prikaz prve stranice dokumenta Persistent accumulation of 4-hydroxynonenal-protein adducts in gastric mucosa after Helicobacter pylori eradication
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Scientific paper - Original scientific paper
Persistent accumulation of 4-hydroxynonenal-protein adducts in gastric mucosa after Helicobacter pylori eradication
Collegium Antropologicum, 33 (2009), 3; 815-821. urn:nbn:hr:105:305431
Cherkas, Andriy; Yelisyeyeva, Olha; Semen, Khrystyna; Žarković, Kamelija; Kaminskyy, Danylo; Čipak Gašparović, Ana; Jaganjac, Morana; Lutsyk, Alexander; Waeg, Georg; Žarković, Neven

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Cherkas, A., Yelisyeyeva, O., Semen, K., Žarković, K., Kaminskyy, D., Čipak Gašparović, A. ... Žarković, N. (2009). Persistent accumulation of 4-hydroxynonenal-protein adducts in gastric mucosa after Helicobacter pylori eradication . Collegium Antropologicum, 33. (3), 815-821. Retrieved from https://urn.nsk.hr/urn:nbn:hr:105:305431

Cherkas, Andriy, et al. "Persistent accumulation of 4-hydroxynonenal-protein adducts in gastric mucosa after Helicobacter pylori eradication ." Collegium Antropologicum, vol. 33, no. 3, 2009, pp. 815-821. https://urn.nsk.hr/urn:nbn:hr:105:305431

Cherkas, Andriy, Olha Yelisyeyeva, Khrystyna Semen, Kamelija Žarković, Danylo Kaminskyy, Ana Čipak Gašparović, Morana Jaganjac, Alexander Lutsyk, Georg Waeg and Neven Žarković. "Persistent accumulation of 4-hydroxynonenal-protein adducts in gastric mucosa after Helicobacter pylori eradication ." Collegium Antropologicum 33, no. 3 (2009): 815-821. https://urn.nsk.hr/urn:nbn:hr:105:305431

Cherkas, A., et al. (2009) 'Persistent accumulation of 4-hydroxynonenal-protein adducts in gastric mucosa after Helicobacter pylori eradication ', Collegium Antropologicum, 33(3), pp. 815-821. Available at: https://urn.nsk.hr/urn:nbn:hr:105:305431 (Accessed 17 November 2024)

Cherkas A, Yelisyeyeva O, Semen K, Žarković K, Kaminskyy D, Čipak Gašparović A, and sur.. Persistent accumulation of 4-hydroxynonenal-protein adducts in gastric mucosa after Helicobacter pylori eradication . Collegium Antropologicum [Internet]. 2009 September [cited 2024 November 17];33(3):815-821. Available at: https://urn.nsk.hr/urn:nbn:hr:105:305431

A. Cherkas, et al., "Persistent accumulation of 4-hydroxynonenal-protein adducts in gastric mucosa after Helicobacter pylori eradication ", Collegium Antropologicum, vol. 33, no. 3, pp. 815-821, September 2009. [Online]. Available at: https://urn.nsk.hr/urn:nbn:hr:105:305431. [Accessed: 17 November 2024]

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Metadata
Title (english)Persistent accumulation of 4-hydroxynonenal-protein adducts in gastric mucosa after Helicobacter pylori eradication
Title (croatian)Povećano nakupljanje 4-hidroksinonenal-proteinskih konjugata u želučanoj sluznici zaostaje nakon izlječenja Helicobacter pylori
AuthorAndriy Cherkas
AuthorOlha Yelisyeyeva
AuthorKhrystyna Semen
AuthorKamelija Žarković
AuthorDanylo Kaminskyy
AuthorAna Čipak Gašparović
AuthorMorana Jaganjac
AuthorAlexander Lutsyk
AuthorGeorg Waeg
AuthorNeven Žarković
Author's institutionUniversity of Zagreb
School of Medicine
Scientific / art field,
discipline and subdiscipline		BIOMEDICINE AND HEALTHCARE
Clinical Medical Sciences
Abstract (english)
Recent studies indicate that oxidative stress caused by Helicobacter pylori and insufficient host antioxidant defense could play important role in pathogenesis of gastrointestinal ulcerations. By specific monoclonal antibodies we have detected weak presence of the major lipid peroxidation bioactive marker 4-hydroxynonenal (HNE) in healthy human gastric mucosa, which strongly increased in case of H. pylori-associated peptic ulcer. Considering physiological presence of HNE on one hand, and high prevalence of H. pylori associated disorders on the other, evaluation of oxidative stress after treatment is important. Therefore, in current study immunohistochemical accumulation and distribution of HNE-protein adducts in gastric mucosa was evaluated with 21 patients having H. pylori-associated duodenal peptic ulcer (DPU) before and one month after eradication of H. pylori. Although dramatic decrease in histological manifestations of inflammation was demonstrated after eradication of H. pylori, initially high immunopositivity for the HNE-protein adducts remained elevated in antrum and even increased in stomach corpus. The observed accumulation and redistribution to higher grades of HNE-immunopositivity in nuclei of glandular cells in gastric corpus indicate augmentation of oxidative stress after treatment and open possibilities for adjuvant antioxidant treatments to protect gastric mucosa from progressive oxidative stress after eradication of H. pylori infection.
Abstract (croatian)
Dosadašnja istraživanja pokazala su da bi oksidacijski stres uzrokovan bakterijom Helicobacter pylori, zajedno sa smanjenom antioksidacijskom zaštitom domaćina, mogao igrati značajnu ulogu u patogenezi želučanog ulkusa. U prijašnjem radu dokazali smo izvornim monoklonskim protutijelima slabi pozitivitet 4-hidroksinonenala (HNE), glavnog bioaktivnog biljega lipidne peroksidacije, u fiziološkim uvjetima u zdravoj ljudskoj želučanoj sluznici. Pozitivitet HNEa pojačavao se u slučaju infekcije s H. pylori kao i kod peptičnog ulcua. Obzirom na fiziološku prisutnost HNEa s jedne strane i visoke učestalosti bolesti vezane uz H. pylori s druge strane, procjena oksidacijskog stresa nakon tretmana mogla bi biti važan progostički parametar. U ovom radu praćena je imunohistokemijska distribucija HNE-proteinskih konjugata u želučanoj sluznici kod 21 pacijenta sa ulcusom dvanaesnika (DPU) izazvanim H. pylori prije liječenja i mjesec dana poslije liječenja. Uočeno povećanje i preraspodjela HNE-pozitiviteta u jezgri žljezdanih stanica u tijelu želuca ukazuju na povećanje oksidacijskog stresa nakon tretmana te time pruža mogućnost adjuvantnog antioksidacijskog tretmana za zaštitu sluznice želuca od razvoja oksidacijskog stresa i lipidne peroksidacije u i nakon infekcije s H. pylori. Nakon izliječenja H. pylori, histološki pokazatelji upale su drastično sniženi, no količina HNE- proteinskih konjugata je i dalje povišena u antrumu, te dodatno povišena u tijelu želuca.
Keywords (english)
Languageenglish
Publication typeScientific paper - Original scientific paper
Publication statusPublished
Peer reviewPeer review
Publication versionPublished version
Journal titleCollegium Antropologicum
Numberingvol. 33, no. 3, pp. 815-821
p-ISSN0350-6134
URN:NBNurn:nbn:hr:105:305431
Publication2009-09
Document URLhttps://hrcak.srce.hr/41824
Type of resourceText
Access conditionsOpen access
Terms of useLicence In copyright icon
Created on2024-03-28 11:27:02