Uroguanylin increases Ca2+ concentration in astrocytes via guanylate cyclase C-independent signaling pathway

Znanstveni rad - Izvorni znanstveni rad

• Autor: Nikola Habek
• Autor: Martina Ratko
• Autor: Aleksandra Dugandžić
• Autorova ustanova: Sveučilište u Zagrebu; Medicinski fakultet; (KATEDRA ZA FIZIOLOGIJU I IMUNOLOGIJU)
• Znanstveno / umjetničko područje, polje i grana: BIOMEDICINA I ZDRAVSTVO; Temeljne medicinske znanosti; Fiziologija čovjeka

Sažetak (engleski)

Aim: To investigate the cyclic guanosine monophosphate (cGMP)/guanylate cyclase C (GC-C)-independent signaling pathway in astrocytes, which are a suitable model due to their lack of GC-C expression.
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Methods: Patch clamp was performed and intracellular Ca2+ concentrations and pH were measured in primary astrocyte cultures and brain slices of wild type (WT) and GC-C knockout (KO) mice. The function of GC-C-independent signaling pathway in the cerebellum was determined by behavior tests in uroguanylin (UGN) KO and GC-C KO mice.
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Results: We showed for the first time that UGN changed intracellular Ca2+ levels in different brain regions of the mouse. In addition to the midbrain and hypothalamus, GC-C was expressed in the cerebral and cerebellar cortex. The presence of two signaling pathways in the cerebellum (UGN hyperpolarized Purkinje cells via GC-C and increased intracellular Ca2+ concentration in astrocytes) led to a different motoric function in GC-C KO and UGN KO mice, probably via different regulation of intracellular pH in astrocytes.
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Conclusion: The UGN effects on astrocytes via a Ca2+-dependent signaling pathway could be involved in the modulation of neuronal activity.

• Ključne riječi (engleski): Animals; Astrocytes* / metabolism; Calcium*; Guanylate Cyclase / metabolism; Mice; Natriuretic Peptides; Receptors, Peptide; Signal Transduction
• Jezik: engleski
• Vrsta publikacije: Znanstveni rad - Izvorni znanstveni rad
• Status objave: Objavljen
• Vrsta recenzije: Recenziran
• Verzija publikacije: Objavljena verzija rada (izdavačev PDF)
• Naslov časopisa: Croatian Medical Journal
• Brojčani podaci: vol. 62, br. 3, str. 250-263
• p-ISSN: 0353-9504
• e-ISSN: 1332-8166
• DOI: https://doi.org/10.3325/cmj.2021.62.250
• URN:NBN: urn:nbn:hr:105:258172
• Datum objave publikacije: 2021-06-30
• URL dokumenta: https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8275953/
• Vrsta resursa: Tekst
• Prava pristupa: Otvoreni pristup
• Datum i vrijeme pohrane: 2021-07-28 08:00:13