prikaz prve stranice dokumenta Detection of VAMP Proteolysis by Tetanus and Botulinum Neurotoxin Type B In Vivo with a Cleavage-Specific Antibody
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Detection of VAMP Proteolysis by Tetanus and Botulinum Neurotoxin Type B In Vivo with a Cleavage-Specific Antibody
International Journal of Molecular Sciences, 23 (2022), 8; 4355. https://doi.org/10.3390/ijms23084355
Fabris, Federico; Šoštarić, Petra; Matak, Ivica; Binz, Thomas; Toffan, Anna; Simonato, Morena; Montecucco, Cesare; Pirazzini, Marco; Rossetto, Ornella

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Fabris, F., Šoštarić, P., Matak, I., Binz, T., Toffan, A., Simonato, M. ... Rossetto, O. (2022). Detection of VAMP Proteolysis by Tetanus and Botulinum Neurotoxin Type B In Vivo with a Cleavage-Specific Antibody. International Journal of Molecular Sciences, 23. (8). doi: 10.3390/ijms23084355

Fabris, Federico, et al. "Detection of VAMP Proteolysis by Tetanus and Botulinum Neurotoxin Type B In Vivo with a Cleavage-Specific Antibody." International Journal of Molecular Sciences, vol. 23, br. 8, 2022. https://doi.org/10.3390/ijms23084355

Fabris, Federico, Petra Šoštarić, Ivica Matak, Thomas Binz, Anna Toffan, Morena Simonato, Cesare Montecucco, Marco Pirazzini i Ornella Rossetto. "Detection of VAMP Proteolysis by Tetanus and Botulinum Neurotoxin Type B In Vivo with a Cleavage-Specific Antibody." International Journal of Molecular Sciences 23, br. 8 (2022). https://doi.org/10.3390/ijms23084355

Fabris, F., et al. (2022) 'Detection of VAMP Proteolysis by Tetanus and Botulinum Neurotoxin Type B In Vivo with a Cleavage-Specific Antibody', International Journal of Molecular Sciences, 23(8). doi: 10.3390/ijms23084355

Fabris F, Šoštarić P, Matak I, Binz T, Toffan A, Simonato M, i sur.. Detection of VAMP Proteolysis by Tetanus and Botulinum Neurotoxin Type B In Vivo with a Cleavage-Specific Antibody. International Journal of Molecular Sciences [Internet]. 14.04.2022. [pristupljeno 15.10.2024.];23(8). doi: 10.3390/ijms23084355

F. Fabris, et al., "Detection of VAMP Proteolysis by Tetanus and Botulinum Neurotoxin Type B In Vivo with a Cleavage-Specific Antibody", International Journal of Molecular Sciences, vol. 23, br. 8, Travanj 2022. [Online]. Dostupno na: https://urn.nsk.hr/urn:nbn:hr:105:896979. [Citirano: 15.10.2024.]

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Naslov (engleski)Detection of VAMP Proteolysis by Tetanus and Botulinum Neurotoxin Type B In Vivo with a Cleavage-Specific Antibody
AutorFederico Fabris
AutorPetra Šoštarić
AutorIvica Matak
AutorThomas Binz
AutorAnna Toffan
AutorMorena Simonato
AutorCesare Montecucco
AutorMarco Pirazzini
AutorOrnella Rossetto
Autorova ustanovaSveučilište u Zagrebu
Medicinski fakultet
(KATEDRA ZA TEMELJNU I KLINIČKU FARMAKOLOGIJU)
Znanstveno / umjetničko
područje, polje i grana		BIOMEDICINA I ZDRAVSTVO
Temeljne medicinske znanosti
Farmakologija
Sažetak (engleski)
Tetanus and Botulinum type B neurotoxins are bacterial metalloproteases that specifically cleave the vesicle-associated membrane protein VAMP at an identical peptide bond, resulting in inhibition of neuroexocytosis. The minute amounts of these neurotoxins commonly used in experimental animals are not detectable, nor is detection of their VAMP substrate sensitive enough. The immune detection of the cleaved substrate is much more sensitive, as we have previously shown for botulinum neurotoxin type A. Here, we describe the production in rabbit of a polyclonal antibody raised versus a peptide encompassing the 13 residues C-terminal with respect to the neurotoxin cleavage site. The antibody was affinity purified and found to recognize, with high specificity and selectivity, the novel N-terminus of VAMP that becomes exposed after cleavage by tetanus toxin and botulinum toxin type B. This antibody recognizes the neoepitope not only in native and denatured VAMP but also in cultured neurons and in neurons in vivo in neurotoxin-treated mice or rats, suggesting the great potential of this novel tool to elucidate tetanus and botulinum B toxin activity in vivo.
Ključne riječi (engleski)
Jezikengleski
Vrsta publikacijeZnanstveni rad - Izvorni znanstveni rad
Status objaveObjavljen
Vrsta recenzijeRecenziran
Verzija publikacijeObjavljena verzija rada (izdavačev PDF)
Naslov časopisaInternational Journal of Molecular Sciences
Brojčani podacivol. 23, br. 8, 4355
e-ISSN1422-0067
DOIhttps://doi.org/10.3390/ijms23084355
URN:NBNurn:nbn:hr:105:896979
Datum objave publikacije2022-04-14
URL dokumentahttps://www.mdpi.com/1422-0067/23/8/4355
Vrsta resursaTekst
Prava pristupaOtvoreni pristup
Uvjeti korištenjaIkona licence Imenovanje 4.0 međunarodna (CC BY 4.0)
Datum i vrijeme pohrane2022-12-12 08:17:47