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Methylation Patterns of DKK1, DKK3 and GSK3β Are Accompanied with Different Expression Levels in Human Astrocytoma
Cancers, 13 (2021), 11; 2530. https://doi.org/10.3390/cancers13112530
Kafka, Anja; Bukovac, Anja; Brglez, Emilija; Jarmek, Ana-Marija; Poljak, Karolina; Brlek, Petar; Žarković, Kamelija; Njirić, Niko; Pećina-Šlaus, Nives

Citirajte ovaj rad

Kafka, A., Bukovac, A., Brglez, E., Jarmek, A. M., Poljak, K., Brlek, P. ... Pećina Šlaus, N. (2021). Methylation Patterns of DKK1, DKK3 and GSK3β Are Accompanied with Different Expression Levels in Human Astrocytoma. Cancers, 13. (11). doi: 10.3390/cancers13112530

Kafka, Anja, et al. "Methylation Patterns of DKK1, DKK3 and GSK3β Are Accompanied with Different Expression Levels in Human Astrocytoma." Cancers, vol. 13, br. 11, 2021. https://doi.org/10.3390/cancers13112530

Kafka, Anja, Anja Bukovac, Emilija Brglez, Ana Marija Jarmek, Karolina Poljak, Petar Brlek, Kamelija Žarković, Niko Njirić i Nives Pećina Šlaus. "Methylation Patterns of DKK1, DKK3 and GSK3β Are Accompanied with Different Expression Levels in Human Astrocytoma." Cancers 13, br. 11 (2021). https://doi.org/10.3390/cancers13112530

Kafka, A., et al. (2021) 'Methylation Patterns of DKK1, DKK3 and GSK3β Are Accompanied with Different Expression Levels in Human Astrocytoma', Cancers, 13(11). doi: 10.3390/cancers13112530

Kafka A, Bukovac A, Brglez E, Jarmek AM, Poljak K, Brlek P, i sur.. Methylation Patterns of DKK1, DKK3 and GSK3β Are Accompanied with Different Expression Levels in Human Astrocytoma. Cancers [Internet]. 21.05.2021. [pristupljeno 19.04.2024.];13(11). doi: 10.3390/cancers13112530

A. Kafka, et al., "Methylation Patterns of DKK1, DKK3 and GSK3β Are Accompanied with Different Expression Levels in Human Astrocytoma", Cancers, vol. 13, br. 11, Svibanj 2021. [Online]. Dostupno na: https://urn.nsk.hr/urn:nbn:hr:105:337868. [Citirano: 19.04.2024.]

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Naslov (engleski)Methylation Patterns of DKK1, DKK3 and GSK3β Are Accompanied with Different Expression Levels in Human Astrocytoma
AutorAnja Kafka
AutorAnja Bukovac
AutorEmilija Brglez
AutorAna-Marija Jarmek
AutorKarolina Poljak
AutorPetar Brlek
AutorKamelija Žarković
AutorNiko Njirić
AutorNives Pećina-Šlaus
Autorova ustanovaSveučilište u Zagrebu
Medicinski fakultet
(HRVATSKI INSTITUT ZA ISTRAŽIVANJE MOZGA)
Autorova ustanovaSveučilište u Zagrebu
Medicinski fakultet
(KATEDRA ZA PATOLOGIJU)
Znanstveno / umjetničko
područje, polje i grana		BIOMEDICINA I ZDRAVSTVO
Temeljne medicinske znanosti
Neuroznanost
Znanstveno / umjetničko
područje, polje i grana		BIOMEDICINA I ZDRAVSTVO
Kliničke medicinske znanosti
Patologija
Sažetak (engleski)
In the present study, we investigated genetic and epigenetic changes and protein expression levels of negative regulators of Wnt signaling, DKK1, DKK3, and APC as well as glycogen synthase kinase 3 (GSK3β) and β-catenin in 64 human astrocytomas of grades II–IV. Methylation-specific PCR revealed promoter methylation of DKK1, DKK3, and GSK3β in 38%, 43%, and 18% of samples, respectively. Grade IV comprised the lowest number of methylated GSK3β cases and highest of DKK3. Evaluation of the immunostaining using H-score was performed for β-catenin, both total and unphosphorylated (active) forms. Additionally, active (pY216) and inactive (pS9) forms of GSK3β protein were also analyzed. Spearman’s correlation confirmed the prevalence of β-catenin’s active form (rs = 0.634, p < 0.001) in astrocytoma tumor cells. The Wilcoxon test revealed that astrocytoma with higher levels of the active pGSK3β-Y216 form had lower expression levels of its inactive form (p < 0.0001, Z = −5.332). Changes in APC’s exon 11 were observed in 44.44% of samples by PCR/RFLP. Astrocytomas with changes of APC had higher H-score values of total β-catenin compared to the group without genetic changes (t = −2.264, p = 0.038). Furthermore, a positive correlation between samples with methylated DKK3 promoter and the expression of active pGSK3β-Y216 (rs = 0.356, p = 0.011) was established. Our results emphasize the importance of methylation for the regulation of Wnt signaling. Large deletions of the APC gene associated with increased β-catenin levels, together with oncogenic effects of both β-catenin and GSK3β, are clearly involved in astrocytoma evolution. Our findings contribute to a better understanding of the etiology of gliomas. Further studies should elucidate the clinical and therapeutic relevance of the observed molecular alterations.
Ključne riječi (engleski)
Jezikengleski
Vrsta publikacijeZnanstveni rad - Izvorni znanstveni rad
Status objaveObjavljen
Vrsta recenzijeRecenziran
Verzija publikacijeObjavljena verzija rada (izdavačev PDF)
Naslov časopisaCancers
Brojčani podacivol. 13, br. 11, 2530
e-ISSN2072-6694
DOIhttps://doi.org/10.3390/cancers13112530
URN:NBNurn:nbn:hr:105:337868
Datum objave publikacije2021-05-21
URL dokumentahttps://www.mdpi.com/2072-6694/13/11/2530
Vrsta resursaTekst
Prava pristupaOtvoreni pristup
Uvjeti korištenjaIkona licence Imenovanje 4.0 međunarodna (CC BY 4.0)
Datum i vrijeme pohrane2021-11-08 09:37:01