diplomski rad
MORPHOLOGICAL ANALYSIS OF BRAIN INJURY AFTER EARLY NEONATAL HYPOXIA - ISCHEMIA IN MICE

Gabriel David Borden (2016)
Sveučilište u Zagrebu
Medicinski fakultet
Podaci o radu
NaslovMORPHOLOGICAL ANALYSIS OF BRAIN INJURY AFTER EARLY NEONATAL HYPOXIA - ISCHEMIA IN MICE
AutorGabriel David Borden
Voditelj/MentorMarina Dobrivojević (mentor)
Sažetak rada
Perinatal ischemia is a common cause of cerebral palsy and other human morbidity. It is a growing problem in preterm infants in whom it is increasingly common because modern standards of care result in a greater number of infants who survive prematurity to experience ischemic brain pathology. In excess of 50% of babies born at 24 weeks currently enjoy longterm survival. Many neurodevelopmental processes in newborn mice correspond to this period in human development. Towards a better model of hypoxic/ischemic events in early human premature parturition, an implementation of the Rice-Vannucci model of perinatal ischemia in zero day old mice is therefore presented herein. The Rice-Vannucci (RV) model entails unilateral ligation of the common catid artery followed by a period of relative hypoxia, applied to neonatal rats or mice. Previously the youngest animals reported with this model were three day old mice, and one day old rats. Towards a better model of hypoxic-ischemic events in early human premature parturition, an implementation of the Rice-Vannucci (R-V) model of perinatal ischemia in zero day old mice is therefore presented herein. The modifications of the RV model entail unilateral ligation and interruption of the common carotid artery followed by a period of relative hypoxia, applied to postnatal day zero mice. Multiple imaging modalities were employed in the analysis, including magnetic resonance imaging (MRI), histological Nissl staining, as well as immunohistochemical staining of exvivo day three and seven postnatal mice brains. Together, comparing diffusion-weighted and T2-weighted MRI to Nissl staining data confirm the successful implementation of the R-V model, establishing a foundation for follow-up investigations.
Ključne riječiperinatal ischemia Rice-Vannucci neonatal
Povjerenstvo za obranuDinko Mitrečić (predsjednik povjerenstva)
Marina Dobrivojević (član povjerenstva)
Aleksandra Dugandžić (član povjerenstva)
Ustanova koja je dodijelila akademski/stručni stupanjSveučilište u Zagrebu
Medicinski fakultet
MjestoZagreb
Država obraneHrvatska
Znanstveno područje, polje, granaBIOMEDICINA I ZDRAVSTVO
Kliničke medicinske znanosti
Neurologija
Vrsta studijasveučilišni
Stupanjintegrirani preddiplomski i diplomski
Naziv studijskog programaMedicina (na engleskom jeziku)
Akademski / stručni nazivdoktor/doktorica medicine
Kratica akademskog / stručnog nazivadr. med.
Vrsta radadiplomski rad
Jezik engleski
Datum obrane2016-07-15
Vrsta resursatekst
Prava pristupaRad u otvorenom pristupu
Uvjeti korištenja radahttp://rightsstatements.org/vocab/InC/1.0/
URN:NBNhttps://urn.nsk.hr/urn:nbn:hr:105:607701
PohranioMarijan Šember