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Comparison of indirect immunofluorescence and western blot method in the diagnosis of hantavirus infections
World Journal of Methodology, 11 (2021), 6; 294-301. https://doi.org/10.5662/wjm.v11.i6.294
Vilibić-Čavlek, Tatjana; Barbić, Ljubo; Stevanović, Vladimir; Savić, Vladimir; Mrzljak, Anna; Bogdanić, Maja; Tabain, Irena

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Vilibić Čavlek, T., Barbić, Lj., Stevanović, V., Savić, V., Mrzljak, A., Bogdanić, M. i Tabain, I. (2021). Comparison of indirect immunofluorescence and western blot method in the diagnosis of hantavirus infections. World Journal of Methodology, 11. (6), 294-301. doi: 10.5662/wjm.v11.i6.294

Vilibić Čavlek, Tatjana, et al. "Comparison of indirect immunofluorescence and western blot method in the diagnosis of hantavirus infections." World Journal of Methodology, vol. 11, br. 6, 2021, str. 294-301. https://doi.org/10.5662/wjm.v11.i6.294

Vilibić Čavlek, Tatjana, Ljubo Barbić, Vladimir Stevanović, Vladimir Savić, Anna Mrzljak, Maja Bogdanić i Irena Tabain. "Comparison of indirect immunofluorescence and western blot method in the diagnosis of hantavirus infections." World Journal of Methodology 11, br. 6 (2021): 294-301. https://doi.org/10.5662/wjm.v11.i6.294

Vilibić Čavlek, T., et al. (2021) 'Comparison of indirect immunofluorescence and western blot method in the diagnosis of hantavirus infections', World Journal of Methodology, 11(6), str. 294-301. doi: 10.5662/wjm.v11.i6.294

Vilibić Čavlek T, Barbić Lj, Stevanović V, Savić V, Mrzljak A, Bogdanić M, i sur.. Comparison of indirect immunofluorescence and western blot method in the diagnosis of hantavirus infections. World Journal of Methodology [Internet]. 20.11.2021. [pristupljeno 05.10.2024.];11(6):294-301. doi: 10.5662/wjm.v11.i6.294

T. Vilibić Čavlek, et al., "Comparison of indirect immunofluorescence and western blot method in the diagnosis of hantavirus infections", World Journal of Methodology, vol. 11, br. 6, str. 294-301, Studeni 2021. [Online]. Dostupno na: https://urn.nsk.hr/urn:nbn:hr:105:391304. [Citirano: 05.10.2024.]

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Podaci o radu
Naslov (engleski)Comparison of indirect immunofluorescence and western blot method in the diagnosis of hantavirus infections
AutorTatjana Vilibić-Čavlek
AutorLjubo Barbić
AutorVladimir Stevanović
AutorVladimir Savić
AutorAnna Mrzljak
AutorMaja Bogdanić
AutorIrena Tabain
Autorova ustanovaSveučilište u Zagrebu
Medicinski fakultet
(KATEDRA ZA INTERNU MEDICINU)
Autorova ustanovaSveučilište u Zagrebu
Medicinski fakultet
(KATEDRA ZA MEDICINSKU MIKROBIOLOGIJU I PARAZITOLOGIJU)
Znanstveno / umjetničko
područje, polje i grana		BIOMEDICINA I ZDRAVSTVO
Kliničke medicinske znanosti
Medicinska mikrobiologija
Znanstveno / umjetničko
područje, polje i grana		BIOMEDICINA I ZDRAVSTVO
Kliničke medicinske znanosti
Interna medicina
Sažetak (engleski)
Background: Serologic cross-reactivity between hantaviruses often complicates the interpretation of the results. ----- Aim: To analyze the diagnostic value of indirect immunofluorescence assay (IFA) and western blot (WB) in the diagnosis of hantavirus infections. ----- Methods: One hundred eighty-eight serum samples from Puumala (PUUV) and Dobrava (DOBV) orthohantavirus infected patients were analyzed. Serology was performed using commercial tests (Euroimmun, Lübeck, Germany). ----- Results: Using IFA, 49.5% of acute-phase samples showed a monotypic response to PUUV, while 50.5% cross-reacted with other hantaviruses. The overall cross-reactivity was higher for immunoglobulin G (IgG) (50.0%) than for immunoglobulin M (IgM) (25.5%). PUUV IgM/IgG antibodies showed low/moderate reactivity with orthohantaviruses Hantaan (12.3%/31.5%), Seoul (7.5%/17.8%), DOBV (5.4%/ 28.1%), and Saaremaa (4.8%/15.7%). Both DOBV IgM and IgG antibodies were broadly reactive with Hantaan (76.2%/95.2%), Saaremaa (80.9%/83.3%), and Seoul (78.6%/85.7%) and moderate with PUUV (28.5%/38.1%). Using a WB, serotyping was successful in most cross-reactive samples (89.5%). ----- Conclusion: The presented results indicate that WB is more specific than IFA in the diagnosis of hantavirus infections, confirming serotype in most IFA cross-reactive samples.
Ključne riječi (engleski)
Jezikengleski
Vrsta publikacijeZnanstveni rad - Izvorni znanstveni rad
Status objaveObjavljen
Vrsta recenzijeRecenziran
Verzija publikacijeObjavljena verzija rada (izdavačev PDF)
Naslov časopisaWorld Journal of Methodology
Brojčani podacivol. 11, br. 6, str. 294-301
p-ISSN2222-0682
DOIhttps://doi.org/10.5662/wjm.v11.i6.294
URN:NBNurn:nbn:hr:105:391304
Datum objave publikacije2021-11-20
URL dokumentahttps://www.wjgnet.com/2222-0682/full/v11/i6/294.htm
Vrsta resursaTekst
Prava pristupaOtvoreni pristup
Uvjeti korištenjaIkona licence Imenovanje-Nekomercijalno 4.0 međunarodna (CC BY-NC 4.0)
Datum i vrijeme pohrane2022-02-03 09:17:54