prikaz prve stranice dokumenta Full pathogen characterisation: species identification including the detection of virulence factors and antibiotic resistance genes via multiplex DNA-assays
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Full pathogen characterisation: species identification including the detection of virulence factors and antibiotic resistance genes via multiplex DNA-assays
Scientific Reports, 11 (2021), 1; 6001. https://doi.org/10.1038/s41598-021-85438-5
Wolff, Noa; Hendling, Michaela; Schroeder, Fabian; Schönthaler, Silvia; Geiss, Andreas F.; Bedenić, Branka; Barišić, Ivan

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Wolff, N., Hendling, M., Schroeder, F., Schönthaler, S., Geiss, A. F., Bedenić, B. i Barišić, I. (2021). Full pathogen characterisation: species identification including the detection of virulence factors and antibiotic resistance genes via multiplex DNA-assays. Scientific Reports, 11. (1). doi: 10.1038/s41598-021-85438-5

Wolff, Noa, et al. "Full pathogen characterisation: species identification including the detection of virulence factors and antibiotic resistance genes via multiplex DNA-assays." Scientific Reports, vol. 11, br. 1, 2021. https://doi.org/10.1038/s41598-021-85438-5

Wolff, Noa, Michaela Hendling, Fabian Schroeder, Silvia Schönthaler, Andreas F. Geiss, Branka Bedenić i Ivan Barišić. "Full pathogen characterisation: species identification including the detection of virulence factors and antibiotic resistance genes via multiplex DNA-assays." Scientific Reports 11, br. 1 (2021). https://doi.org/10.1038/s41598-021-85438-5

Wolff, N., et al. (2021) 'Full pathogen characterisation: species identification including the detection of virulence factors and antibiotic resistance genes via multiplex DNA-assays', Scientific Reports, 11(1). doi: 10.1038/s41598-021-85438-5

Wolff N, Hendling M, Schroeder F, Schönthaler S, Geiss AF, Bedenić B, i sur.. Full pathogen characterisation: species identification including the detection of virulence factors and antibiotic resistance genes via multiplex DNA-assays. Scientific Reports [Internet]. 16.03.2021. [pristupljeno 19.04.2024.];11(1). doi: 10.1038/s41598-021-85438-5

N. Wolff, et al., "Full pathogen characterisation: species identification including the detection of virulence factors and antibiotic resistance genes via multiplex DNA-assays", Scientific Reports, vol. 11, br. 1, Ožujak 2021. [Online]. Dostupno na: https://urn.nsk.hr/urn:nbn:hr:105:422074. [Citirano: 19.04.2024.]

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Naslov (engleski)Full pathogen characterisation: species identification including the detection of virulence factors and antibiotic resistance genes via multiplex DNA-assays
AutorNoa Wolff
AutorMichaela Hendling
AutorFabian Schroeder
AutorSilvia Schönthaler
AutorAndreas F. Geiss
AutorBranka Bedenić
AutorIvan Barišić
Autorova ustanovaSveučilište u Zagrebu
Medicinski fakultet
(KATEDRA ZA MEDICINSKU MIKROBIOLOGIJU I PARAZITOLOGIJU)
Znanstveno / umjetničko
područje, polje i grana		BIOMEDICINA I ZDRAVSTVO
Kliničke medicinske znanosti
Medicinska mikrobiologija
Sažetak (engleski)
Antibiotic resistances progressively cause treatment failures, and their spreading dynamics reached an alarming level. Some strains have already been classified as highly critical, e.g. the ones summarised by the acronym ESKAPE (Enterococcus faecium, Staphylococcus aureus, Klebsiella pneumoniae, Acinetobacter baumannii, Pseudomonas aeruginosa and Enterobacter spp.). To restrain this trend and enable effective medication, as much information as possible must be obtained in the least possible time. Here, we present a DNA microarray-based assay that screens for the most important sepsis-relevant 44 pathogenic species, 360 virulence factors (mediate pathogenicity in otherwise non-pathogenic strains), and 409 antibiotic resistance genes in parallel. The assay was evaluated with 14 multidrug resistant strains, including all ESKAPE pathogens, mainly obtained from clinical isolates. We used a cost-efficient ligation-based detection platform designed to emulate the highly specific multiplex detection of padlock probes. Results could be obtained within one day, requiring approximately 4 h for amplification, application to the microarray, and detection.
Jezikengleski
Vrsta publikacijeZnanstveni rad - Izvorni znanstveni rad
Status objaveObjavljen
Vrsta recenzijeRecenziran
Verzija publikacijeObjavljena verzija rada (izdavačev PDF)
Naslov časopisaScientific Reports
Brojčani podacivol. 11, br. 1, 6001
e-ISSN2045-2322
DOIhttps://doi.org/10.1038/s41598-021-85438-5
URN:NBNurn:nbn:hr:105:422074
Datum objave publikacije2021-03-16
URL dokumentahttp://www.nature.com/articles/s41598-021-85438-5
Vrsta resursaTekst
Prava pristupaOtvoreni pristup
Uvjeti korištenjaIkona licence Imenovanje 4.0 međunarodna (CC BY 4.0)
Datum i vrijeme pohrane2021-06-18 08:05:59