Morfološka analiza mišjih mozgova nakon ishemijske ozljede i primjene natriuretskih peptida

master's thesis

• Title (english): Morphological analysis of mice brain after ischemic stroke and natriuretic peptides application
• Author: Sven Šimunić
• Mentor: Srećko Gajović (mentor)
• Committee member: Aleksandra Sinđić (predsjednik povjerenstva)
• Committee member: Dinko Mitrečić (član povjerenstva)
• Committee member: Srećko Gajović (član povjerenstva)
• Granter: University of Zagreb; School of Medicine; (Department of Histology and Embryology); Zagreb
• Defense date and country: 2015-07-15, Croatia
• Scientific / art field, discipline and subdiscipline: BIOMEDICINE AND HEALTHCARE; Clinical Medical Sciences; Neurology

Abstract

Prekid opskrbe mozga krvlju zbog začepljenja moždanih arterija dovodi do moždanog udara te posljedično do moždanog edema koji pridonosi povećanju ishemijske ozljede. Vazogeni peptidi bradikinin i natriuretski peptidi imaju važnu ulogu u razvoju moždanog
edema nakon ishemijskog moždanog udara. Cilj ovog istraživanja bio je razjasniti učinak natriuretskih peptida, bradikinina te njihovog međudjelovanja u događajima koji slijede nakon ishemijske ozljede mozga. U in vivo pokusima na mišjem modelu moždanog udara pokazali smo da intravenska primjena urodilatina sprječava nastanak ishemijske lezije mozga s posljedičnim moždanim edemom, a koje smo određivali mjerenjem stupnja neurološkog oštećenja te mjerenjem volumena ishemijske lezije i postotka moždanog edema, kao i patohistološkom analizom na serijskim rezovima mozgova histološki bojenima po Nisslu.
Rezultati ovog istraživanja ukazuju na postojanje prirodnog antagonizma bradikininskog receptora tipa 2 te moguću primjenu natriuretskih peptida u liječenju moždanog udara.

Abstract (english)

Reduction in blood flow to the brain caused by the occlusion of cerebral arteries leads to stroke and consequential brain edema formation, which further increases the size of ischemic lesion. Vasogenic peptides bradykinin and natriuretic peptides play an important role in the formation of cerebral edema after ischemic stroke. The aim of this research was to clarify the possible effects of natriuretic peptides, bradykinin and their interactive effects on edema formation after ischemic brain injury. The in vivo experiments on a mouse model of stroke showed that urodilatin inhibited the formation of the ischemic lesion and brain edema, which was measured by neurological scoring, volumetric analysis of the ischemic lesion and
brain edema along with patohistological analysis of serial Nissl stained sections. The results of this research show the existence of a natural antagonism of the bradykinin receptor type 2, and imply the possible use of natriuretic peptides in treatment of stroke.

• Keywords: moždani udar; moždani edem; natriuretski peptid; bradikinin; in vivo pokus
• Keywords (english): stroke; brain edema; natriuretic peptides; bradykinin; in vivo experiments
• Language: croatian
• URN:NBN: urn:nbn:hr:105:383072
• Study programme: Title: Medicine; Study programme type: university; Study level: integrated undergraduate and graduate; Academic / professional title: doktor/doktorica medicine (doktor/doktorica medicine)
• Type of resource: Text
• File origin: Born digital
• Access conditions: Open access
• Public note: Ključne riječi izradio administrator.
• Created on: 2016-04-04 12:11:30