VE-cadherin facilitates BMP-induced endothelial cell permeability and signaling

Znanstveni rad - Izvorni znanstveni rad

• Autor: Andreas Benn
• Autor: Clara Bredow
• Autor: Isabel Casanova
• Autor: Slobodan Vukičević
• Autor: Petra Knaus
• Autorova ustanova: Sveučilište u Zagrebu; Medicinski fakultet
• Znanstveno / umjetničko područje, polje i grana: BIOMEDICINA I ZDRAVSTVO; Temeljne medicinske znanosti

Sažetak (engleski)

Several vascular disorders, such as aberrant angiogenesis, atherosclerosis and pulmonary hypertension, have been linked to dysfunctional BMP signaling. Vascular hyperpermeability via distortion of endothelial cell adherens junctions is a common feature of these diseases, but the role of BMPs in this process has not been investigated. BMP signaling is initiated by binding of ligand to, and activation of, BMP type I (BMPRI) and type II (BMPRII) receptors. Internalization of VE-cadherin as well as c-Src kinase-dependent phosphorylation have been implicated in the loosening of cell-cell contacts, thereby modulating vascular permeability. Here we demonstrate that BMP6 induces hyperpermeabilization of human endothelial cells by inducing internalization and c-Src-dependent phosphorylation of VE-cadherin. Furthermore, we show BMP-dependent physical interaction of VE-cadherin with the BMP receptor ALK2 (BMPRI) and BMPRII, resulting in stabilization of the BMP receptor complex and, thereby, the support of BMP6-Smad signaling. Our results provide first insights into the molecular mechanism of BMP-induced vascular permeability, a hallmark of various vascular diseases, and provide the basis for further investigations of BMPs as regulators of vascular integrity, both under physiological and pathophysiological conditions.

• Ključne riječi (engleski): Adherens Junctions / drug effects; Adherens Junctions / metabolism; Antigens, CD / metabolism; Bone Morphogenetic Protein 6 / pharmacology; Bone Morphogenetic Protein Receptors / metabolism; Cadherins / metabolism; Capillary Permeability / drug effects; Cell Membrane Permeability / drug effects; Endocytosis / drug effects; Human Umbilical Vein Endothelial Cells / cytology; Human Umbilical Vein Endothelial Cells / drug effects; Human Umbilical Vein Endothelial Cells / metabolism; Humans; Models, Biological; Phosphorylation / drug effects; Phosphotyrosine / metabolism; Protein Binding / drug effects; Proto-Oncogene Proteins pp60(c-src) / metabolism; Signal Transduction / drug effects
• Jezik: engleski
• Vrsta publikacije: Znanstveni rad - Izvorni znanstveni rad
• Status objave: Objavljen
• Vrsta recenzije: Recenziran
• Verzija publikacije: Objavljena verzija rada (izdavačev PDF)
• Naslov časopisa: Journal of Cell Science
• Brojčani podaci: vol. 129, br. 1, str. 206-218
• p-ISSN: 0021-9533
• DOI: https://doi.org/10.1242/jcs.179960
• URN:NBN: urn:nbn:hr:105:220543
• Datum objave publikacije: 2016-01-01
• URL dokumenta: https://doi.org/10.1242/jcs.179960
• Vrsta resursa: Tekst
• Prava pristupa: Otvoreni pristup
• Datum i vrijeme pohrane: 2024-03-28 08:33:42