Diversified Effects of COVID-19 as a Consequence of the Differential Metabolism of Phospholipids and Lipid Peroxidation Evaluated in the Plasma of Survivors and Deceased Patients upon Admission to the Hospital

Znanstveni rad - Izvorni znanstveni rad

• Autor: Neven Žarković
• Autor: Wojciech Łuczaj
• Autor: Iwona Jarocka-Karpowicz
• Autor: Biserka Orehovec
• Autor: Bruno Baršić
• Autor: Marko Tarle
• Autor: Marta Kmet
• Autor: Ivica Lukšić
• Autor: Michał Biernacki
• Autor: Elżbieta Skrzydlewska
• Autorova ustanova: Sveučilište u Zagrebu; Medicinski fakultet; (KATEDRA ZA KIRURGIJU)
• Znanstveno / umjetničko područje, polje i grana: BIOMEDICINA I ZDRAVSTVO; Kliničke medicinske znanosti; Kirurgija

Sažetak (engleski)

As a result of SARS-CoV-2 infection, inflammation develops, which promotes oxidative stress, leading to modification of phospholipid metabolism. Therefore, the aim of this study is to compare the effects of COVID-19 on the levels of phospholipid and free polyunsaturated fatty acids (PUFAs) and their metabolites produced in response to reactions with reactive oxygen species (ROS) and enzymes (cyclooxygenases-(COXs) and lipoxygenase-(LOX)) in the plasma of patients who either recovered or passed away within a week of hospitalization. In the plasma of COVID-19 patients, especially of the survivors, the actions of ROS and phospholipase A2 (PLA2) cause a decrease in phospholipid fatty acids level and an increase in free fatty acids (especially arachidonic acid) despite increased COXs and LOX activity. This is accompanied by an increased level in lipid peroxidation products (malondialdehyde and 8-isoprostaglandin F2α) and lipid mediators generated by enzymes. There is also an increase in eicosanoids, both pro-inflammatory as follows: thromboxane B2 and prostaglandin E2, and anti-inflammatory as follows: 15-deoxy-Δ-12,14-prostaglandin J2 and 12-hydroxyeicosatetraenoic acid, as well as endocannabinoids (anandamide-(AEA) and 2-arachidonylglycerol-(2-AG)) observed in the plasma of patients who recovered. Moreover, the expression of tumor necrosis factor α and interleukins (IL-6 and IL-10) is increased in patients who recovered. However, in the group of patients who died, elevated levels of N-oleoylethanolamine and N-palmitoylethanolamine are found. Since lipid mediators may have different functions depending on the onset of pathophysiological processes, a stronger pro-inflammatory response in patients who have recovered may be the result of the defensive response to SARS-CoV-2 in survivors associated with specific changes in the phospholipid metabolism, which could also be considered a prognostic factor.

• Ključne riječi (engleski): Arachidonic Acids / metabolism; COVID-19; Dinoprostone / metabolism; Eicosanoids / metabolism; Endocannabinoids / metabolism; Fatty Acids, Nonesterified; Hospitalization; Hospitals; Humans; Hydroxyeicosatetraenoic Acids; Interleukin-10 / metabolism; Interleukin-6 / metabolism; Lipid Peroxidation; Lipoxygenase / metabolism; Malondialdehyde; Phospholipases A2 / metabolism; Phospholipids / metabolism; Prostaglandin-Endoperoxide Synthases / metabolism; Reactive Oxygen Species / metabolism; SARS-CoV-2; Survivors; Thromboxane B2; Tumor Necrosis Factor-alpha / metabolism
• Jezik: engleski
• Vrsta publikacije: Znanstveni rad - Izvorni znanstveni rad
• Status objave: Objavljen
• Vrsta recenzije: Recenziran
• Verzija publikacije: Objavljena verzija rada (izdavačev PDF)
• Naslov časopisa: International Journal of Molecular Sciences
• Brojčani podaci: vol. 23, br. 19, 11810
• e-ISSN: 1422-0067
• DOI: https://doi.org/10.3390/ijms231911810
• URN:NBN: urn:nbn:hr:105:371575
• Datum objave publikacije: 2022-10-05
• URL dokumenta: https://www.mdpi.com/1422-0067/23/19/11810
• Vrsta resursa: Tekst
• Prava pristupa: Otvoreni pristup
• Datum i vrijeme pohrane: 2023-08-22 08:40:04