Testicular Germ Cell Tumor Tissue Biomarker Analysis: A Comparison of Human Protein Atlas and Individual Testicular Germ Cell Tumor Component Immunohistochemistry

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• Autor: Jure Krasić
• Autor: Lucija Škara Abramović
• Autor: Marta Himelreich Perić
• Autor: Vedran Vanjorek
• Autor: Marko Gangur
• Autor: Dragana Zovko
• Autor: Marina Malnar
• Autor: Silvija Mašić
• Autor: Alma Demirović
• Autor: Bernardica Jurić
• Autor: Monika Ulamec
• Autor: Marijana Ćorić
• Autor: Davor Ježek
• Autor: Tomislav Kuliš
• Autor: Nino Sinčić
• Autorova ustanova: Sveučilište u Zagrebu; Medicinski fakultet; (KATEDRA ZA HISTOLOGIJU I EMBRIOLOGIJU)
• Autorova ustanova: Sveučilište u Zagrebu; Medicinski fakultet; (KATEDRA ZA MEDICINSKU BIOLOGIJU)
• Autorova ustanova: Sveučilište u Zagrebu; Medicinski fakultet; (KATEDRA ZA PATOLOGIJU)
• Autorova ustanova: Sveučilište u Zagrebu; Medicinski fakultet; (KATEDRA ZA UROLOGIJU)
• Znanstveno / umjetničko područje, polje i grana: BIOMEDICINA I ZDRAVSTVO; Kliničke medicinske znanosti; Patologija
• Znanstveno / umjetničko područje, polje i grana: BIOMEDICINA I ZDRAVSTVO; Kliničke medicinske znanosti; Urologija
• Znanstveno / umjetničko područje, polje i grana: BIOMEDICINA I ZDRAVSTVO; Temeljne medicinske znanosti; Citologija, histologija i embriologija

Sažetak (engleski)

The accurate management of testicular germ cell tumors (TGCTs) depends on identifying the individual histological tumor components. Currently available data on protein expression in TGCTs are limited. The human protein atlas (HPA) is a comprehensive resource presenting the expression and localization of proteins across tissue types and diseases. In this study, we have compared the data from the HPA with our in-house immunohistochemistry on core TGCT diagnostic genes to test reliability and potential biomarker genes. We have compared the protein expression of 15 genes in TGCT patients and non-neoplastic testicles with the data from the HPA. Protein expression was converted into diagnostic positivity. Our study discovered discrepancies in three of the six core TGCT diagnostic genes, POU5F1, KIT and SOX17 in HPA. DPPA3, CALCA and TDGF1 were presented as potential novel TGCT biomarkers. MGMT was confirmed while RASSF1 and PRSS21 were identified as biomarkers of healthy testicular tissue. Finally, SALL4, SOX17, RASSF1 and PRSS21 dysregulation in the surrounding testicular tissue with complete preserved spermatogenesis of TGCT patients was detected, a potential early sign of neoplastic transformation. We highlight the importance of a multidisciplinary collaborative approach to fully understand the protein landscape of human testis and its pathologies.

• Ključne riječi (engleski): Biomarkers, Tumor / metabolism; Humans; Immunohistochemistry; Male; Neoplasms, Germ Cell and Embryonal / diagnosis; Neoplasms, Germ Cell and Embryonal / genetics; Reproducibility of Results; Testicular Neoplasms / diagnosis; Testicular Neoplasms / genetics; Testicular Neoplasms / pathology
• Jezik: engleski
• Vrsta publikacije: Znanstveni rad - Izvorni znanstveni rad
• Status objave: Objavljen
• Vrsta recenzije: Recenziran
• Verzija publikacije: Objavljena verzija rada (izdavačev PDF)
• Naslov časopisa: Cells
• Brojčani podaci: vol. 12, br. 14, 1841
• e-ISSN: 2073-4409
• DOI: https://doi.org/10.3390/cells12141841
• URN:NBN: urn:nbn:hr:105:014734
• Datum objave publikacije: 2023-07-13
• Projekt: Šifra: KK.01.1.1.01.0008; Naziv (hrvatski): Reproduktivna i regenerativna medicina - istraživanja novih platformi i potencijala; Naziv (engleski): Scientific Center of Excellence for Reproductive and Regenerative Medicine; Kratica: CERRM; Voditelj: Davor Ježek; Pravna nadležnost: eu; Financijer: Europska komisija; Linija financiranja: European Regional Development Fund
• URL dokumenta: https://www.mdpi.com/2073-4409/12/14/1841
• Vrsta resursa: Tekst
• Prava pristupa: Otvoreni pristup
• Datum i vrijeme pohrane: 2024-09-05 08:07:35