The effect of pentadecapeptide BPC 157 on hippocampal ischemia/reperfusion injuries in rats

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• Autor: Jakša Vukojević
• Autor: Borna Vrdoljak
• Autor: Dominik Malekinušić
• Autor: Marko Siroglavić
• Autor: Marija Milavić
• Autor: Danijela Kolenc
• Autor: Alenka Boban Blagaić
• Autor: Lovorka Batelja
• Autor: Domagoj Drmić
• Autor: Sven Seiwerth
• Autor: Predrag Sikirić
• Autorova ustanova: Sveučilište u Zagrebu; Medicinski fakultet; (KATEDRA ZA TEMELJNU I KLINIČKU FARMAKOLOGIJU)
• Autorova ustanova: Sveučilište u Zagrebu; Medicinski fakultet; (KATEDRA ZA PATOLOGIJU)
• Znanstveno / umjetničko područje, polje i grana: BIOMEDICINA I ZDRAVSTVO; Kliničke medicinske znanosti; Patologija
• Znanstveno / umjetničko područje, polje i grana: BIOMEDICINA I ZDRAVSTVO; Temeljne medicinske znanosti; Farmakologija

Sažetak (engleski)

Background and purpose: We focused on the, yet undescribed, therapy effect of the stable gastric pentadecapeptide BPC 157 in hippocampal ischemia/reperfusion injuries, after bilateral clamping of the common carotid arteries in rats. The background is the proven therapy effect of BPC 157 in ischemia/reperfusion injuries in different tissues. Furthermore, there is the subsequent oxidative stress counteraction, particularly when given during reperfusion. The recovering effect it has on occluded... Više vessels, results with activation of the alternative pathways, bypassing the occlusion in deep vein thrombosis. Finally, the BPC 157 therapy benefits with its proposed role as a novel mediator of Roberts' cytoprotection and bidirectional effects in the gut-brain axis.
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Materials and methods: Male Wistar rats underwent bilateral clamping of the common carotid arteries for a 20-min period. At 30 s thereafter, we applied medication (BPC 157 10 µg/kg; or saline) as a 1 ml bath directly to the operated area, that is, trigonum caroticum. We documented, in reperfusion, the resolution of the neuronal damages sustained in the brain, resolution of the damages reflected in memory, locomotion, and coordination disturbances, with the presentation of the particular genes expression in hippocampal tissues.
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Results: In the operated rats, at 24 and 72 hr of the reperfusion, the therapy counteracted both early and delayed neural hippocampal damage, achieving full functional recovery (Morris water maze test, inclined beam-walking test, lateral push test). mRNA expression studies at 1 and 24 hr, provided strongly elevated (Egr1, Akt1, Kras, Src, Foxo, Srf, Vegfr2, Nos3, and Nos1) and decreased (Nos2, Nfkb) gene expression (Mapk1 not activated), as a way how BPC 157 may act.
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Conclusion: Together, these findings suggest that these beneficial BPC 157 effects may provide a novel therapeutic solution for stroke. Sakrij dio sažetka

• Ključne riječi (engleski): BPC 157; cytoprotection; hippocampus; ischemia; ischemia injuries; nitric oxide; peptide; reperfusion
• Jezik: engleski
• Vrsta publikacije: Znanstveni rad - Izvorni znanstveni rad
• Status objave: Objavljen
• Vrsta recenzije: Recenziran
• Verzija publikacije: Objavljena verzija rada (izdavačev PDF)
• Naslov časopisa: Brain and Behavior
• Brojčani podaci: vol. 10, br. 8, e01726
• p-ISSN: 2162-3279
• e-ISSN: 2162-3279
• DOI: https://doi.org/10.1002/brb3.1726
• URN:NBN: urn:nbn:hr:105:910671
• Datum objave publikacije: 2020-08
• URL dokumenta: https://onlinelibrary.wiley.com/doi/10.1002/brb3.1726
• Vrsta resursa: Tekst
• Prava pristupa: Otvoreni pristup
• Datum i vrijeme pohrane: 2021-03-25 08:10:06