Development of the basic architecture of neocortical circuitry in the human fetus as revealed by the coupling spatiotemporal pattern of synaptogenesis along with microstructure and macroscale in vivo MR imaging

Znanstveni rad - Pregledni rad

• Autor: Ivica Kostović
• Autorova ustanova: Sveučilište u Zagrebu; Medicinski fakultet; (HRVATSKI INSTITUT ZA ISTRAŽIVANJE MOZGA)
• Znanstveno / umjetničko područje, polje i grana: BIOMEDICINA I ZDRAVSTVO; Temeljne medicinske znanosti; Neuroznanost

Sažetak (engleski)

In humans, a quantifiable number of cortical synapses appears early in fetal life. In this paper, we present a bridge across different scales of resolution and the distribution of synapses across the transient cytoarchitectonic compartments: marginal zone (MZ), cortical plate (CP), subplate (SP), and in vivo MR images. The tissue of somatosensory cortex (7–26 postconceptional weeks (PCW)) was prepared for electron microscopy, and classified synapses with a determined subpial depth were used for creating histograms matched to the histological sections immunoreacted for synaptic markers and aligned to in vivo MR images (1.5 T) of corresponding fetal ages (maternal indication). Two time periods and laminar patterns of synaptogenesis were identified: an early and midfetal two-compartmental distribution (MZ and SP) and a late fetal three-compartmental distribution (CP synaptogenesis). During both periods, a voluminous, synapse-rich SP was visualized on the in vivo MR. Another novel finding concerns the phase of secondary expansion of the SP (13 PCW), where a quantifiable number of synapses appears in the upper SP. This lamina shows a T2 intermediate signal intensity below the low signal CP. In conclusion, the early fetal appearance of synapses shows early differentiation of putative genetic mechanisms underlying the synthesis, transport and assembly of synaptic proteins. “Pioneering” synapses are likely to play a morphogenetic role in constructing of fundamental circuitry architecture due to interaction between neurons. They underlie spontaneous, evoked, and resting state activity prior to ex utero experience. Synapses can also mediate genetic and environmental triggers, adversely altering the development of cortical circuitry and leading to neurodevelopmental disorders.

• Ključne riječi (engleski): Female; Fetus; Humans; Magnetic Resonance Imaging; Neocortex / cytology; Neocortex / embryology; Neurogenesis / physiology; Somatosensory Cortex / cytology; Somatosensory Cortex / diagnostic imaging; Somatosensory Cortex / growth & development; Synapses / physiology; Synapses / ultrastructure
• Jezik: engleski
• Vrsta publikacije: Znanstveni rad - Pregledni rad
• Status objave: Objavljen
• Vrsta recenzije: Recenziran
• Verzija publikacije: Objavljena verzija rada (izdavačev PDF)
• Naslov časopisa: Brain Structure and Function
• Brojčani podaci: vol. 229, br. 9, str. 2339-2367
• e-ISSN: 1863-2661
• DOI: https://doi.org/10.1007/s00429-024-02838-9
• URN:NBN: urn:nbn:hr:105:913950
• Datum objave publikacije: 2024-08-05
• Projekt: Šifra: IP-2024-05-7157; Naziv (hrvatski): Ljudski prenatalni cerebralni neksus: neistražen prostorno-vremenski višerazmjerni kompartmentalni translacijski pristup; Naziv (engleski): Human prenatal cerebral nexus: uncharted spatiotemporal multiscale compartmental translational approach; Kratica: HUMANCOMPARTMENTOME; Voditelj: Ivica Kostović; Pravna nadležnost: Hrvatska; Financijer: Hrvatska zaklada za znanost; Linija financiranja: Research Projects
• URL dokumenta: https://link.springer.com/10.1007/s00429-024-02838-9
• Vrsta resursa: Tekst
• Prava pristupa: Otvoreni pristup
• Datum i vrijeme pohrane: 2025-04-01 08:24:28