prikaz prve stranice dokumenta Validation and application of caged Z-DEVD-aminoluciferin bioluminescence for assessment of apoptosis of wild type and TLR2-deficient mice after ischemic stroke
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Validation and application of caged Z-DEVD-aminoluciferin bioluminescence for assessment of apoptosis of wild type and TLR2-deficient mice after ischemic stroke
Journal of Photochemistry and Photobiology B: Biology, 253 (2024); 112871. https://doi.org/10.1016/j.jphotobiol.2024.112871
Josić Dominović, P.; Dobrivojević Radmilović, M.; Srakočić, S.; Mišerić, I.; Škokić, S.; Gajović, Srećko

Citirajte ovaj rad

Josić Dominović, P., Dobrivojević Radmilović, M., Srakočić, S., Mišerić, I., Škokić, S. i Gajović, S. (2024). Validation and application of caged Z-DEVD-aminoluciferin bioluminescence for assessment of apoptosis of wild type and TLR2-deficient mice after ischemic stroke. Journal of Photochemistry and Photobiology B: Biology, 253.. doi: 10.1016/j.jphotobiol.2024.112871

Josić Dominović, P., et al. "Validation and application of caged Z-DEVD-aminoluciferin bioluminescence for assessment of apoptosis of wild type and TLR2-deficient mice after ischemic stroke." Journal of Photochemistry and Photobiology B: Biology, vol. 253, 2024. https://doi.org/10.1016/j.jphotobiol.2024.112871

Josić Dominović, P., M. Dobrivojević Radmilović, S. Srakočić, I. Mišerić, S. Škokić i Srećko Gajović. "Validation and application of caged Z-DEVD-aminoluciferin bioluminescence for assessment of apoptosis of wild type and TLR2-deficient mice after ischemic stroke." Journal of Photochemistry and Photobiology B: Biology 253 (2024). https://doi.org/10.1016/j.jphotobiol.2024.112871

Josić Dominović, P., et al. (2024) 'Validation and application of caged Z-DEVD-aminoluciferin bioluminescence for assessment of apoptosis of wild type and TLR2-deficient mice after ischemic stroke', Journal of Photochemistry and Photobiology B: Biology, 253. doi: 10.1016/j.jphotobiol.2024.112871

Josić Dominović P, Dobrivojević Radmilović M, Srakočić S, Mišerić I, Škokić S, Gajović S. Validation and application of caged Z-DEVD-aminoluciferin bioluminescence for assessment of apoptosis of wild type and TLR2-deficient mice after ischemic stroke. Journal of Photochemistry and Photobiology B: Biology [Internet]. 04.2024. [pristupljeno 24.12.2024.];253. doi: 10.1016/j.jphotobiol.2024.112871

P. Josić Dominović, M. Dobrivojević Radmilović, S. Srakočić, I. Mišerić, S. Škokić i S. Gajović, "Validation and application of caged Z-DEVD-aminoluciferin bioluminescence for assessment of apoptosis of wild type and TLR2-deficient mice after ischemic stroke", Journal of Photochemistry and Photobiology B: Biology, vol. 253, Travanj 2024. [Online]. Dostupno na: https://urn.nsk.hr/urn:nbn:hr:105:271637. [Citirano: 24.12.2024.]

Za citiranje koristite ovu mrežnu adresu: https://urn.nsk.hr/urn:nbn:hr:105:271637
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Naslov (engleski)Validation and application of caged Z-DEVD-aminoluciferin bioluminescence for assessment of apoptosis of wild type and TLR2-deficient mice after ischemic stroke
AutorP. Josić Dominović
AutorM. Dobrivojević Radmilović
AutorS. Srakočić
AutorI. Mišerić
AutorS. Škokić
AutorSrećko Gajović
Autorova ustanovaSveučilište u Zagrebu
Medicinski fakultet
(HRVATSKI INSTITUT ZA ISTRAŽIVANJE MOZGA)
Autorova ustanovaSveučilište u Zagrebu
Medicinski fakultet
(KATEDRA ZA HISTOLOGIJU I EMBRIOLOGIJU)
Znanstveno / umjetničko
područje, polje i grana		BIOMEDICINA I ZDRAVSTVO
Temeljne medicinske znanosti
Citologija, histologija i embriologija
Sažetak (engleski)
Programmed cell death or apoptosis is a critically important mechanism of tissue remodeling and regulates conditions such as cancer, neurodegeneration or stroke. The aim of this research article was to assess the caged Z-DEVD-aminoluciferin substrate for in vivo monitoring of apoptosis after ischemic stroke in TLR2-deficient mice and their TLR2-expressing counterparts. Postischemic inflammation is a significant contributor to ischemic injury development and apoptosis, and it is modified by the TLR2 receptor. Caged Z-DEVD-aminoluciferin is made available for bioluminescence enzymatic reaction by cleavage with activated caspase-3, and therefore it is assumed to be capable of reporting and measuring apoptosis. Apoptosis was investigated for 28 days after stroke in mice which ubiquitously expressed the firefly luciferase transgene. Middle cerebral artery occlusion was performed to achieve ischemic injury, which was followed with magnetic resonance imaging. The scope of apoptosis was determined by bioluminescence with caged Z-DEVD-aminoluciferin, immunofluorescence with activated caspase-3, flow cytometry with annexin-V and TUNEL assay. The linearity of Z-DEVD-aminoluciferin substrate dose effect was shown in the murine brain. Z-DEVD-aminoluciferin was validated as a good tool for monitoring apoptosis following adequate adjustment. By utilizing bioluminescence of Z-DEVD-aminoluciferin after ischemic stroke it was shown that TLR2-deficient mice had lower post-stroke apoptosis than TLR2-expressing wild type mice. In conclusion, Z-DEVD-aminoluciferin could be a valuable tool for apoptosis measurement in living mice.
Ključne riječi (engleski)
Jezikengleski
Vrsta publikacijeZnanstveni rad - Izvorni znanstveni rad
Status objaveObjavljen
Vrsta recenzijeRecenziran
Verzija publikacijeObjavljena verzija rada (izdavačev PDF)
Naslov časopisaJournal of Photochemistry and Photobiology B: Biology
Brojčani podacivol. 253, 112871
p-ISSN1011-1344
DOIhttps://doi.org/10.1016/j.jphotobiol.2024.112871
URN:NBNurn:nbn:hr:105:271637
Datum objave publikacije2024-04
URL dokumentahttps://linkinghub.elsevier.com/retrieve/pii/S1011134424000319
Vrsta resursaTekst
Prava pristupaOtvoreni pristup
Uvjeti korištenjaIkona licence Imenovanje 4.0 međunarodna (CC BY 4.0)
Datum i vrijeme pohrane2024-11-11 09:21:23