Levels of selected oxidative stress markers in the vitreous and serum of diabetic retinopathy patients

Znanstveni rad - Izvorni znanstveni rad

• Autor: Vlatka Brzović-Šarić
• Autor: Irena Landeka
• Autor: Borna Šarić
• Autor: Monika Barberić
• Autor: Lidija Andrijašević
• Autor: Branimir Cerovski
• Autor: Nada Oršolić
• Autor: Domagoj Đikić
• Autorova ustanova: Sveučilište u Zagrebu; Medicinski fakultet
• Znanstveno / umjetničko područje, polje i grana: BIOMEDICINA I ZDRAVSTVO; Kliničke medicinske znanosti

Sažetak (engleski)

PURPOSE

In diabetes, an impaired antioxidant defense system contributes to the development of diabetic retinopathy. The main objective of this paper was to find correlations of oxidative stress parameters within and between the vitreous and serum in patients with type 2 diabetes who had developed proliferative diabetic retinopathy. ----- METHODS

The study included and compared two groups of patients who underwent vitrectomy: 37 patients with type 2 diabetes and proliferative retinopathy (PDR), and 50 patients with non-diabetic eye disorders (NDED). Vascular endothelial growth factor (VEGF), advanced oxidized protein product (AOPP), and oxidative stress markers (direct lipid hydroperoxidation (LPO), malondialdehyde (MDA), total superoxide dismutase (SOD), and glutathione (GSH)) were measured in the vitreous and serum of both groups and correlated with one another, between humoral compartments and with gender, age, and serum glucose levels. ----- RESULTS

In the vitreous of PDR patients, VEGF, LPO, and MDA (p<0.05) were increased and SOD values were slightly lowered (p<0.05) than in NDED patients. Vitreous AOPP and GSH showed no differences between the groups. In the serum, AOPP, MDA, and SOD were increased (p<0.05) and VEGF was slightly increased (p<0.05) in the PDR group compared to NDED. With regard to gender, similar changes were recorded for both groups, except for the lower serum MDA in males than females in the NDED group. Advanced age showed no significant effect on changes of measured parameters in the vitreous. In the serum, VEGF was positively correlated (p<0.05) and MDA and SOD negatively correlated (p<0.05) with increasing age. Among measured parameters within and between the vitreous and serum, several correlative links occurred in the PDR group that were not present in the NDED group. The most prominent correlation changes were between serum LPO and vitreal LPO, serum SOD and vitreal LPO, serum LPO and serum SOD, and vitreal VEGF and serum SOD. ----- CONCLUSIONS

Among the selected oxidative stress markers, SOD and LPO were highly correlative in both the vitreous and serum in PDR compared to patients without metabolic disorders. Their correlations suggested that monitoring their mutual alterations might be informative during PDR development and should be considered in further research.

• Ključne riječi (engleski): Advanced Oxidation Protein Products / blood; Advanced Oxidation Protein Products / metabolism; Aged; Aged, 80 and over; Biomarkers / blood; Biomarkers / metabolism; Blood Glucose / metabolism; Case-Control Studies; Diabetes Mellitus, Type 2 / blood; Diabetes Mellitus, Type 2 / metabolism; Diabetic Retinopathy / blood; Diabetic Retinopathy / metabolism; Female; Glutathione / blood; Glutathione / metabolism; Humans; Lipid Peroxides / blood; Lipid Peroxides / metabolism; Male; Malondialdehyde / blood; Malondialdehyde / metabolism; Middle Aged; Oxidative Stress; Superoxide Dismutase / blood; Superoxide Dismutase / metabolism; Vascular Endothelial Growth Factor A / blood; Vascular Endothelial Growth Factor A / metabolism; Vitreous Body / metabolism
• Jezik: engleski
• Vrsta publikacije: Znanstveni rad - Izvorni znanstveni rad
• Status objave: Objavljen
• Vrsta recenzije: Recenziran
• Verzija publikacije: Objavljena verzija rada (izdavačev PDF)
• Naslov časopisa: Molecular Vision
• Brojčani podaci: vol. 21, str. 649-664
• p-ISSN: 1090-0535
• URN:NBN: urn:nbn:hr:105:774923
• Datum objave publikacije: 2015-06-12
• URL dokumenta: http://www.molvis.org/molvis/v21/649
• Vrsta resursa: Tekst
• Prava pristupa: Otvoreni pristup
• Datum i vrijeme pohrane: 2024-03-28 08:09:49