Uloga metaloproteinaza matriksa i njihovih inhibitora u razvoju vratnih metastaza papilarnoga karcinoma štitne žlijezde

doctoral thesis

• Title (english): Role of matrix metalloproteinases and their inhibitors in the development of cervical metastases in papillary thyroid cancer
• Author: Boris Bumber
• Mentor: Drago Prgomet (mentor)
• Committee member: nije dostupno (član povjerenstva)
• Granter: University of Zagreb; School of Medicine; Zagreb
• Defense date and country: 2018-04-20, Croatia
• Scientific / art field, discipline and subdiscipline: BIOMEDICINE AND HEALTHCARE; Clinical Medical Sciences
• Universal decimal classification (UDC): 61 - Medical sciences

Abstract

Papilarni karcinom najčešći je karcinom štitnjače s porastom incidencije u svijetu. Obilježen je visokim brojem vratnih metastaza. Istraživanja invazije i metastaziranja identificirala su metaloproteinaze matriksa (MMP) kao ključne enzime u procesima tumorske diseminacije, osobito MMP-1, -2 i -9. Aktivnost MMP-a mogu modulirati tkivni inhibitori metaloproteinaza matriksa (TIMP 1-4). Ovo je istraživanje retrospektivno i istražuje ekspresije MMP-1, -2 i -9 te TIMP-1 i -2 u tkivu papilarnog karcinoma štitnjače. Cilj istraživanja bio je imunohistokemijski utvrditi povezanost MMP-a i TIMP-a s obrascem metastaziranja papilarnog karcinoma. Dobiveni rezultati pokazuju da su najviše ekspresije MMP-1 i TIMP-1 bile kod bolesnika s metastazama u lateralnim regijama vrata, dok se ekspresije MMP-2, MMP-9 i TIMP-2 nisu statistički razlikovale ovisno o obrascu metastaziranja. Analizirajući bolesnike u skupinama s metastazama i bez njih uočila se povezanost ekspresije ponovno MMP-1 i TIMP-1 s metastatskom skupinom. Uspoređujući kliničko patološke značajke i ekspresiju, zabilježene su značajne pozitivne korelacije MMP-1 sa stadijem bolesti i brojem zahvaćenih limfnih čvorova, TIMP-1 s brojem limfnih čvorova i stadijem tumora te TIMP-2 s veličinom tumora i brojem limfnih čvorova. Sukladno ovom istraživanju pretpostavljamo da su ekspresije MMP-1 i TIMP-1 povezane s obrascem metastaziranja papilarnog karcinoma štitnjače, dok se razina ostalih analiziranih proteina ne povezuje s obrascem metastaziranja.

Abstract (english)

Papillary carcinoma is the most common thyroid cancer with the incidence increasing worldwide. It is characterized by a high number of cervical metastases. Research of the invasiveness and metastatic processes identified matrix metalloproteinases (MMP) as a key enzyme family in tumor progression, especially MMP-1, -2 and -9. MMP activity can be modulated by tissue inhibitors of matrix metalloproteinase (TIMP 1-4). This research retrospectively investigated the expression of MMP-1, -2 and -9, as well as TIMP-1 and -2 in papillary thyroid carcinoma tissue. The aim of the study was to determine the association of MMP and TIMP with papillary thyroid cancer metastasis pattern by immunohistochemistry. The results obtained showed highest expression of MMP-1 and TIMP-1 in cancer tissue of patients with metastasis in the lateral region, while MMP-2, MMP-9 and TIMP-2 did not differ statistically according to the metastasis pattern. Comparing clinical features with expression data, MMP-1 significantly correlated with the disease stage and number of positive lymph nodes, TIMP-1 with number of positive lymph nodes and tumor stage, and TIMP-2 with tumor size and number of positive lymph nodes. According to this study, MMP-1 and TIMP-1, unlike other proteins analysed, appear to be associated with the papillary thyroid cancer metastasis pattern

• Keywords: medicina
• Keywords (english): medicine
• Language: croatian
• URN:NBN: urn:nbn:hr:105:634836
• Study programme: Title: Biomedicine and Health Sciences; Study programme type: university; Study level: postgraduate; Academic / professional title: doktor/doktorica znanosti, područje biomedicine i zdravstva (doktor/doktorica znanosti, područje biomedicine i zdravstva)
• Type of resource: Text
• Extent: 76 str.
• File origin: Born digital
• Access conditions: Open access
• Created on: 2023-05-05 08:08:28