prikaz prve stranice dokumenta Preliminary Comparison of Molecular Antioxidant and Inflammatory Mechanisms Determined in the Peripheral Blood Granulocytes of COVID-19 Patients
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Preliminary Comparison of Molecular Antioxidant and Inflammatory Mechanisms Determined in the Peripheral Blood Granulocytes of COVID-19 Patients
International Journal of Molecular Sciences, 24 (2023), 17; 13574. https://doi.org/10.3390/ijms241713574
Skrzydlewska, Elżbieta; Łuczaj, Wojciech; Biernacki, Michał; Wójcik, Piotr; Jarocka-Karpowicz, Iwona; Orehovec, Biserka; Baršić, Bruno; Tarle, Marko; Kmet, Marta; Lukšić, Ivica; Marušić, Zlatko; Bauer, Georg; Žarković, Neven

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Skrzydlewska, E., Łuczaj, W., Biernacki, M., Wójcik, P., Jarocka Karpowicz, I., Orehovec, B. ... Žarković, N. (2023). Preliminary Comparison of Molecular Antioxidant and Inflammatory Mechanisms Determined in the Peripheral Blood Granulocytes of COVID-19 Patients. International Journal of Molecular Sciences, 24. (17). doi: 10.3390/ijms241713574

Skrzydlewska, Elżbieta, et al. "Preliminary Comparison of Molecular Antioxidant and Inflammatory Mechanisms Determined in the Peripheral Blood Granulocytes of COVID-19 Patients." International Journal of Molecular Sciences, vol. 24, br. 17, 2023. https://doi.org/10.3390/ijms241713574

Skrzydlewska, Elżbieta, Wojciech Łuczaj, Michał Biernacki, Piotr Wójcik, Iwona Jarocka Karpowicz, Biserka Orehovec, Bruno Baršić, et al. "Preliminary Comparison of Molecular Antioxidant and Inflammatory Mechanisms Determined in the Peripheral Blood Granulocytes of COVID-19 Patients." International Journal of Molecular Sciences 24, br. 17 (2023). https://doi.org/10.3390/ijms241713574

Skrzydlewska, E., et al. (2023) 'Preliminary Comparison of Molecular Antioxidant and Inflammatory Mechanisms Determined in the Peripheral Blood Granulocytes of COVID-19 Patients', International Journal of Molecular Sciences, 24(17). doi: 10.3390/ijms241713574

Skrzydlewska E, Łuczaj W, Biernacki M, Wójcik P, Jarocka Karpowicz I, Orehovec B, i sur.. Preliminary Comparison of Molecular Antioxidant and Inflammatory Mechanisms Determined in the Peripheral Blood Granulocytes of COVID-19 Patients. International Journal of Molecular Sciences [Internet]. 01.09.2023. [pristupljeno 19.12.2024.];24(17). doi: 10.3390/ijms241713574

E. Skrzydlewska, et al., "Preliminary Comparison of Molecular Antioxidant and Inflammatory Mechanisms Determined in the Peripheral Blood Granulocytes of COVID-19 Patients", International Journal of Molecular Sciences, vol. 24, br. 17, Rujan 2023. [Online]. Dostupno na: https://urn.nsk.hr/urn:nbn:hr:105:969039. [Citirano: 19.12.2024.]

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Naslov (engleski)Preliminary Comparison of Molecular Antioxidant and Inflammatory Mechanisms Determined in the Peripheral Blood Granulocytes of COVID-19 Patients
AutorElżbieta Skrzydlewska
AutorWojciech Łuczaj
AutorMichał Biernacki
AutorPiotr Wójcik
AutorIwona Jarocka-Karpowicz
AutorBiserka Orehovec
AutorBruno Baršić
AutorMarko Tarle
AutorMarta Kmet
AutorIvica Lukšić
AutorZlatko Marušić
AutorGeorg Bauer
AutorNeven Žarković
Autorova ustanovaSveučilište u Zagrebu
Medicinski fakultet
(KATEDRA ZA MAKSILOFACIJALNU KIRURGIJU SA STOMATOLOGIJOM)
Znanstveno / umjetničko
područje, polje i grana		BIOMEDICINA I ZDRAVSTVO
Kliničke medicinske znanosti
Kirurgija
Sažetak (engleski)
The aim of this study was to evaluate selected parameters of redox signaling and inflammation in the granulocytes of COVID-19 patients who recovered and those who died. Upon admission, the patients did not differ in terms of any relevant clinical parameter apart from the percentage of granulocytes, which was 6% higher on average in those patients who died. Granulocytes were isolated from the blood of 15 healthy people and survivors and 15 patients who died within a week, and who were selected post hoc for analysis according to their matching gender and age. They differed only in the lethal outcome, which could not be predicted upon arrival at the hospital. The proteins level (respective ELISA), antioxidant activity (spectrophotometry), and lipid mediators (UPUPLC–MS) were measured in the peripheral blood granulocytes obtained via gradient centrifugation. The levels of Nrf2, HO-1, NFκB, and IL-6 were higher in the granulocytes of COVID-19 patients who died within a week, while the activity of cytoplasmic Cu,Zn-SOD and mitochondrial Mn-SOD and IL-2/IL-10 were lower in comparison to the levels observed in survivors. Furthermore, in the granulocytes of those patients who died, an increase in pro-inflammatory eicosanoids (PGE2 and TXB2), together with elevated cannabinoid receptors 1 and 2 (associated with a decrease in the anti-inflammatory 15d-PGJ2), were found. Hence, this study suggests that by triggering transcription factors, granulocytes activate inflammatory and redox signaling, leading to the production of pro-inflammatory eicosanoids while reducing cellular antioxidant capacity through SOD, thus expressing an altered response to COVID-19, which may result in the onset of systemic oxidative stress, ARDS, and the death of the patient.
Ključne riječi (engleski)
Jezikengleski
Vrsta publikacijeZnanstveni rad - Izvorni znanstveni rad
Status objaveObjavljen
Vrsta recenzijeRecenziran
Verzija publikacijeObjavljena verzija rada (izdavačev PDF)
Naslov časopisaInternational Journal of Molecular Sciences
Brojčani podacivol. 24, br. 17, 13574
e-ISSN1422-0067
DOIhttps://doi.org/10.3390/ijms241713574
URN:NBNurn:nbn:hr:105:969039
Datum objave publikacije2023-09-01
URL dokumentahttps://www.mdpi.com/1422-0067/24/17/13574
Vrsta resursaTekst
Prava pristupaOtvoreni pristup
Uvjeti korištenjaIkona licence Imenovanje 4.0 međunarodna (CC BY 4.0)
Datum i vrijeme pohrane2024-05-22 11:38:31