prikaz prve stranice dokumenta Gangliosidome of a Human Hippocampus in Temporal Lobe Epilepsy Resolved by High-Resolution Tandem Mass Spectrometry
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Scientific paper - Original scientific paper
Gangliosidome of a Human Hippocampus in Temporal Lobe Epilepsy Resolved by High-Resolution Tandem Mass Spectrometry
Molecules, 27 (2022), 13; 4056. https://doi.org/10.3390/molecules27134056
Ica, Raluca; Mlinac-Jerković, Kristina; Ilić, Katarina; Sajko, Tomislav; Munteanu, Cristian V. A.; Zamfir, Alina D.; Kalanj-Bognar, Svjetlana

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Ica, R., Mlinac Jerković, K., Ilić, K., Sajko, T., Munteanu, C. V. A., Zamfir, A. D. & Kalanj Bognar, S. (2022). Gangliosidome of a Human Hippocampus in Temporal Lobe Epilepsy Resolved by High-Resolution Tandem Mass Spectrometry. Molecules, 27. (13). doi: 10.3390/molecules27134056

Ica, Raluca, et al. "Gangliosidome of a Human Hippocampus in Temporal Lobe Epilepsy Resolved by High-Resolution Tandem Mass Spectrometry." Molecules, vol. 27, no. 13, 2022. https://doi.org/10.3390/molecules27134056

Ica, Raluca, Kristina Mlinac Jerković, Katarina Ilić, Tomislav Sajko, Cristian V. A. Munteanu, Alina D. Zamfir and Svjetlana Kalanj Bognar. "Gangliosidome of a Human Hippocampus in Temporal Lobe Epilepsy Resolved by High-Resolution Tandem Mass Spectrometry." Molecules 27, no. 13 (2022). https://doi.org/10.3390/molecules27134056

Ica, R., et al. (2022) 'Gangliosidome of a Human Hippocampus in Temporal Lobe Epilepsy Resolved by High-Resolution Tandem Mass Spectrometry', Molecules, 27(13). doi: 10.3390/molecules27134056

Ica R, Mlinac Jerković K, Ilić K, Sajko T, Munteanu CVA, Zamfir AD, and sur.. Gangliosidome of a Human Hippocampus in Temporal Lobe Epilepsy Resolved by High-Resolution Tandem Mass Spectrometry. Molecules [Internet]. 2022 June 23 [cited 2024 April 25];27(13). doi: 10.3390/molecules27134056

R. Ica, et al., "Gangliosidome of a Human Hippocampus in Temporal Lobe Epilepsy Resolved by High-Resolution Tandem Mass Spectrometry", Molecules, vol. 27, no. 13, June 2022. [Online]. Available at: https://urn.nsk.hr/urn:nbn:hr:105:696067. [Accessed: 25 April 2024]

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Metadata
Title (english)Gangliosidome of a Human Hippocampus in Temporal Lobe Epilepsy Resolved by High-Resolution Tandem Mass Spectrometry
AuthorRaluca Ica
AuthorKristina Mlinac-Jerković
AuthorKatarina Ilić
AuthorTomislav Sajko
AuthorCristian V. A. Munteanu
AuthorAlina D. Zamfir
AuthorSvjetlana Kalanj-Bognar
Author's institutionUniversity of Zagreb
School of Medicine
(Department of Medical Chemistry, Biochemistry and Clinical Chemistry)
Scientific / art field,
discipline and subdiscipline		BIOMEDICINE AND HEALTHCARE
Clinical Medical Sciences
Abstract (english)
In this study, we developed a high-resolution tandem mass spectrometry (HR MS) approach to assess presumed changes in gangliosidome of a human hippocampus affected by temporal lobe epilepsy (TLE) in comparison with a normal hippocampus. Gangliosides, membrane glycolipids, are particularly diverse and abundant in the human brain, and participate in ion transport and modulation of neuronal excitability. Changes in structural ganglioside pattern potentially linked to TLE molecular pathogenesis have not been explored in detail. Aiming to characterize TLE-specific gangliosidome, we analyzed the native gangliosides purified from a human hippocampal tissue sample affected by TLE and a control hippocampus using HR MS. Marked differences of ganglioside expression were shown in TLE vs. control, particularly with respect to the sialylation degree of components, discovered as a characteristic feature of TLE. Another major finding is the occurrence of tetrasialofucogangliosides in TLE and species modified by either O-acetylation or CH3COO−. Structural analysis by higher-energy collisional dissociation (HCD) MS/MS gave rise to fragmentation patterns implying that the GQ1b (d18:1/18:0) isomer is specifically associated with TLE. Further investigation in a larger sample is needed in order to confirm the discovery of ganglioside structures specifically expressed in human TLE and to provide information on the probable role of gangliosides in the molecular events underlying seizures.
Keywords (english)
Languageenglish
Publication typeScientific paper - Original scientific paper
Publication statusPublished
Peer reviewPeer review
Publication versionPublished version
Journal titleMolecules
Numberingvol. 27, no. 13, 4056
e-ISSN1420-3049
DOIhttps://doi.org/10.3390/molecules27134056
URN:NBNurn:nbn:hr:105:696067
Publication2022-06-23
Document URLhttps://www.mdpi.com/1420-3049/27/13/4056
Type of resourceText
Access conditionsOpen access
Terms of useLicence Attribution 4.0 International (CC BY 4.0) icon
Created on2023-04-19 10:37:09