retrobulbarno primale jednaki volumen 0,9% NaCl.
USB mikroskopskom kamerom „Veho discovery VMS-004 deluxe“ i „VOLK“ digital wide field lupom za indirektnu oftalmolskopiju snimana je očna pozadina. Inicijalno, životinje su snimane: prije retrobulbarne aplikacije L-NAME (odnosno 0,9% NaCl); 20 minuta nakon aplikacije L-NAME (odnosno 0,9% NaCl); ponovno nakon aplikacije BPC 157 (odnosno 0,9% NaCl) te nakon 20 minuta. Nadalje snimane su 1. i 2. dan nakon aplikacije te nakon 1., 2. i 4. tjedna (neposredno prije žrtvovanja).
Očna je pozadina analizirana računalnim programom za analizu fotografija te patohistološkom analizom bulbusa na standardnim rezovima, uklopljenog u parafin i bojenog hemalaun eozinom.
Snimano ponašanje životinja uključivalo je vrijeme imobilnosti životinja nakon stavljanja na nepoznatu podlogu i reakciju životinja na podražaj plavo-zelenom rukavicom.
Retrobulbamo aplicirana L-NAME uzrokovala je vazokonstrikciju arterija mrežnice, ishemiju mrežnice te značajnu imobilnost životinja.
Retrobulbarna primjena BPC 157 dovela je do oporavka mrežnične ishemije te je spriječila njenu atrofiju. Ponašanje životinja bilo je normalno. |
| Abstract (english) | Female Wistar rats, 200 g, randomly assigned, were treated retrobulbary with: L-NAME (0.1ml/0.2mg/eye) and BPC 157 (0.1ml/0.2μg/eye 20 minutes after L-NAME application); L-NAME (0.1ml/0.2mg/eye) and BPC 157 (0.1ml/0.2ng/eye 20 minutes after L-NAME); L-NAME (0.1ml/0.2 mg/eye) and 0.9% NaCl (0.1ml/eye 20 minutes after L-NAME application).
Control animals simultaneously received retrobulbar equivolume of 0.9% NaCl.
With a USB microscope camera „Veho discovery VMS-004 deluxe“ and "VOLK" digital wide field loupe for indirect ophthalmoscope eye fundus was recorded in the following time periods: before retrobulbar application of L-NAME (or 0.9% NaCl); 20 minutes after L-NAME (or 0.9% NaCl); again after BPC 157 (or 0.9% NaCl), and 20 minutes after. Furthermore, recording was performed at the post-application day 1, 2, and 7, 14, 28 (last day, just before the sacrifice).
The fundus was analysed by photo analysis software and by histopathologic eyeball analysis on standard sections, paraffin embedded and hemalaun-eosin stained.
The animals behavior was filmed, observing the following parameters: time of immobility and reaction time to visual stimuli (blue-green glove).
The retrobulbar application of L-NAME causes the vasoconstriction of the retina's arteries, retinal ischemia, while retrobulbar application of BPC 157 leads to retinal ischemia recovery, prevents its atrophy and restores normal animal behavior. |
