prikaz prve stranice dokumenta Detection of VAMP Proteolysis by Tetanus and Botulinum Neurotoxin Type B In Vivo with a Cleavage-Specific Antibody
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Scientific paper - Original scientific paper
Detection of VAMP Proteolysis by Tetanus and Botulinum Neurotoxin Type B In Vivo with a Cleavage-Specific Antibody
International Journal of Molecular Sciences, 23 (2022), 8; 4355. https://doi.org/10.3390/ijms23084355
Fabris, Federico; Šoštarić, Petra; Matak, Ivica; Binz, Thomas; Toffan, Anna; Simonato, Morena; Montecucco, Cesare; Pirazzini, Marco; Rossetto, Ornella

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Fabris, F., Šoštarić, P., Matak, I., Binz, T., Toffan, A., Simonato, M. ... Rossetto, O. (2022). Detection of VAMP Proteolysis by Tetanus and Botulinum Neurotoxin Type B In Vivo with a Cleavage-Specific Antibody. International Journal of Molecular Sciences, 23. (8). doi: 10.3390/ijms23084355

Fabris, Federico, et al. "Detection of VAMP Proteolysis by Tetanus and Botulinum Neurotoxin Type B In Vivo with a Cleavage-Specific Antibody." International Journal of Molecular Sciences, vol. 23, no. 8, 2022. https://doi.org/10.3390/ijms23084355

Fabris, Federico, Petra Šoštarić, Ivica Matak, Thomas Binz, Anna Toffan, Morena Simonato, Cesare Montecucco, Marco Pirazzini and Ornella Rossetto. "Detection of VAMP Proteolysis by Tetanus and Botulinum Neurotoxin Type B In Vivo with a Cleavage-Specific Antibody." International Journal of Molecular Sciences 23, no. 8 (2022). https://doi.org/10.3390/ijms23084355

Fabris, F., et al. (2022) 'Detection of VAMP Proteolysis by Tetanus and Botulinum Neurotoxin Type B In Vivo with a Cleavage-Specific Antibody', International Journal of Molecular Sciences, 23(8). doi: 10.3390/ijms23084355

Fabris F, Šoštarić P, Matak I, Binz T, Toffan A, Simonato M, and sur.. Detection of VAMP Proteolysis by Tetanus and Botulinum Neurotoxin Type B In Vivo with a Cleavage-Specific Antibody. International Journal of Molecular Sciences [Internet]. 2022 April 14 [cited 2024 December 22];23(8). doi: 10.3390/ijms23084355

F. Fabris, et al., "Detection of VAMP Proteolysis by Tetanus and Botulinum Neurotoxin Type B In Vivo with a Cleavage-Specific Antibody", International Journal of Molecular Sciences, vol. 23, no. 8, April 2022. [Online]. Available at: https://urn.nsk.hr/urn:nbn:hr:105:896979. [Accessed: 22 December 2024]

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Metadata
Title (english)Detection of VAMP Proteolysis by Tetanus and Botulinum Neurotoxin Type B In Vivo with a Cleavage-Specific Antibody
AuthorFederico Fabris
AuthorPetra Šoštarić
AuthorIvica Matak
AuthorThomas Binz
AuthorAnna Toffan
AuthorMorena Simonato
AuthorCesare Montecucco
AuthorMarco Pirazzini
AuthorOrnella Rossetto
Author's institutionUniversity of Zagreb
School of Medicine
(Department of Pharmacology)
Scientific / art field,
discipline and subdiscipline		BIOMEDICINE AND HEALTHCARE
Basic Medical Sciences
Pharmacology
Abstract (english)
Tetanus and Botulinum type B neurotoxins are bacterial metalloproteases that specifically cleave the vesicle-associated membrane protein VAMP at an identical peptide bond, resulting in inhibition of neuroexocytosis. The minute amounts of these neurotoxins commonly used in experimental animals are not detectable, nor is detection of their VAMP substrate sensitive enough. The immune detection of the cleaved substrate is much more sensitive, as we have previously shown for botulinum neurotoxin type A. Here, we describe the production in rabbit of a polyclonal antibody raised versus a peptide encompassing the 13 residues C-terminal with respect to the neurotoxin cleavage site. The antibody was affinity purified and found to recognize, with high specificity and selectivity, the novel N-terminus of VAMP that becomes exposed after cleavage by tetanus toxin and botulinum toxin type B. This antibody recognizes the neoepitope not only in native and denatured VAMP but also in cultured neurons and in neurons in vivo in neurotoxin-treated mice or rats, suggesting the great potential of this novel tool to elucidate tetanus and botulinum B toxin activity in vivo.
Keywords (english)
Languageenglish
Publication typeScientific paper - Original scientific paper
Publication statusPublished
Peer reviewPeer review
Publication versionPublished version
Journal titleInternational Journal of Molecular Sciences
Numberingvol. 23, no. 8, 4355
e-ISSN1422-0067
DOIhttps://doi.org/10.3390/ijms23084355
URN:NBNurn:nbn:hr:105:896979
Publication2022-04-14
Document URLhttps://www.mdpi.com/1422-0067/23/8/4355
Type of resourceText
Access conditionsOpen access
Terms of useLicence Attribution 4.0 International (CC BY 4.0) icon
Created on2022-12-12 08:17:47