prikaz prve stranice dokumenta Methodological Pitfalls of Investigating Lipid Rafts in the Brain: What Are We Still Missing?
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Methodological Pitfalls of Investigating Lipid Rafts in the Brain: What Are We Still Missing?
Biomolecules, 14 (2024), 2; 156. https://doi.org/10.3390/biom14020156
Mlinac-Jerković, Kristina; Kalanj-Bognar, Svjetlana; Heffer, Marija; Blažetić, Senka

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Mlinac Jerković, K., Kalanj Bognar, S., Heffer, M. i Blažetić, S. (2024). Methodological Pitfalls of Investigating Lipid Rafts in the Brain: What Are We Still Missing?. Biomolecules, 14. (2). doi: 10.3390/biom14020156

Mlinac Jerković, Kristina, et al. "Methodological Pitfalls of Investigating Lipid Rafts in the Brain: What Are We Still Missing?." Biomolecules, vol. 14, br. 2, 2024. https://doi.org/10.3390/biom14020156

Mlinac Jerković, Kristina, Svjetlana Kalanj Bognar, Marija Heffer i Senka Blažetić. "Methodological Pitfalls of Investigating Lipid Rafts in the Brain: What Are We Still Missing?." Biomolecules 14, br. 2 (2024). https://doi.org/10.3390/biom14020156

Mlinac Jerković, K., et al. (2024) 'Methodological Pitfalls of Investigating Lipid Rafts in the Brain: What Are We Still Missing?', Biomolecules, 14(2). doi: 10.3390/biom14020156

Mlinac Jerković K, Kalanj Bognar S, Heffer M, Blažetić S. Methodological Pitfalls of Investigating Lipid Rafts in the Brain: What Are We Still Missing?. Biomolecules [Internet]. 28.01.2024. [pristupljeno 15.11.2024.];14(2). doi: 10.3390/biom14020156

K. Mlinac Jerković, S. Kalanj Bognar, M. Heffer i S. Blažetić, "Methodological Pitfalls of Investigating Lipid Rafts in the Brain: What Are We Still Missing?", Biomolecules, vol. 14, br. 2, Siječanj 2024. [Online]. Dostupno na: https://urn.nsk.hr/urn:nbn:hr:105:213449. [Citirano: 15.11.2024.]

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Podaci o radu
Naslov (engleski)Methodological Pitfalls of Investigating Lipid Rafts in the Brain: What Are We Still Missing?
AutorKristina Mlinac-Jerković
AutorSvjetlana Kalanj-Bognar
AutorMarija Heffer
AutorSenka Blažetić
Autorova ustanovaSveučilište u Zagrebu
Medicinski fakultet
(KATEDRA ZA MEDICINSKU KEMIJU, BIOKEMIJU I KLINIČKU KEMIJU)
Znanstveno / umjetničko
područje, polje i grana		BIOMEDICINA I ZDRAVSTVO
Temeljne medicinske znanosti
Sažetak (engleski)
The purpose of this review is to succinctly examine the methodologies used in lipid raft research in the brain and to highlight the drawbacks of some investigative approaches. Lipid rafts are biochemically and biophysically different from the bulk membrane. A specific lipid environment within membrane domains provides a harbor for distinct raftophilic proteins, all of which in concert create a specialized platform orchestrating various cellular processes. Studying lipid rafts has proved to be arduous due to their elusive nature, mobility, and constant dynamic reorganization to meet the cellular needs. Studying neuronal lipid rafts is particularly cumbersome due to the immensely complex regional molecular architecture of the central nervous system. Biochemical fractionation, performed with or without detergents, is still the most widely used method to isolate lipid rafts. However, the differences in solubilization when various detergents are used has exposed a dire need to find more reliable methods to study particular rafts. Biochemical methods need to be complemented with other approaches such as live-cell microscopy, imaging mass spectrometry, and the development of specific non-invasive fluorescent probes to obtain a more complete image of raft dynamics and to study the spatio-temporal expression of rafts in live cells.
Ključne riječi (engleski)
Jezikengleski
Vrsta publikacijeZnanstveni rad - Pregledni rad
Status objaveObjavljen
Vrsta recenzijeRecenziran
Verzija publikacijeObjavljena verzija rada (izdavačev PDF)
Naslov časopisaBiomolecules
Brojčani podacivol. 14, br. 2, 156
e-ISSN2218-273X
DOIhttps://doi.org/10.3390/biom14020156
URN:NBNurn:nbn:hr:105:213449
Datum objave publikacije2024-01-28
ProjektŠifra: IP-2016-06-8636
Naziv (hrvatski): Molekularni biljezi vulnerabilnosti, adaptacije i plastičnosti neurona u akutnoj i kroničnoj ozljedi mozga
Naziv (engleski): Molecular markers of neuronal vulnerability, adaptation and plasticity in acute and chronic brain lesion
Kratica: NeuroReact
Voditelj: Svjetlana Kalanj-Bognar
Pravna nadležnost: Hrvatska
Financijer: Hrvatska zaklada za znanost
Linija financiranja: Research Projects
ProjektŠifra: IP-2014-09-2324
Naziv (hrvatski): Patofiziološke posljedice promjena sastava lipidnih splavi
Naziv (engleski): Pathophysiological consequences of lipid raft changes
Kratica: RafTuning
Voditelj: Marija Heffer
Pravna nadležnost: Hrvatska
Financijer: Hrvatska zaklada za znanost
Linija financiranja: Research Projects
URL dokumentahttps://www.mdpi.com/2218-273X/14/2/156
Vrsta resursaTekst
Prava pristupaOtvoreni pristup
Uvjeti korištenjaIkona licence Imenovanje 4.0 međunarodna (CC BY 4.0)
Datum i vrijeme pohrane2024-11-15 10:56:15