prikaz prve stranice dokumenta Impact of 5-Azacytidine on placental weight, glycoprotein pattern and proliferating cell nuclear antigen expression in rat placenta
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Impact of 5-Azacytidine on placental weight, glycoprotein pattern and proliferating cell nuclear antigen expression in rat placenta
Placenta, 28 (2007), 8-9; 803-811. https://doi.org/10.1016/j.placenta.2007.04.001
Šerman, Ljiljana; Vlahović, Maja; Šijan, Milan; Bulić-Jakuš, Floriana; Šerman, Alan; Sinčić, Nino; Matijević, Ratko; Jurić-Lekić, Gordana; Katušić, Ana

Citirajte ovaj rad

Šerman, Lj., Vlahović, M., Šijan, M., Bulić Jakuš, F., Šerman, A., Sinčić, N. ... Katušić, A. (2007). Impact of 5-Azacytidine on placental weight, glycoprotein pattern and proliferating cell nuclear antigen expression in rat placenta. Placenta, 28. (8-9), 803-811. doi: 10.1016/j.placenta.2007.04.001

Šerman, Ljiljana, et al. "Impact of 5-Azacytidine on placental weight, glycoprotein pattern and proliferating cell nuclear antigen expression in rat placenta." Placenta, vol. 28, br. 8-9, 2007, str. 803-811. https://doi.org/10.1016/j.placenta.2007.04.001

Šerman, Ljiljana, Maja Vlahović, Milan Šijan, Floriana Bulić Jakuš, Alan Šerman, Nino Sinčić, Ratko Matijević, Gordana Jurić Lekić i Ana Katušić. "Impact of 5-Azacytidine on placental weight, glycoprotein pattern and proliferating cell nuclear antigen expression in rat placenta." Placenta 28, br. 8-9 (2007): 803-811. https://doi.org/10.1016/j.placenta.2007.04.001

Šerman, Lj., et al. (2007) 'Impact of 5-Azacytidine on placental weight, glycoprotein pattern and proliferating cell nuclear antigen expression in rat placenta', Placenta, 28(8-9), str. 803-811. doi: 10.1016/j.placenta.2007.04.001

Šerman Lj, Vlahović M, Šijan M, Bulić Jakuš F, Šerman A, Sinčić N, i sur.. Impact of 5-Azacytidine on placental weight, glycoprotein pattern and proliferating cell nuclear antigen expression in rat placenta. Placenta [Internet]. 2007. [pristupljeno 27.10.2024.];28(8-9):803-811. doi: 10.1016/j.placenta.2007.04.001

Lj. Šerman, et al., "Impact of 5-Azacytidine on placental weight, glycoprotein pattern and proliferating cell nuclear antigen expression in rat placenta", Placenta, vol. 28, br. 8-9, str. 803-811, 2007. [Online]. Dostupno na: https://urn.nsk.hr/urn:nbn:hr:105:258213. [Citirano: 27.10.2024.]

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Naslov (engleski)Impact of 5-Azacytidine on placental weight, glycoprotein pattern and proliferating cell nuclear antigen expression in rat placenta
AutorLjiljana Šerman
AutorMaja Vlahović
AutorMilan Šijan
AutorFloriana Bulić-Jakuš
AutorAlan Šerman
AutorNino Sinčić
AutorRatko Matijević
AutorGordana Jurić-Lekić
AutorAna Katušić
Autorova ustanovaSveučilište u Zagrebu
Medicinski fakultet
Znanstveno / umjetničko
područje, polje i grana		BIOMEDICINA I ZDRAVSTVO
Kliničke medicinske znanosti
Sažetak (engleski)
During the placentation process, the expression of various glycoproteins plays an important role in embryonal development. Alterations in DNA methylation caused by 5-azacytidine (5azaC) can disturb normal glycoprotein expression as well as the proliferative ability of trophoblast cells. In order to assess this, a single dose of 5azaC was injected intraperitoneally into pregnant rats during days 1-19 of gestation. Animals were euthanised on day 20 and placental weight, as well as glycoprotein composition, was analysed together with immunohistological assessment of the degree of proliferation of the trophoblast cells. The placental weight was found to be significantly smaller in animals treated by 5azaC during days 4 to 14 of gestation (p<0.01, Student's t-test). The treatment on days 4, 5, and 6 resulted in a lack of labyrinth with the strong proliferative activity of the cells in the basal layer. Expression of glycoproteins with molecular mass smaller than 60kDa was reduced with treatment on day 6. The 5azaC administered from days 7 to 10 completely disturbed the placental structure and the proliferation of trophoblast cells was poor. During these days GP70 exhibited stronger expression in treated animals, contrary to GP40, which was stronger in controls. A natural border between the labyrinth and the basal layer was established on days 11 and 12. The basal layer was dominant with a lower proliferation of trophoblast cells compared with the controls. With the establishment of the labyrinth on day 13, the expression of GP40 was restored. Proliferation of the trophoblast cells from days 13 to 15 was higher compared with the controls. The changes in placental mass and the proliferative ability of trophoblast cells in rat placenta exposed to 5azaC represent more proof of the importance of epigenetics in the regulation of placental development.
Ključne riječi (engleski)
Jezikengleski
Vrsta publikacijeZnanstveni rad - Izvorni znanstveni rad
Status objaveObjavljen
Vrsta recenzijeRecenziran
Verzija publikacijeZavršna verzija rukopisa prihvaćena za objavljivanje (postprint)
Naslov časopisaPlacenta
Brojčani podacivol. 28, br. 8-9, str. 803-811
p-ISSN0143-4004
DOIhttps://doi.org/10.1016/j.placenta.2007.04.001
URN:NBNurn:nbn:hr:105:258213
Datum objave publikacije2007
URL dokumentahttps://doi.org/10.1016/j.placenta.2007.04.001
Vrsta resursaTekst
Prava pristupaOtvoreni pristup
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Datum i vrijeme pohrane2024-03-28 08:49:31